Journal: Blood Advances

Article Title: IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells

doi: 10.1182/bloodadvances.2016002352

Figure Lengend Snippet: Cytokines used for in vitro culture of ILC1, ILC2, and ILC3

Article Snippet: For intracellular staining experiments, cells were plated at a density of 1000 to 2000 cells per well. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Cytokine Supplier Catalog number Concentration (ng/mL) Recombinant human IL-2 R & D Systems 202-IL-050 500 Recombinant human IL-23 R & D Systems 1290-IL-010 50 Recombinant human IL-12 R & D Systems 219-IL-025 50 Recombinant human IL-33 MedImmune N/A 500 Recombinant human IL-17E Peprotech AF-200-24 500 Recombinant human IL-1 β R & D Systems 201-LB-025 500 Recombinant human IL-7 R & D Systems 207-IL-005 10 Recombinant human TSLP R & D Systems 1398-TS-010 500 Open in a separate window Cytokines used for in vitro culture of ILC1, ILC2, and ILC3 Flow cytometry Cells were centrifuged for 10 minutes at 400 g , and supernatants collected and stored at −80°C.

Techniques: In Vitro, Concentration Assay, Recombinant

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: IL-17A promotes fatty acid uptake through the IL-17A/IL-17RA/p-STAT3/FABP4 axis to fuel ovarian cancer growth in an adipocyte-rich microenvironment

doi: 10.1007/s00262-019-02445-2

Figure Lengend Snippet: Relationship between IL-17A protein level and clinical outcomes in clinical ovarian cancer settings

Article Snippet: To examine the effect of recombinant human IL-17A (rhIL-17A, PeproTech Cat# 200-17) on the mRNA level of FABP4 in OvCa cells, OVCAR3 and A2780 cells were seeded in six-well plates at 5 × 10 5 cells per well, and then they were treated with rhIL-17A (0, 1, 10 or 50 ng/ml) for 24 h. Then the cells were harvested and homogenized for RNA extraction using TRIzol Reagent (TIANGEN BIOTECH Cat# DP430). cDNA was synthesized from the total RNA (2 μg) in a 20 μl reaction using a reverse transcription system (TIANGEN BIOTECH Cat# KR103-04).

Techniques: