Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 1. Experimental design used for the induction of acute and chronic pancreatitis models in rats. The acute model is induced with a tail vein injection of DBTC and nociceptive hypersensitivity peaks at 1 week. Hypersensitivity develops within 3 weeks in Fisher rats fed a high fat and alcohol diet and persists for 7 weeks. In both models, referred pain is present on the rat’s abdomen and in a cutaneous band extending dorsally to the shoulder and neck (hatched region).

Article Snippet: High fat commercial diet with 28% fat (LD101A with LD104, TestDiet, Richmond, IN).

Techniques: Injection

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 2. Photomicrographs of immunohistochemical staining of regulated on activation, normal T cells expressed and secreted (RANTES) in the pancreas ( a ) from a naïve rat, ( b ) from a rat 1 week after dibutyltin dichloride (DBTC)-induced pancreatitis and ( c ) from an animal 10 weeks after induction of pancreatitis with a diet of high fat and alcohol (6%). RANTES is a chemokine attracting infl ammatory cells to the infl amed pancreas. The small particles evident in the widened ducts ( spaces ) surrounding the pancreatic exocrine cells are the invading infl ammatory cells (leukocytes) Scale Bar: 50 um.

Article Snippet: High fat commercial diet with 28% fat (LD101A with LD104, TestDiet, Richmond, IN).

Techniques: Immunohistochemical staining, Staining, Activation Assay

Journal: Methods in Molecular Biology

Article Title: Pain Research

doi: 10.1007/978-1-61779-561-9

Figure Lengend Snippet: Fig. 5. The response times to hotplate are shown for control rats and rats fed the high fat and alcohol diet for 4 weeks. Responses times that are signifi cantly less for the animals with pancreatitis for 3–6 weeks indicate hypersensitivity of the animals fed the high fat and alcohol diet.

Article Snippet: High fat commercial diet with 28% fat (LD101A with LD104, TestDiet, Richmond, IN).

Techniques: Control

Journal: Frontiers in physiology

Article Title: Gut microbiota depletion minimally affects the daily voluntary wheel running activity and food anticipatory activity in female and male C57BL/6J mice.

doi: 10.3389/fphys.2023.1299474

Figure Lengend Snippet: FIGURE 1 The experimental procedure for microbiota depletion and feeding schedule. The days of beginning of ad libitum feeding (ALI, ALII), timed restricted feeding (TRF), food deprivation (FD) and collection of cecum and feces are indicated with the arrows.

Article Snippet: Extracted fecal DNA samples were shipped to Transnetyx Microbiome (Cordova, TN) for library preparation and whole-genome sequencing.

Techniques:

Journal: Frontiers in physiology

Article Title: Gut microbiota depletion minimally affects the daily voluntary wheel running activity and food anticipatory activity in female and male C57BL/6J mice.

doi: 10.3389/fphys.2023.1299474

Figure Lengend Snippet: FIGURE 5 Relative abundances of microbiota taxa from control and antibiotic-treated mice. Plot representing bacterial load in control (A) and antibiotics- treated (B) mice. Antibiotics depleted majority of gut microbiota. The “Mouse proportion” represents the number of reads that map to the One Codex database mouse genome divided by the total number of reads, while the “Bacterial proportion” represents the number of reads that map to bacteria at the taxonomic rank divided by the total number of reads. The “Other” category includes any reads classified as non-bacterial microorganisms. Taxonomic analysis showing gut microbiota population shifts at Family level. Relative abundance of top 15 families of microbial taxa in control (C), and antibiotics-treated (D) are shown. Lachnospiraceae (indicated as green) was present in all control mice but depleted in antibiotics treated mice. Erysipelotrichaceae was not detected in both control and antibiotics-treated mice. Top 15 orders and genus were shown in Supplementary Figure S5A–D. The number in the brackets represents NCBI taxonomic ID.

Article Snippet: Extracted fecal DNA samples were shipped to Transnetyx Microbiome (Cordova, TN) for library preparation and whole-genome sequencing.

Techniques: Control, Bacteria