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Image Search Results
Journal: Methods in enzymology
Article Title: Radical SAM-Mediated Methylation of Ribosomal RNA
doi: 10.1016/bs.mie.2015.03.002
Figure Lengend Snippet: MALDI-TOF analysis of Cfr methylation in vivo. Fragment C2480–C2520 of E. coli 23S rRNA, isolated from BW2113/ΔrlmN strain transformed with pZA-Cfr plasmid, was digested with RNase T1 and fragments were analyzed by MALDI-TOF. Masses of the expected fragments from RNase T1 digestion, based on known nucleotide modifications, are shown in the inset. There is a slight shift in observed m/z values in comparison to predicted values due to difference in calibration of the instrument. m2A is 2-methyladenosine, m8A is 8-methyladenosine, and Ψ is pseudouridine.
Article Snippet: The RNA is then digested with 500 units of
Techniques: Methylation, In Vivo, Isolation, Transformation Assay, Plasmid Preparation, Comparison