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Image Search Results
Journal: Nucleic Acids Research
Article Title: Acute hepatotoxicity of 2′ fluoro-modified 5–10–5 gapmer phosphorothioate oligonucleotides in mice correlates with intracellular protein binding and the loss of DBHS proteins
doi: 10.1093/nar/gky060
Figure Lengend Snippet: Time course of mRNA alterations and stress phenotypes induced by 2′ F PS-ASO (ION-404130) in vivo . ( A–F) Mice were treated with a single 400 mg/kg 2′ F PS-ASO (ION-404130) or with saline and indicated parameters were quantified at 24, 48, 72 and 96 h after dosing. N = 3 per group. The error bars indicate data acquired from three individual animals. S-saline, and F-2′ F PS-ASO (ION-404130). ( A ) qRT-PCR quantification of on-target Pten mRNA levels in liver. ( B ) Plasma levels of ALT and AST. ( C ) qRT-PCR of mRNA transcripts Cdkn1a/p21 and Gadd45a . ( D ) qRT-PCR of liver mRNA levels of anti-apoptosis markers Mcl1 and Bcl-xl . ( E ) qRT-PCR of liver mRNA levels of necrosis markers that are up-regulated ( Map3k6 and Cd68 ) or down-regulated ( Lgr5 and Rhbg ) by 2′ F ASO. ( F ) Representative images of H&E staining and active caspase 3 staining of liver sections.
Article Snippet: Primer-probe sets for mouse Cdkn1a (Mm01303209_m1), mouse Gadd45a (Mm00432802_m1), mouse Mcl1 (Mm01257351_g1), mouse Bcl-xl (Mm00437783_m1), mouse Map3k6 (Mm00522235_m1), mouse Cd68 (Mm03047343_m1), mouse Lgr5 (Mm00438890_m1), mouse Rhbg (
Techniques: In Vivo, Saline, Quantitative RT-PCR, Clinical Proteomics, Staining