Journal: Translational Neurodegeneration

Article Title: ImmunoPET imaging of amyloid-beta in a rat model of Alzheimer’s disease with a bispecific, brain-penetrating fusion protein

doi: 10.1186/s40035-022-00324-y

Figure Lengend Snippet: Generation of bispecific fusion proteins with OX26 affinity variants. a Biacore analysis of affinity to rTfR for OX26 5 , OX26 76 and commercial OX26 LSBio . b Schematic of the IEDDA reaction to generate OX26-F(ab′) 2 -Bapi bispecific fusion proteins. c, d SDS-PAGE analysis of OX26 5 -F(ab′) 2 -Bapi ( c ) and OX26 76 -F(ab′) 2 -Bapi ( d ). Lanes 1, molecular weight ladder; 2, parental OX26 IgG; 3, F(ab′) 2 -Bapi; 4–5, preparative SEC fractions for the final pool of bispecific fusion protein. e, f ELISA analysis of OX26 5 -F(ab′) 2 -Bapi and OX26 76 -F(ab′) 2 -Bapi before and after 125 I-labelling binding to rTfR ( e ) and Aβ ( f ). RU, response unit; OD, optical density

Article Snippet: Indirect ELISA was performed to assess the potential effects of conjugation and radiolabelling on the binding of OX26 5 -F(ab′) 2 -Bapi or OX26 76 -F(ab′) 2 -Bapi to rTfR and Aβ, using plates coated with 13.3 nM of rTfR or 50 nM of Aβ (Innovagen, Lund, Sweden), respectively.

Techniques: SDS Page, Molecular Weight, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Translational Neurodegeneration

Article Title: ImmunoPET imaging of amyloid-beta in a rat model of Alzheimer’s disease with a bispecific, brain-penetrating fusion protein

doi: 10.1186/s40035-022-00324-y

Figure Lengend Snippet: EC50 of antibody binding to rTfR or Aβ in direct ELISA

Article Snippet: Indirect ELISA was performed to assess the potential effects of conjugation and radiolabelling on the binding of OX26 5 -F(ab′) 2 -Bapi or OX26 76 -F(ab′) 2 -Bapi to rTfR and Aβ, using plates coated with 13.3 nM of rTfR or 50 nM of Aβ (Innovagen, Lund, Sweden), respectively.

Techniques: Binding Assay