Journal: bioRxiv

Article Title: Erbb4 deletion from fast-spiking interneurons causes psychosis-relevant neuroimaging phenotypes

doi: 10.1101/2022.03.07.483347

Figure Lengend Snippet: Representative hippocampal regions of interests. (A) Regions of interests sampled for [ 3 H]Ro15-4513 and [ 3 H]flumazenil. (B) Regions of interests sampled for [ 3 H]UCB-J. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: middle hippocampus dentate gyrus.

Article Snippet: To quantify density of α5GABA A R – sections were incubated for 60 minutes at room temperature in 2nM [ 3 H]Ro15-4513 (Perkin Elmer, NET925250UC), or in 2nM [ 3 H]Ro15-4513 with 10 µM bretazenil (Sigma, B6434) for nonspecific binding.

Techniques:

Journal: bioRxiv

Article Title: Erbb4 deletion from fast-spiking interneurons causes psychosis-relevant neuroimaging phenotypes

doi: 10.1101/2022.03.07.483347

Figure Lengend Snippet: [ 3 H]UCB-J, but not [ 3 H]Ro15-4513 or [ 3 H]flumazenil binding in Lhx6-Cre;Erbb4 F/F mice is decreased across the hippocampus. (A) [ 3 H]UCB-J showed a significant decrease in synaptic density in Lhx6-Cre;Erbb4 F/F mice (n=11) compared to control animals (n=9) across all hippocampal ROIs (mixed effects analysis; main effect of genotype: F (1,18) =7.27, p =0.02, ). (B) [ 3 H]Ro15-4513 (n=10-11/group) and (C) [ 3 H]flumazenil (n=9-10/group) binding did not differ by genotype. dHip CA1: dorsal hippocampus CA1; mHip CA3: middle hippocampus CA3; mHip CA1/2: middle hippocampus CA1/CA2; vHip CA3: ventral hippocampus CA3; dHip DG: dorsal hippocampus dentate gyrus; mHip DG: dentate gyrus.

Article Snippet: To quantify density of α5GABA A R – sections were incubated for 60 minutes at room temperature in 2nM [ 3 H]Ro15-4513 (Perkin Elmer, NET925250UC), or in 2nM [ 3 H]Ro15-4513 with 10 µM bretazenil (Sigma, B6434) for nonspecific binding.

Techniques: Binding Assay

Journal: The Journal of Neuroscience

Article Title: Ligand-Gated Ion Channel Subunit Partnerships: GABA A Receptor α 6 Subunit Gene Inactivation Inhibits δ Subunit Expression

doi: 10.1523/JNEUROSCI.17-04-01350.1997

Figure Lengend Snippet: Autoradiographic analysis of GABAAreceptor binding sites in wild-type (+/+) and α6 −/− mice. A, Benzodiazepine sites labeled by 5 nm [3H]R0 15-4513 showing total and diazepam-insensitive binding in the presence of 100 μmdiazepam. B, GABAA receptor sites labeled by 20 nm [3H]muscimol, showing total binding. The nonspecific signal in the presence of 100 μm GABA was at the film background level. C, GABAAreceptor sites labeled by 20 nm [3H]SR 95531, showing total binding. The nonspecific binding signal in the presence of 100 μm GABA was similar in wild-type and −/− brains (data not shown). Similar distinct pharmacological profiles were observed between the wild-type and α6 −/− brains in each of seven pairs of adult mice studied. D, E,In situ hybridization x-ray film autoradiographs of adult mouse brains hybridized with α4 (D) and δ-specific (E) 35S-labeled oligonucleotide probes. Wild-type (+/+) brains are on theleft; α6 −/− brains are on theright. No differences can be seen in subunit mRNA levels between +/+ and −/− brains. Note also the very similar pattern of α4 and δ gene expression in the forebrain/thalamus regions, and the correlation with the distribution of [3H]muscimol (B). Cbgr, Cerebellar granule cells; CP, caudate-putamen;Ctx, cerebral cortex; Gr, cerebellar granule cell layer; H, hippocampus; IC, inferior colliculus; OB, olfactory bulb;T, thalamus.

Article Snippet: For the benzodiazepine (BZ) site, [ 3 H]Ro 15-4513 (5 n m , Du Pont de Nemours, NEN Division, Dreieich, Germany) was used with and without 100 μ m diazepam (Orion, Espoo, Finland) for a 60 min incubation at 0°C, followed by three 30 sec washes, a dip in distilled water, and rapid drying.

Techniques: Binding Assay, Labeling, In Situ Hybridization, Gene Expression