Journal: Plant Signaling & Behavior

Article Title: Dimerization of GTR1 regulates their plasma membrane localization

doi: 10.1080/15592324.2017.1334749

Figure Lengend Snippet: Glucosinolate or GA3 transport by GTR1-WT, -T135A and -T135D in Xenopus oocyte (A) Glucosinolate (4-methylthiobutyl glucosinolate: 4MTB) transport properties of GTR1-WT, -T135A and -T135D. Values are the means with SD (n = 3–7). (B) Gibberellin A3 (GA3) transport properties of GTR1-WT, -T135A and -T135D. Values are the means with SD (n = 3–7).

Article Snippet: For assay with 4MTB (Chromadex, USA), substrate concentration was 1 µM and incubation times were 0, 1, 2, 3 and 6 h. For assay with GA 3 (Wako, Japan), substrate concentration was 1 mM and incubation times were 0, 2, 4, 6 and 24 h. For determination of K m and V max , 2 oocytes were incubated in 50 µl of kulori-based solution (pH5.0) containing each substrate at 18°C.

Techniques:

Journal: Plant Signaling & Behavior

Article Title: Dimerization of GTR1 regulates their plasma membrane localization

doi: 10.1080/15592324.2017.1334749

Figure Lengend Snippet: Transport properties of GTR1-WT and mutants in Xenopus oocyte.

Article Snippet: For assay with 4MTB (Chromadex, USA), substrate concentration was 1 µM and incubation times were 0, 1, 2, 3 and 6 h. For assay with GA 3 (Wako, Japan), substrate concentration was 1 mM and incubation times were 0, 2, 4, 6 and 24 h. For determination of K m and V max , 2 oocytes were incubated in 50 µl of kulori-based solution (pH5.0) containing each substrate at 18°C.

Techniques: