Journal: Molecular oncology

Article Title: Dying tumor cells stimulate proliferation of living tumor cells via caspase-dependent protein kinase Cδ activation in pancreatic ductal adenocarcinoma

doi: 10.1016/j.molonc.2014.07.024

Figure Lengend Snippet: Activated PKCδ stimulating living tumor cell growth. (a) Western blot showed that PKCδ but not ε or θ was cleaved in irradiated Panc1 cells peaked at day 3 and continued to day 6. (b) The pan PKC inhibitor GF109203x significantly ...

Article Snippet: PKC pan inhibitor GF109203x and PKCδ specific inhibitor rottlerin were purchased from Tocris Bioscience (Bristol, UK).

Techniques: Western Blot, Irradiation

Journal: FEBS letters

Article Title: Activation of CEA-CAM-1-mediated cell adhesion via CD98: involvement of PKCdelta.

doi: 10.1016/s0014-5793(03)00924-4

Figure Lengend Snippet: Fig. 5. Inhibition of the cell aggregation and translocation of PKCN by rottlerin. A: B/C-CAM-1 and B/LFA-1 cells were cultured with mAb 14.37 in the absence or presence of rottlerin (5 WM) or wort- mannin (50 nM) for 2 h. F9 cells were cultured in the presence of mAb 14.37 for 24 h with or without rottlerin (5 WM), and treated with trypsin/EDTA. B: F9 cells were cultured in the absence or presence of mAb 14.37 for 5 h with or without rottlerin, and immu- nostained with anti-PKCN antibody (original magni¢cation, U600). Note the di¡erence in cell size among the three groups.

Article Snippet: Anti-PKCN antibody (Santa Cruz), rottlerin (Sigma) and wortmannin (Sigma) were purchased commercially.

Techniques: Inhibition, Translocation Assay, Cell Culture