Journal: Journal of Neuroinflammation

Article Title: Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat

doi: 10.1186/s12974-017-0822-9

Figure Lengend Snippet: List of primary and secondary antibodies used for Western blot analysis

Article Snippet: TNF-α , rabbit , Sino Biological , 80045-RP02 , 1:500 , Overnight 4 °C.

Techniques: Western Blot, Incubation

Journal: Journal of Neuroinflammation

Article Title: Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat

doi: 10.1186/s12974-017-0822-9

Figure Lengend Snippet: Suppressed activation of glial cells and TNF-α production by inhibition of MyD88 in rat SDH after CCI. a Immunostaining showing inhibitory effects of MIP on activation of microglial cells (IBA1), and astrocytes (GFAP). Scale bar: 20 μm. b Western blot showing inhibitory effects of MIP on CCI-induced increased protein level of IBA1, GFAP, and TNF-α. c Data summary of B. Others are the same as Fig.

Article Snippet: TNF-α , rabbit , Sino Biological , 80045-RP02 , 1:500 , Overnight 4 °C.

Techniques: Activation Assay, Inhibition, Immunostaining, Western Blot

Journal: Journal of Neuroinflammation

Article Title: Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat

doi: 10.1186/s12974-017-0822-9

Figure Lengend Snippet: Schematic illustration demonstrates MyD88-dependent signaling pathways of neuropathic pain induced by CCI. Nerve injury produces abundant HMGB1and IL-1β in the DRG and SDH. The binding of HMGB1 and IL-1β to their receptors (TLR2/4 andIL-1R, respectively) activates MyD88 in the DRG and SDH, which phosphorylate NF-κB p65 and ERK. Phosphorylated NF-κB p65 subsequently enter the nucleus to regulate the expression of proinflammation cytokines such as TNF-α. Phosphorylated ERK enter the nucleus to induce transcription factors such as AP-1, which regulates the expression of certain cytokines and activates glial cells. All these signaling events consequently result in central and peripheral sensitizations that produce neuropathic pain

Article Snippet: TNF-α , rabbit , Sino Biological , 80045-RP02 , 1:500 , Overnight 4 °C.

Techniques: Binding Assay, Expressing

Journal: Vaccine

Article Title: The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection

doi: 10.1016/j.vaccine.2016.11.064

Figure Lengend Snippet: Description of the N-terminal domain (NTD) immunogen and vaccination schedule. (A) The location of the NTD protein on the Middle East respiratory syndrome coronavirus MERS-CoV spike (S) protein. The recombinant (r)NTD protein consists of 336 amino acid (aa) residues (18–353) of S protein. A gp67 signal peptide (SP) was added to the N terminus for expression of the rNTD protein. (B) Purified rNTD protein detected by SDS-PAGE (left) and Western blot (right). The purified rNTD protein was separated by a 10% SDS-PAGE and stained with 0.25% Coomassie brilliant blue. Anti-NTD polyclonal antibody and infrared ray-labeled secondary antibody were used for the Western blot assay. Lane 1: protein molecular weight marker; lane 2: purified rNTD protein. (C). Vaccination schedule and detection. Mice received three vaccinations consisting of 5 or 10 μg of rNTD protein combined with adjuvants at 4-week intervals. Sera were collected at the indicated times to analyze the humoral immune response. Six mice from each group were sacrificed 2 weeks after the last immunization. The spleens were harvested for enzyme-linked immunospot (ELISpot), intracellular cytokine staining (ICS), and cytometric bead array (CBA) assays. In parallel experiments, the remaining mice were challenged with MERS-CoV to detect the protective effect elicited by the rNTD protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Rabbit-serum-derived polyclonal antibody against nucleoprotein (cat: 100213-RP02; Sino Biological Inc., Beijing, CHN) was incubated with the sections at 1:1000 dilution; goat anti-rabbit (cat: pv-9001; ZSGB-Bio, Beijing, CHN) secondary antibody was used at 1:2000, and sections were evaluated using light microscopy.

Techniques: Recombinant, Expressing, Purification, SDS Page, Western Blot, Staining, Labeling, Molecular Weight, Marker, Enzyme-linked Immunospot

Journal: Vaccine

Article Title: The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection

doi: 10.1016/j.vaccine.2016.11.064

Figure Lengend Snippet: IHC detection of virus antigen expression in mouse tissue after challenge with MERS-CoV. Lung (A–C) and trachea (D–F) sections were assessed using rabbit polyclonal antibody to MERS-CoV nucleoprotein (NP) 3 days after the MERS-CoV challenge. The dark purple spot marked the inflammatory cell infiltration, and the brown particle marked the antigen of MERS-CoV. The MERS-CoV was located mainly in the trachea. Additionally, the lung tissue showed MERS-CoV expression in all immunized groups.

Article Snippet: Rabbit-serum-derived polyclonal antibody against nucleoprotein (cat: 100213-RP02; Sino Biological Inc., Beijing, CHN) was incubated with the sections at 1:1000 dilution; goat anti-rabbit (cat: pv-9001; ZSGB-Bio, Beijing, CHN) secondary antibody was used at 1:2000, and sections were evaluated using light microscopy.

Techniques: Expressing

Journal: Advanced materials (Deerfield Beach, Fla.)

Article Title: Tunable Fano-resonant Metasurfaces on a Disposable Plastic-template for Multi-modal and Multiplex Biosensing

doi: 10.1002/adma.201907160

Figure Lengend Snippet: Surface sensitivity of plastic-templated metasurface for layer-by-layer modifications. a Summary of surface chemistry steps. b Resonance peak wavelength shifts demonstrated on surfaces etched for 30, 60 and 90 sec. c Layer-by-layer binding response of bio-molecules on the 60 sec etched surface is monitored in real-time. d-f Resonance wavelength shifts as a function various concentrations of protein G, anti-gp120 antibody and rec-gp120 protein are represented via Box-Whisker plots. I-shaped box indicates 25th and 75th, red-line median, and whiskers the 95th and 5th percentiles. Dots represent data points for each concentration. Non-parametric Kruskal-Wallis analysis followed by Dunn’s multiple comparison test was performed to evaluate the collected data for each concentration at Protein G, anti-gp120 antibody and rec. gp120 protein experiments. The minimum, first quartile, median, third quartile, and maximum of each concentration were presented in Supplementary Table 1. In Protein G experiment, statistical difference was observed between 75 and 1000 μg/mL concentrations (n=3, p<0.05). In anti-gp120 antibody experiments, statistical difference was observed between 30 and 75 μg/mL concentrations (n=3, p<0.05). In rec-gp120 protein experiments, statistical difference was observed between 25 and 200 μg/mL concentrations (n=3, p<0.05).

Article Snippet: This step was followed by a 100 μL PBS wash. (2) Anti-gp120 antibody layer: 100 μL of anti-gp120 (immunoaffinity purified rabbit polyclonal IgG, 1 mg/mL, #11233-RP02, Sino Biological, Pennsylvania, US) was captured on the protein G layer and the surface was washed using 100 μL PBS. (3) HIV capture: The functionalized metasurface was used to capture recombinant gp120 proteins and HIV-1 particles.

Techniques: Binding Assay, Whisker Assay, Concentration Assay

Journal: iScience

Article Title: Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degeneration

doi: 10.1016/j.isci.2023.107939

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-PEDF , Sino Biological , Cat#11104-RP02.

Techniques: Virus, Recombinant, Transfection, Protease Inhibitor, Plasmid Preparation, Gel Extraction, Software