rtg Search Results


rtg p1 cells  (ATCC)
93
ATCC rtg p1 cells
Rtg P1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/RTG-P1/pmc07922653-112-0-7
Average 93 stars, based on 1 article reviews
rtg p1 cells - by Bioz Stars, 2026-09
93/100 stars
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illustra tm hot start mix rtg pcr beads  (Cytiva Europe)
93
Cytiva Europe illustra tm hot start mix rtg pcr beads
Illustra Tm Hot Start Mix Rtg Pcr Beads, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/illustra+Hot+Start+Mix+RTG/pmc09311398-257-5-13
Average 93 stars, based on 1 article reviews
illustra tm hot start mix rtg pcr beads - by Bioz Stars, 2026-09
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rtg2 fibroblast cell line  (ATCC)
94
ATCC rtg2 fibroblast cell line
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg2 Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/RTG-2/pm28360916-95-15-19
Average 94 stars, based on 1 article reviews
rtg2 fibroblast cell line - by Bioz Stars, 2026-09
94/100 stars
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rtg 2  (ATCC)
94
ATCC rtg 2
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg 2, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/RTG-2%3B+Mixed+Ovaries%2FTestes%3B+Trout/pmc04313277-44-63-64
Average 94 stars, based on 1 article reviews
rtg 2 - by Bioz Stars, 2026-09
94/100 stars
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pcmv6 mouse unc119 plasmid  (OriGene)
90
OriGene pcmv6 mouse unc119 plasmid
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Pcmv6 Mouse Unc119 Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/Unc119+(NM_011676)+Mouse+Tagged+ORF+Clone+Lentiviral+Particle/pmc03508144-160-9-12
Average 90 stars, based on 1 article reviews
pcmv6 mouse unc119 plasmid - by Bioz Stars, 2026-09
90/100 stars
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rtg investigator  (Real Time Genomics)
90
Real Time Genomics rtg investigator
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg Investigator, supplied by Real Time Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rtg+investigator/us08209130-512-30-33
Average 90 stars, based on 1 article reviews
rtg investigator - by Bioz Stars, 2026-09
90/100 stars
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rtg population caller  (Real Time Genomics)
90
Real Time Genomics rtg population caller
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg Population Caller, supplied by Real Time Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rtg+population+caller/pmc04482534-133-4-1
Average 90 stars, based on 1 article reviews
rtg population caller - by Bioz Stars, 2026-09
90/100 stars
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rtg  (SEMATECH Inc)
90
SEMATECH Inc rtg
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg, supplied by SEMATECH Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rtg+correlator/pm10428794-71-24-28
Average 90 stars, based on 1 article reviews
rtg - by Bioz Stars, 2026-09
90/100 stars
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rtg  (Analiza Inc)
90
Analiza Inc rtg
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg, supplied by Analiza Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rtg/10__2478_slash_v10172___011___0125___6-23-19-0
Average 90 stars, based on 1 article reviews
rtg - by Bioz Stars, 2026-09
90/100 stars
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rubber tired gantry (rtg) single-plane digital subtraction angiography (dsa) suite  (Siemens AG)
90
Siemens AG rubber tired gantry (rtg) single-plane digital subtraction angiography (dsa) suite
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rubber Tired Gantry (Rtg) Single Plane Digital Subtraction Angiography (Dsa) Suite, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rubber+tired+gantry++rtg++single+plane+digital+subtraction+angiography++dsa++suite/pmc05429154-49-14-13
Average 90 stars, based on 1 article reviews
rubber tired gantry (rtg) single-plane digital subtraction angiography (dsa) suite - by Bioz Stars, 2026-09
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rtg cgmap  (Real Time Genomics)
90
Real Time Genomics rtg cgmap
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg Cgmap, supplied by Real Time Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rtg+map+v3+7/pm22975759-43-210-204
Average 90 stars, based on 1 article reviews
rtg cgmap - by Bioz Stars, 2026-09
90/100 stars
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rtg lamp ypo evo 2250  (YXLON International GmbH)
90
YXLON International GmbH rtg lamp ypo evo 2250
Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and <t>RTG2</t> <t>(fibroblasts)</t> cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).
Rtg Lamp Ypo Evo 2250, supplied by YXLON International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rtg/rtg+lamp+ypo+evo+2250/pmc11051480-119-39-41
Average 90 stars, based on 1 article reviews
rtg lamp ypo evo 2250 - by Bioz Stars, 2026-09
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Image Search Results


Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and RTG2 (fibroblasts) cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).

Journal: Frontiers in immunology

Article Title: B Cell-Activating Factor Regulates Different Aspects of B Cell Functionality and Is Produced by a Subset of Splenic B Cells in Teleost Fish.

doi: 10.3389/fimmu.2017.00295

Figure Lengend Snippet: Figure 2 | B cell-activating factor (BAFF) expression in rainbow trout in physiological conditions. BAFF transcription levels were analyzed through real-time PCR in rainbow trout tissues (A) and FACS isolated cell subsets and in the RTS11 (macrophages) and RTG2 (fibroblasts) cell lines (B) the relative expression to the endogenous control EF-1α was calculated for each sample (shown as mean + SD, n = 6) (HK, head kidney; DCs, dendritic cells; Per, peritoneum). (C) Rainbow trout leukocytes isolated from spleen were stained with an anti-IgM mAb together with an anti-BAFF pAb and analyzed by flow cytometry. Lymphoid (blue gate) and myeloid (red gate) populations were gated and, then, IgM− (orange gate) and IgM+ (green gate) cells were further selected from the lymphoid population. A representative histogram showing the level of BAFF expression in each population is shown. The average percentage of BAFF+ cells within each compartment was also calculated (D) as well as the mean fluorescence intensity of BAFF on those populations (E) (shown as mean + SD, n = 9, three independent experiments containing three animals each). (F) The percentages of myeloid, lymphoid IgM−, and lymphoid IgM+ cells within the BAFF+ compartment were also calculated and are plotted as pie charts (mean ± SD, n = 9, three independent experiments containing three animals each). Dead cells were not excluded during the flow cytometry analysis, but cell viability was higher than 99% for IgM+ B cells and higher than 95% for other lymphocytes on these experimental settings (Figure S4B in Supplementary Material). (G) Total leukocytes from spleen were plated onto poly-l-lysine-coated glass slides, fixed and labeled with anti-IgM (red) and anti-BAFF (green) antibodies, then counterstained with DAPI (blue), and analyzed by fluorescence microscopy. Images from one representative experiment are shown (scale bar, 1 μm).

Article Snippet: RNA was also extracted from the RTS11 rainbow trout monocyte–macrophage cell line (46) and the RTG2 fibroblast cell line (ATCC CCL-55) following the same procedure.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Isolation, Control, Staining, Flow Cytometry, Fluorescence, Labeling, Microscopy