rnaseiii Search Results


rnaseiii  (New England Biolabs)
98
New England Biolabs rnaseiii
Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant <t>RNaseIII</t> (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.
Rnaseiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/RNase+I/pmc06468493-135-57-58
Average 98 stars, based on 1 article reviews
rnaseiii - by Bioz Stars, 2026-09
98/100 stars
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anti-drosha antibody  (TargetMol)
N/A
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shortcut rnaseiii  (New England Biolabs)
96
New England Biolabs shortcut rnaseiii
Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant <t>RNaseIII</t> (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.
Shortcut Rnaseiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/ShortCut+RNase+III/bio_rxiv__2021__08__09__454968-142-21-23
Average 96 stars, based on 1 article reviews
shortcut rnaseiii - by Bioz Stars, 2026-09
96/100 stars
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primers  (ATCC)
99
ATCC primers
Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant <t>RNaseIII</t> (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.
Primers, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/Helicobacter+pylori+(Marshall+et+al%2E)+Goodwin+et+al/bio_rxiv__2020__02__15__950279-200-15-30
Average 99 stars, based on 1 article reviews
primers - by Bioz Stars, 2026-09
99/100 stars
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rnaseh  (New England Biolabs)
99
New England Biolabs rnaseh
Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant <t>RNaseIII</t> (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.
Rnaseh, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/RNase+H/pmc08055027-147-11-12
Average 99 stars, based on 1 article reviews
rnaseh - by Bioz Stars, 2026-09
99/100 stars
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hsirna  (Xeragon Inc)
90
Xeragon Inc hsirna
Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant <t>RNaseIII</t> (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.
Hsirna, supplied by Xeragon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/hsirna/us07700758-418-26-53
Average 90 stars, based on 1 article reviews
hsirna - by Bioz Stars, 2026-09
90/100 stars
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dicer-like (dcl) proteins  (Millar Inc)
90
Millar Inc dicer-like (dcl) proteins
Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant <t>RNaseIII</t> (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.
Dicer Like (Dcl) Proteins, supplied by Millar Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnaseiii/dicer+like++dcl++proteins/pm19802639-22-20-26
Average 90 stars, based on 1 article reviews
dicer-like (dcl) proteins - by Bioz Stars, 2026-09
90/100 stars
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recombinant drosha antibody  (Aladdin Scientific Corporation)
N/A
Rabbit anti Human Drosha Antibody, Recombinant, could be used for WB and so on.ApplicationWB: 1/500-1/1000Protein FunctionRibonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the
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drosha polyclonal antibody  (Elabscience)
N/A
This gene encodes a ribonuclease RNase III double stranded RNA specific ribonuclease and subunit of the microprocessor protein complex which catalyzes the initial processing step of microRNA miRNA synthesis The encoded protein cleaves the stem
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Image Search Results


Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant RNaseIII (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.

Journal: Nucleic Acids Research

Article Title: Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks

doi: 10.1093/nar/gkz024

Figure Lengend Snippet: Formation and p-Dicer-dependent turnover of dsRNA at DSBs. ( A ) ChIP analysis of GFP-RNaseH1 occupancy at DS1 using site-specific primers. ( B ) Quantitative real-time PCR (qRT-PCT) of DNA immunopurified from DNA–RNA hybrids (DRIP) or upon incubation with recombinant RNaseH at DS1 using S9.6 hybridoma supernatant and region-specific primers. ( C ) qRT-PCR analysis of transcripts associated with CTD Y1P and immunopurification (mNET-IP). Values were normalized to data in absence of GFP-RNaseH1. (A–E) Asterisk, P -value < 0.05, two-tailed t -test. Error bar: mean ± SEM, n = 3. ( D, E ) Imaging and RGB quantitation of CTD Y1P and p-Dicer (p-DCR-1). White box, 2.5× zoom; n , number of cells with shown phenotype in %. ( F ) qRT-PCR of cDNA after J2 immunoselection with (J2 RIP) and reverse transcription with forward (upper panel)- and reverse (lower panel)-oriented primers spanning a region up to 1000 nts distant from DS1 in presence (J2+) or absence (J2-) of 4OHT, or upon preincubation of lysed material with recombinant RNaseIII (J2+ RNaseIII) prior to J2 RIP. J2+ values were set to 1. Asterisk, p-value <0.05, two-tailed t-test. Error bar: mean ±SEM, n = 3. ( G ) Autoradiograph detecting J2 immuno-selected (RNA IP) or total (IN) RNA or pBR322 MspI digest (M) upon end-labeling and PAGE separation. AU, arbitrary units. ( H ) as in (D), but preincubated with Leptomycin B (LMB) and stained with J2. Representative images are shown.

Article Snippet: Cells were permeabilized with PBS/0.3% Tween-20 (10 min, RT), washed 1× in PBS and incubated for 20 min at RT with either BSA (Sigma, 0.2 μg/ml final conc., diluted in PBS containing 0.02 mM NaOAc and 0.2 mM Tris), RNaseA (Sigma, 0.2 μg/ml final conc., diluted in PBS containing 0.02 mM NaOAc and 0.2 mM Tris) or RNaseIII (NEB, 2U final conc., diluted in RNase-free H 2 O containing 1× commercial reaction buffer (NEB) prior to fixation.

Techniques: Real-time Polymerase Chain Reaction, Incubation, Recombinant, Quantitative RT-PCR, Immu-Puri, Two Tailed Test, Imaging, Quantitation Assay, Autoradiography, End Labeling, Staining