Journal: Frontiers in Pharmacology

Article Title: Norcantharidin ameliorates estrogen deficient-mediated bone loss by attenuating the activation of extracellular signal-regulated kinase/ROS/NLRP3 inflammasome signaling

doi: 10.3389/fphar.2022.1019478

Figure Lengend Snippet: NCTD reduced the content of ROS and inhibited the formation of inflammasome in BMM cells. (A) During BMM differentiating into osteoclast with RANKL, ROS kit was used to detect the ROS content in BMM. (B) The ROS observed under fluorescence microscope was quantified. (C) Western blotting was used to measure NLRP3 and ASC expression level in BMM induced osteoclasts at day 0, 1, 3, and 5. (D) The NLRP3 and ASC bands of Western blotting were quantified. All experiments were repeated independently for three times. Values are expressed as mean ± SD; * p < 0.05, ** p < 0.01 vs. the control group.

Article Snippet: ROS Kit (Boster Bio, Wuhan, China) was used to detect reactive oxygen species.

Techniques: Fluorescence, Microscopy, Western Blot, Expressing

Journal: Biochemistry and Biophysics Reports

Article Title: Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI–H292 human lung epithelial cells

doi: 10.1016/j.bbrep.2021.101124

Figure Lengend Snippet: Effects of integrin β1 depletion on cellular and secreted mucin 5AC (MUC5AC) and ROS levels in NCI–H292 cells. A, NCI–H292 cells were transfected with integrin β1 siRNA (β1 siRNA, Santa Cruz Biotechnology) or control siRNA (CNTL), and cultured on a 96-well plate. Integrin β1 and β-actin protein levels were detected via immunoblotting and measured using ChemiDoc. B, MUC5AC levels in the integrin β1 siRNA (β1 siRNA, Santa Cruz Biotechnology) or control siRNA (CNTL), transfected cells were detected using the dot blot method and measured (cellular). C, MUC5AC levels in the culture medium of the in the integrin β1 siRNA (β1 siRNA, Santa Cruz Biotechnology) or control siRNA (CNTL), transfected cells were detected via the dot blot method and measured (secreted). D, MUC5AC levels in the integrin β1 siRNA (β1 siRNA, Thermo Fisher) or control siRNA (CNTL), transfected cells were detected using the dot blot method and measured (cellular). E, MUC5AC levels in the culture medium of the integrin β1 siRNA (β1 siRNA, Thermo Fisher) or control siRNA (CNTL), transfected cells were detected via the dot blot method and measured (secreted). F, ROS levels in the integrin β1 siRNA (β1 siRNA, Santa Cruz Biotechnology) or control siRNA (CNTL), transfected cells were detected by hydrogen peroxide/peroxidase assay (cellular). Fold changes were based on control cells (mean ± standard deviation, n = 5). Fold changes were normalized for cell numbers. Student's t-test was used to obtain p -values. Asterisks indicate statistical significance, * p < 0.05. Representative results for three independent experiments are presented.

Article Snippet: F, ROS levels in the integrin β1 siRNA (β1 siRNA, Santa Cruz Biotechnology) or control siRNA (CNTL), transfected cells were detected by hydrogen peroxide/peroxidase assay (cellular).

Techniques: Transfection, Control, Cell Culture, Western Blot, Dot Blot, Standard Deviation

Journal: Biochemistry and Biophysics Reports

Article Title: Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI–H292 human lung epithelial cells

doi: 10.1016/j.bbrep.2021.101124

Figure Lengend Snippet: A hypothetical model of integrin β1 reduces MUC5AC expression via ERK activation and ROS formation. The arrow-headed lines and bar-headed lines indicate activation and inhibition, respectively.

Article Snippet: F, ROS levels in the integrin β1 siRNA (β1 siRNA, Santa Cruz Biotechnology) or control siRNA (CNTL), transfected cells were detected by hydrogen peroxide/peroxidase assay (cellular).

Techniques: Expressing, Activation Assay, Inhibition