Journal: Cell Death & Disease

Article Title: Loss of RhoA in microglia disables glycolytic adaptation and impairs spinal cord injury recovery through Arhgap25/HIF-1α pathway

doi: 10.1038/s41419-025-07947-9

Figure Lengend Snippet: A Changes of RhoA mRNA expression in injured spinal cord obtained from different GEO databases. B Temporal changes of RhoA protein level in spinal cord following SCI ( n = 5). C Major cell type classification in the GSE196928 dataset. D Distribution map of RhoA expression. E RhoA expression levels across different cell types. F Immunostaining shows the RhoA expression in microglia (IBA-1 + ) at different stages after SCI ( n = 3). Data are presented as mean ± SD. Statistical significance: ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.001 (vs. sham group).

Article Snippet: RhoA flox/flox and Cx3cr1 Cre mice (provided by Cyagen, Suzhou, China) were intercrossed to obtain RhoA cKO mice with genotype of RhoA flox/flox ; Cx3cr1 Cre , as previously described [ ].

Techniques: Expressing, Immunostaining

Journal: Cell Death & Disease

Article Title: Loss of RhoA in microglia disables glycolytic adaptation and impairs spinal cord injury recovery through Arhgap25/HIF-1α pathway

doi: 10.1038/s41419-025-07947-9

Figure Lengend Snippet: A t-SNE and unsupervised clustering of all cells based on biomarkers. B GSEA analysis highlighting differences in microglial subsets compared to other cell types. C High-resolution reclustering of microglia into ten distinct clusters. D Expression of key markers identified in the reclustered microglia. E Pseudotime trajectory analysis of microglial cells. F Visualization of ten microglial subclusters, each represented by a distinct color. Microglial subsets with significant differences in oxidative phosphorylation pathways ( G subcluster 0, 2, 3, 4, 7) and glycolysis pathways ( H subcluster 2, 3, 4, 7) identified through GSEA analysis. I CellChat analysis showing interactions between glycolysis-differentiated microglia (subcluster 2, 3, 4, 7) and other cell types. J PCA of bulk mRNA transcriptomics in primary microglial cells. K Heatmap illustrating the results of mRNA sequencing analysis. GSEA analysis of glycolysis-related ( L ) and oxidative phosphorylation-related ( M ) differences between flox and cKO groups. N PCA of metabolomic profiling data. O , P Comparative analysis of glycolysis and TCA cycle metabolic changes between cKO and flox groups. RhoA deletion decreases ATP production ( Q ), accompanied by reduced ECAR ( R ), OCR ( S ), and glucose consumption in the culture medium ( T ). Data are presented as mean ± SD; each dot represents an individual mouse. Statistical significance: *** p < 0.001.

Article Snippet: RhoA flox/flox and Cx3cr1 Cre mice (provided by Cyagen, Suzhou, China) were intercrossed to obtain RhoA cKO mice with genotype of RhoA flox/flox ; Cx3cr1 Cre , as previously described [ ].

Techniques: Expressing, Phospho-proteomics, Sequencing