Journal: bioRxiv

Article Title: Actinotrichia-independent developmental mechanisms of spiny rays facilitate the morphological diversification of Acanthomorpha fish fins

doi: 10.1101/2025.03.01.640274

Figure Lengend Snippet: a Distributions of Runx2-positive cells (green) and pSmad1/5/9-positive cells (magenta) in the spiny rays of a DF-st4 larvae stained by immunohistochemistry with DAPI (white). b pSmad1/5/9 signals (green) and bone morphologies (magenta) in a control fish and fish with inhibited BMP signaling. Spiny rays in the DF-st4 larvae were stained by immunohistochemistry with DAR4M (magenta) and DAPI (white). Blue arrows indicate cells embedded in the spiny-ray bone. All images are optical sections obtained with confocal microscopy.

Article Snippet: A whole-mount immunohistochemistry protocol was used to detect laminin (Sigma-Aldrich #L9393), TP63 (Abcam, #ab735), Runx2 (Santa Cruz Biotechnology, #sc-101145), and pSmad1/5/9 (Cell Signaling Technology, #13820).

Techniques: Staining, Immunohistochemistry, Control, Confocal Microscopy

Journal: bioRxiv

Article Title: Actinotrichia-independent developmental mechanisms of spiny rays facilitate the morphological diversification of Acanthomorpha fish fins

doi: 10.1101/2025.03.01.640274

Figure Lengend Snippet: a Lateral view of a larva, and a micro-CT scan of the skeleton. b Magnified CT image of the dorsal spine. c Distributions of Runx2-positive cells (green) and pSmad1/5/9-positive cells (magenta) in the dorsal spine, stained by immunohistochemistry with DAPI (white). Inset in upper left shows a magnified view of the dorsal spine region. Yellow arrow heads show examples of pSmad1/5/9 and Runx2-positive cells. Dorsal-spine bones are outlined in orange. The left-most picture is a confocal Z-stack image, and the others are optical sections obtained by confocal microscopy.

Article Snippet: A whole-mount immunohistochemistry protocol was used to detect laminin (Sigma-Aldrich #L9393), TP63 (Abcam, #ab735), Runx2 (Santa Cruz Biotechnology, #sc-101145), and pSmad1/5/9 (Cell Signaling Technology, #13820).

Techniques: Micro-CT, Staining, Immunohistochemistry, Confocal Microscopy

Journal: Acta biochimica et biophysica Sinica

Article Title: Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro.

doi: 10.1093/abbs/gmu040

Figure Lengend Snippet: Figure 4. Real-time PCR measurement of mRNA levels of HBMSCs cultured on TiO2/ZrO2 and TiO2 coatings in osteogenic medium and standard medium (A) Expression level of Runx2 in cells cultured in osteogenic medium was higher than that in cells cultured in standard medium at all time points. Cells cultured on TiO2/ZrO2 surfaces showed a higher Runx2 mRNA expression level than those on TiO2 coating in osteogenic medium at day 10 (*P , 0.05). (B) Expression level of Osterix in cells cultured in osteogenic medium was higher than that in cells cultured in standard medium at all time points. Cells cultured on TiO2/ZrO2 surfaces showed a higher Osterix mRNA expression level than those on TiO2 coating in osteogenic medium at days 7 and 10 (*P , 0.05). (C) Expression level of ALP in cells cultured in osteogenic medium was higher than that in cells cultured in standard medium at most time points (*P , 0.05). (D) Expression level of OPN in cells cultured in osteogenic medium was higher than that in cells cultured in standard medium at all time course (*P , 0.05).

Article Snippet: After co-incubation with primary rabbit polyclonal anti-human osteopontin antibodies (1 : 100; Proteintech Group, Chicago, USA) and primary mouse monoclonal anti-human Runx2 antibodies (1 : 100; ProSci, Poway, USA) overnight at 48C, all the samples were then washed with PBS and co-incubated with Cy3-labeled goat anti-rabbit IgG (1:1000; Beyotime, Beijing, China) and fluorescein (FITC)-labeled goat anti-mouse IgG (1:1000; Beyotime) for 1 h at RT.

Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Expressing

Journal: Acta biochimica et biophysica Sinica

Article Title: Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro.

doi: 10.1093/abbs/gmu040

Figure Lengend Snippet: Figure 5. Immunofluorescence for in vitro osteogenic differentiation of HBMSCs Immunofluorescence labeling of Runx2 (FITC, Green), OPN (Cy3, Red) and nucleus (DAPI, Blue) of HBMSCs on TiO2/ZrO2 (A–E) and TiO2 coatings (F–J) cultured in osteogenic/standard medium for 7 days. HBMSCs cultured on TiO2/ZrO2 coating (A: DAPI image, B: Cy3 image, C: FITC image, D: merged image) and TiO2 coating (F: DAPI image, G: Cy3 image, H: FITC image, I: merged image) in osteogenic medium exhibited a more intense fluorescence of Cy3 and FITC compared with cells cultured in standard medium (E, J: merged images).

Article Snippet: After co-incubation with primary rabbit polyclonal anti-human osteopontin antibodies (1 : 100; Proteintech Group, Chicago, USA) and primary mouse monoclonal anti-human Runx2 antibodies (1 : 100; ProSci, Poway, USA) overnight at 48C, all the samples were then washed with PBS and co-incubated with Cy3-labeled goat anti-rabbit IgG (1:1000; Beyotime, Beijing, China) and fluorescein (FITC)-labeled goat anti-mouse IgG (1:1000; Beyotime) for 1 h at RT.

Techniques: Immunofluorescence, In Vitro, Labeling, Cell Culture, Fluorescence