Journal: bioRxiv

Article Title: Time elapsed between Zika and dengue virus infections affects antibody and T cell responses

doi: 10.1101/621094

Figure Lengend Snippet: ( a ) DENV-2 RNA kinetics in ZIKV-immune and naïve animals at baseline, day 1 to day 10, and day 15 after DENV infection. RNA genome copies (Log10) per ml of serum were measured by qRT-PCR. Symbols represent individual animals per cohort: blue squares (ZIKVPF-10mo), orange squares (ZIKVPR-2mo) and black circles (Naïve). Box and whiskers show the distribution of log-transformed values per group per timepoint. Boxes include the mean value per group while whiskers depict the minimum and maximum values for each group. Cutted line mark the limit of detection (20 genomes copies). Statistically significant differences between groups were determined using Two-Way Anova adjusted for Tukey’s multiple comparisons test including 12 families, and 3 comparisons per family. ( b ) Total days that DENV-2 RNAemia was detected for each animal within cohorts. Bars represent mean days per cohort. Source data are provided as a Source Data file.

Article Snippet: RNAemia levels were measured by a One-Step qRT-PCR detection kit (Oasig, Primerdesign Ltd., UK) and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol (catalog No. oasig-onestep).

Techniques: Infection, Quantitative RT-PCR, Transformation Assay