Journal: Molecules

Article Title: An Alternative HIV-1 Non-Nucleoside Reverse Transcriptase Inhibition Mechanism: Targeting the p51 Subunit

doi: 10.3390/molecules25245902

Figure Lengend Snippet: The two compounds that inhibited HIV-1 reverse transcriptase (RT) function. ( A ) RT inhibition was demonstrated via the best-fit curves (using ΔCt) of compound 1 (NSC48443), compound 2 (NSC127133), and nevirapine (as a positive control). Chemical structures of the compounds are shown on the top. ( B ) Densitometry analysis is shown with various concentrations of compounds 1 and 2 (1 µM to 1000 µM), nevirapine (0.01 µM to 10 µM), and the control. Asterisks depict p -values of statistical tests of the mean difference against the untreated control (with RT and no inhibitors added), p < 0.05 (*) and p < 0.01 (**). All of the experiments were performed in triplicates. The densitometric values were estimated and normalized against those of the control, using Fiji software .

Article Snippet: Forty NCI Diversity Set V chemical compounds from the National Cancer Institute (NCI) Developmental Therapeutic Program’s Open Compound Repository, National Institutes of Health (NIH) ( http://dtp.cancer.gov ) and Nevirapine (MedChemExpress LLC, Cat No. HY-10570) were reconstituted in dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Reverse Transcription, Inhibition, Positive Control, Control, Software

Journal: Molecules

Article Title: An Alternative HIV-1 Non-Nucleoside Reverse Transcriptase Inhibition Mechanism: Targeting the p51 Subunit

doi: 10.3390/molecules25245902

Figure Lengend Snippet: Inhibition analysis of individual RT subunits p66 and p51 by the identified compounds. ( A ) Results of post-cDNA synthesis analysis when treated with the two compounds. The control was performed using DMSO with RT. ( B ) RT-qPCR results of individual RT p51 and p66 subunits with no inhibitors added. ( C ) Densitometry analysis of the various concentrations of compounds 1 and 2 (1 µM to 1000 µM) and nevirapine (0.01 µM to 10 µM) in the p66 subunit alone. Asterisks depict p -values of statistical tests of the mean difference against the untreated control (with RT and no inhibitors added), p < 0.05 (*) and p < 0.01 (**). All of the experiments were performed in triplicates to quantify the GAPDH gene products on 2% agar gels. All of the band sizes were estimated ~120 bp using GelApp . The p51 and p66 only gels were performed on the same gel but separate from each other. The densitometric values were estimated and normalized against those of the control, using the Fiji software .

Article Snippet: Forty NCI Diversity Set V chemical compounds from the National Cancer Institute (NCI) Developmental Therapeutic Program’s Open Compound Repository, National Institutes of Health (NIH) ( http://dtp.cancer.gov ) and Nevirapine (MedChemExpress LLC, Cat No. HY-10570) were reconstituted in dimethyl sulfoxide (DMSO) and stored at 4 °C.

Techniques: Inhibition, Control, Quantitative RT-PCR, Software