rna extraction Search Results


minibest universal rna extraction kit  (TaKaRa)
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TaKaRa minibest universal rna extraction kit
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mgieasy rna directional library prep kit  (Complete Genomics Inc)
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Complete Genomics Inc mgieasy rna directional library prep kit
Mgieasy Rna Directional Library Prep Kit, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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europe cat favre 96004 austria  (Favorgen Biotech)
96
Favorgen Biotech europe cat favre 96004 austria
Europe Cat Favre 96004 Austria, supplied by Favorgen Biotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minibest viral rna dna extraction kit ver 5 0  (TaKaRa)
96
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minibest plant rna extraction kit  (TaKaRa)
96
TaKaRa minibest plant rna extraction kit
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virus rna extraction kit  (Vazyme Biotech Co)
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Vazyme Biotech Co virus rna extraction kit
Virus Rna Extraction Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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extractor labturbo 48 compact system  (LabTurbo Biotech)
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LabTurbo Biotech extractor labturbo 48 compact system
Extractor Labturbo 48 Compact System, supplied by LabTurbo Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vamne virus dna rna extraction kit 3 0  (Vazyme Biotech Co)
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Vazyme Biotech Co vamne virus dna rna extraction kit 3 0
Vamne Virus Dna Rna Extraction Kit 3 0, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna extraction  (Zymo Research)
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Zymo Research rna extraction
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monarch mag viral dna rna extraction kit  (New England Biolabs)
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New England Biolabs monarch mag viral dna rna extraction kit
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exosomes  (Novus Biologicals)
92
Novus Biologicals exosomes
Fig. 1 <t>Exosomes</t> derived from sorafenib-resistant Huh-7 cells induce an enhanced resistance to sorafenib in HCC cells. (A) Sorafenib-resistant cell line, Huh-7-SR, was established and confirmed by the IC50 value. (B) Co-culture systems were established to investigate the effects of sorafenib-resistant HCC cells on regular HCC cells. The group with additional GW4869 served as a negative control. (C) Cell viabilities of HCC cells co-cultured with Huh-7 or Huh- 7-SR cells under sorafenib stress were evaluated via CCK-8 assays. (D) Assessment of cell proliferation using colony formation assay in Huh-7 and Hep3B cells with the indicated co-culture model. (E) Concentration and size of exosome were measured by NTA. Exosomes were isolated from the supernatant of the culture medium of Huh-7-SR (Exo-SR) and Huh-7 (Exo-Norm) cells by ultracentrifugation. (F) The iconic cup-shaped double-layer closed vesicle structure of isolated exosome was confirmed by TEM. (G) Western blotting analysis of exosomal markers, including CD9, CD63, CD81, LAMP2, TSG101 and Calnexin. An exosome standard <t>from</t> <t>HEK293</t> cell line was analyzed as a positive control, while Huh-7-SR cell lysate was performed as a negative control. (H) The fluorescent signal of PKH67 labeled Exo-SR was captured in Huh-7 and Hep3B cells. (I) CCK-8 assays were performed to assess the cell viability of Huh-7 and Hep3B cells treated with sorafenib (6 µM) while co-cultured with the indicated exosomes. (J) Colony formation assays were performed to assess the cell proliferation of Huh-7 and Hep3B cells. The data are presented as the mean ± SD of at least three independent experiments. ***P < 0.001
Exosomes, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1 Exosomes derived from sorafenib-resistant Huh-7 cells induce an enhanced resistance to sorafenib in HCC cells. (A) Sorafenib-resistant cell line, Huh-7-SR, was established and confirmed by the IC50 value. (B) Co-culture systems were established to investigate the effects of sorafenib-resistant HCC cells on regular HCC cells. The group with additional GW4869 served as a negative control. (C) Cell viabilities of HCC cells co-cultured with Huh-7 or Huh- 7-SR cells under sorafenib stress were evaluated via CCK-8 assays. (D) Assessment of cell proliferation using colony formation assay in Huh-7 and Hep3B cells with the indicated co-culture model. (E) Concentration and size of exosome were measured by NTA. Exosomes were isolated from the supernatant of the culture medium of Huh-7-SR (Exo-SR) and Huh-7 (Exo-Norm) cells by ultracentrifugation. (F) The iconic cup-shaped double-layer closed vesicle structure of isolated exosome was confirmed by TEM. (G) Western blotting analysis of exosomal markers, including CD9, CD63, CD81, LAMP2, TSG101 and Calnexin. An exosome standard from HEK293 cell line was analyzed as a positive control, while Huh-7-SR cell lysate was performed as a negative control. (H) The fluorescent signal of PKH67 labeled Exo-SR was captured in Huh-7 and Hep3B cells. (I) CCK-8 assays were performed to assess the cell viability of Huh-7 and Hep3B cells treated with sorafenib (6 µM) while co-cultured with the indicated exosomes. (J) Colony formation assays were performed to assess the cell proliferation of Huh-7 and Hep3B cells. The data are presented as the mean ± SD of at least three independent experiments. ***P < 0.001

Journal: Journal of nanobiotechnology

Article Title: Exosome-derived circUPF2 enhances resistance to targeted therapy by redeploying ferroptosis sensitivity in hepatocellular carcinoma.

doi: 10.1186/s12951-024-02582-6

Figure Lengend Snippet: Fig. 1 Exosomes derived from sorafenib-resistant Huh-7 cells induce an enhanced resistance to sorafenib in HCC cells. (A) Sorafenib-resistant cell line, Huh-7-SR, was established and confirmed by the IC50 value. (B) Co-culture systems were established to investigate the effects of sorafenib-resistant HCC cells on regular HCC cells. The group with additional GW4869 served as a negative control. (C) Cell viabilities of HCC cells co-cultured with Huh-7 or Huh- 7-SR cells under sorafenib stress were evaluated via CCK-8 assays. (D) Assessment of cell proliferation using colony formation assay in Huh-7 and Hep3B cells with the indicated co-culture model. (E) Concentration and size of exosome were measured by NTA. Exosomes were isolated from the supernatant of the culture medium of Huh-7-SR (Exo-SR) and Huh-7 (Exo-Norm) cells by ultracentrifugation. (F) The iconic cup-shaped double-layer closed vesicle structure of isolated exosome was confirmed by TEM. (G) Western blotting analysis of exosomal markers, including CD9, CD63, CD81, LAMP2, TSG101 and Calnexin. An exosome standard from HEK293 cell line was analyzed as a positive control, while Huh-7-SR cell lysate was performed as a negative control. (H) The fluorescent signal of PKH67 labeled Exo-SR was captured in Huh-7 and Hep3B cells. (I) CCK-8 assays were performed to assess the cell viability of Huh-7 and Hep3B cells treated with sorafenib (6 µM) while co-cultured with the indicated exosomes. (J) Colony formation assays were performed to assess the cell proliferation of Huh-7 and Hep3B cells. The data are presented as the mean ± SD of at least three independent experiments. ***P < 0.001

Article Snippet: A positive control was conducted using a standard sample of exosomes derived from HEK293 cells (Novus, USA), while a negative control was performed using Huh-7-SR cell lysate.

Techniques: Derivative Assay, Co-Culture Assay, Negative Control, Cell Culture, CCK-8 Assay, Colony Assay, Concentration Assay, Isolation, Western Blot, Positive Control, Labeling