Journal: Scientific Reports

Article Title: CD38 modulates cytokine secretion by NK cells through the Sirt1/NF-κB pathway, suppressing immune surveillance in colorectal cancer

doi: 10.1038/s41598-024-79008-8

Figure Lengend Snippet: The effect of CD38-mediated Sirt1/NF-κB signaling on cytokine secretion and the resultant modulation of CD4 + T-cell differentiation. Expression levels of NF-κB and acetyl-NF-κB proteins in CD38 + NK cells treated with RSV (Sirt1 activator) or DMSO ( A ) and quantitation for NF-κB ( B ) and acetyl-NF-κB ( C ). Expression levels of NF-κB and acetyl-NF-κB proteins in CD38 + NK cells treated with NAM (Sirt1 inhibitor) or DMSO ( D ) and quantitation for NF-κB ( E ) and acetyl-NF-κB ( F ). ( G ) Acetyl-NF-κB expression was compared with NF-κB expression. ( H ) Cytokine levels in the culture supernatant of CD38 + NK cells treated with PDTC (NF-κB inhibitor) or PBS. (I). CD4 + T cells were cocultured with CD38 + NK cells pretreated with PDTC or PBS. ( J ). Proportions of Tregs, Th1, Th2, and Th17 cells and the ratios of Th17/Treg and Th1/Th2 cells among CD4 + T cells cocultured with CD38 + NK cells that were pretreated with PDTC or PBS. CD38 + NK (HC): CD38 + NK cells isolated from the blood of healthy controls; CD38 + NK (CRC): CD38 + NK cells isolated from the blood of CRC patients; CRC cells: colorectal cancer; RSV: resveratrol; NAM: nicotinamide; DMSO: dimethyl sulfoxide. ns: not statistically significant; *: P < 0.05, **: P < 0.01 and ***: P < 0.001.

Article Snippet: CD38 + NK cells were also pretreated with resveratrol (RSV, MCE, USA), an activator of Sirt1, at a concentration of 20 μM; nicotinamide (NAM, MCE), an inhibitor of Sirt1, at a concentration of 100 μM; or pyrrolidinedithiocarbamate ammonium (PDTC, MCE), an inhibitor of NF-κB, at a concentration of 25 μM.

Techniques: Cell Differentiation, Expressing, Quantitation Assay, Isolation

Journal: Scientific Reports

Article Title: BRCA1 inhibits AR–mediated proliferation of breast cancer cells through the activation of SIRT1

doi: 10.1038/srep22034

Figure Lengend Snippet: ( A ) Cell viability was measured by MTT assay following treatment of MCF-7, T47D, and SK-BR-3 cells with resveratrol at indicated concentrations for 48 h. ( B ) Cell viability was measured by MTT assay following treatment with resveratrol at the indicated concentrations, for 24, 36, 48, or 60 h. ( C ) Cell apoptosis was detected by flow cytometry analysis after 40 μM resveratrol treatment. ( D , E ) SIRT1, mTOR, AR, and Skp2/B protein levels were determined by western blotting and analyzed by grayscale software following treatment with 40 μM resveratrol for 48 h, and normalized to GAPDH expression. ( F , G ) SIRT1 and AR protein levels were determined by western blotting and analyzed by grayscale software following treatment with 40 μM resveratrol and/or transfection with siSIRT1 for 48 h, and normalized to GAPDH expression. Data in are the mean of three independent experiments. Res: resveratrol. * P < 0.05, as compared with untreated cells.

Article Snippet: Resveratrol (Selleck Chemicals, Houston, TX, USA) was dissolved in dimethylsulfoxide (DMSO, Sigma) and stored at −80 °C as a 1 mM stock solution.

Techniques: MTT Assay, Flow Cytometry, Western Blot, Software, Expressing, Transfection

Journal: Scientific Reports

Article Title: BRCA1 inhibits AR–mediated proliferation of breast cancer cells through the activation of SIRT1

doi: 10.1038/srep22034

Figure Lengend Snippet: ( A,B ) Total number of tumors after removal from the mice. ( C ) Tumor growth curve. MCF-7 cells xenograft mouse model was intraperitoneally injected with PBS, 100 μM resveratrol, or 200 μM resveratrol every group. Tumor growth was measured from day two after injecting tumor cells. The error bars represent the standard deviation (SD). ( D ) Tumor weight when the tumors were harvested. The data represent the mean ± SD.

Article Snippet: Resveratrol (Selleck Chemicals, Houston, TX, USA) was dissolved in dimethylsulfoxide (DMSO, Sigma) and stored at −80 °C as a 1 mM stock solution.

Techniques: Injection, Standard Deviation

Journal: Scientific Reports

Article Title: BRCA1 inhibits AR–mediated proliferation of breast cancer cells through the activation of SIRT1

doi: 10.1038/srep22034

Figure Lengend Snippet: ( A,B ) Representative immunohistochemical staining of BRCA1 and SIRT high- ( A ) or low-expression ( B ) in the breast cancer patient tissues. ( C ) OS in high and low SIRT1-expressing tumor tissues. ( D ) OS in AR high-/SIRT1 low- or AR low-/SIRT1 high-expressing tumor tissues. ( E ) BRCA1 and SIRT1 expression from TCGA database of 1210 patients’ tissues. ( F ) SIRT1 mRNA levels from TCGA database including 111 normal adjacent tissues, 1092 primary tumor tissues, and 7 metastatic tumor tissues. ( G ) A model showing a BRCA1–SIRT1–AR axis signaling pathway involved in tumor cell survival and proliferation. Our data indicate that resveratrol treatment induces the activity of SIRT1, which inhibits AR and potentially other factors leading to reduced cell proliferation and increased apoptosis.

Article Snippet: Resveratrol (Selleck Chemicals, Houston, TX, USA) was dissolved in dimethylsulfoxide (DMSO, Sigma) and stored at −80 °C as a 1 mM stock solution.

Techniques: Immunohistochemical staining, Staining, Expressing, Activity Assay

Journal: Diabetes

Article Title: Altered MAPK Signaling in Progressive Deterioration of Endothelial Function in Diabetic Mice

doi: 10.2337/db12-0559

Figure Lengend Snippet: Shear stress (20 dynes/cm 2 )–induced dilation in mesenteric arteries of 9M Lepr db−/− and Lepr db+/− mice, in control and in the presence of resveratrol. n = 6–8 per group. *Significant difference between groups. PD, passive diameter.

Article Snippet: In separate experiments, SSID was assessed in vessels of 9M mice in control and in the presence of resveratrol (100 nmol/L, Erk activator; 3,4',5-trihydroxystilbene; LKT Laboratories) and resveratrol plus one of following inhibitors: SB203580, SP600125, and VAS2870 without or with additional U0126 (1 μmol/L, inhibitor of Erk).

Techniques: Shear, Control

Journal: Diabetes

Article Title: Altered MAPK Signaling in Progressive Deterioration of Endothelial Function in Diabetic Mice

doi: 10.2337/db12-0559

Figure Lengend Snippet: Shear stress (20 dynes/cm 2 )–induced dilation in mesenteric arteries of 9M Lepr db−/− mice in control and in the presence (+) or absence (─) of resveratrol, SB203580, SP600125, VAS2870, and U0126, respectively. n = 8 per group. *Significant difference between groups. PD, passive diameter.

Article Snippet: In separate experiments, SSID was assessed in vessels of 9M mice in control and in the presence of resveratrol (100 nmol/L, Erk activator; 3,4',5-trihydroxystilbene; LKT Laboratories) and resveratrol plus one of following inhibitors: SB203580, SP600125, and VAS2870 without or with additional U0126 (1 μmol/L, inhibitor of Erk).

Techniques: Shear, Control

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Summary of compound characteristics and relevant previously reported effects.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: In Vitro

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Quantification of platelet-derived reactive oxygen species and platelet mitochondrial density in the presence of polyphenols. Comparison of (A) platelet cellular reactive oxygen species (quantified by fluorescence intensity of permeant reagent 2′,7′-dichlorofluorescein (DCF) diacetate at 500/550 nm excitation/emission;) and (B) platelet mitochondrial density (determined using a citrate synthase activity) in normoglycemic vs hyperglycemic conditions (5.6 mM vs 25 mM glucose in isolation buffer, respectively). Washed platelets were incubated with polyphenols (20 µM) for 1 hour prior to measurements. “−ve control” in A refers to N-acetyl cysteine (provided with a commercial assay kit). Results are shown as mean ± SEM, n = 4 with 3 technical repeats. EGCG, epigallocatechin gallate; hesp, hesperetin ns, nonsignificant; resv, resveratrol. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001 difference from control (washed platelets only) or dimethyl sulfoxide (DMSO) in black or gray, respectively. # P < .01; ### P < .001 difference from the corresponding condition at 5.6 mM.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: Derivative Assay, Comparison, Fluorescence, Activity Assay, Isolation, Incubation, Control

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Mitochondrial calcium flux in normoglycemic vs acute hyperglycemic conditions in the absence and presence of polyphenols. Healthy volunteers’ platelets were isolated in isolation media containing 5.6 mM vs 25 mM glucose in isolation buffer (representing normoglycemia and acute hyperglycemia, respectively). Mitochondrial calcium flux was measured kinetically by flow cytometry in platelets labeled with X-Rhod-1 calcium indicator dye for 1 hour activated with convulxin. (A) Different concentrations (25-100 ng/mL) of convulxin were tested, with 100 ng/mL chosen for future experiments based on changes in area under the curve (AUC). (B) Kinetic time series, obtained with FlowJo, for platelets activated with 100 ng/mL convulxin. Following incubation with X-Rhod-1 calcium indicator dye for 1 hour, platelets were incubated with 20 µM (C) resveratrol (resv), (D) hesperetin (hesp), or (E) epigallocatechin gallate (EGCG) for 1 hour prior to analysis. An equivalent concentration of dimethyl sulfoxide in samples with polyphenols was added to controls (ctl). (F) AUC was determined in normoglycemia vs acute hyperglycemia in the presence of polyphenols. Results are shown as mean ± SEM, (A and B) n = 3 to 5 and (C–F) n = 3. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001 difference from ctl.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: Isolation, Flow Cytometry, Labeling, Incubation, Concentration Assay

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Quantification of oxygen consumption rate (OCR) in normoglycemia and acute hyperglycemia in the presence of resveratrol (Resv). Mitochondrial stress test was performed on healthy volunteers’ isolated platelets in the presence and absence of Resv using Seahorse XF Analyzer to determine OCR. Healthy volunteers’ platelets were isolated in isolation media containing 5.6 mM vs 25 mM glucose in isolation buffer (representing normoglycemia and acute hyperglycemia, respectively). Isolated platelets were treated with Resv (20 µM) for 45 to 60 minutes prior to activation and analysis. (A) Once baseline (BL) OCR levels were determined, platelets were activated (Act) with 100 ng/mL convulxin, followed by subsequent injections of oligomycin [adenosine triphosphate (ATP) synthase inhibitor], carbonylcyanide-p-trifluoromethoxyphenylhydrazone (uncoupling agent), and rotenone and antimycin A (complex I and III inhibitors) to determine OCR profile. Agonist was not added to BL samples. From the OCR curve, (B) basal respiration, proton leak, maximal respiration, spare respiratory capacity, nonmitochondrial oxygen consumption, and ATP production were determined. Results are shown as mean ± SEM, n = 3 to 4. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001 difference.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: Isolation, Activation Assay

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Procoagulant platelet and fibrin fiber quantification in clots formed in the presence of resveratrol in normoglycemic vs acute hyperglycemic conditions. Healthy volunteers’ platelet-rich plasma (PRP) was diluted with saline containing no glucose, 5 mM (representative of normoglycemia), or 25 mM (representative of hyperglycemia) glucose. (A) Absolute procoagulant platelet number was determined by laser scanning confocal microscopy. (B) Fibrin fiber density was determined by laser scanning confocal microscopy. (C) Representative confocal images of procoagulant platelets (annexin V). (D) Representative confocal images of fibrin fibers (Alexa Fluor-488 fibrinogen). “PRP” refers to clots formed in the absence of resveratrol, and “Resveratrol” refers to clots formed in the presence of 20 µM of the compound. Dimethyl sulfoxide (“DMSO”) refers to clots formed in the presence of an equivalent concentration of this solvent in the “Resveratrol” sample as a control. Tissue factor was used to initiate clotting following 20-minute incubation with 0 to 5 mM glucose ± resveratrol. Results are shown as mean ± SEM, n = 4. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001 difference from PRP control or DMSO in black and gray, respectively. ## P < .01 difference from 0 mM PRP control. Scale bar represents 25 µm.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: Clinical Proteomics, Saline, Confocal Microscopy, Concentration Assay, Solvent, Control, Coagulation, Incubation

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Thrombus formation under shear flow in normoglycemia in the presence of resveratrol (Resv). Healthy volunteers’ platelets were isolated in isolation media containing 5 mM glucose (representing normoglycemia). Isolated platelets were treated with Resv (20 µM) or buffer (control) and stained with 0.5 mM DIOC6 for 15 minutes. Washed red blood cells were added to samples to a final hematocrit of 40%. Red blood cell–platelet mixture was perfused over 20 μg/mL of a collagen-coated chip using a microfluidic syringe pump and recorded for 5 minutes at 1500 s −1 . (A) Platelet deposition at 5-minute time point from when platelets first adhered to collagen strands was quantified from images. Representative image of platelet deposition in (B) control and (C) Resveratrol treated platelets. Results are shown as mean ± SEM, n = 5. ∗ P < .05 difference from control.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: Shear, Isolation, Control, Staining

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Plant-derived compounds normalize platelet bioenergetics and function in hyperglycemia

doi: 10.1016/j.rpth.2024.102548

Figure Lengend Snippet: Summary of effects of normoglycemia vs acute hyperglycemia ± polyphenols on platelet bioenergetics and clot structure.

Article Snippet: Platelets were incubated for 15 minutes with either 20 μM resveratrol (Tocris) or buffer and stained for 15 minutes with 0.5 mM DIOC6(3) (Thermo Fisher Scientific) to label the platelets.

Techniques: Activation Assay, Shear, Pore Size

Journal: Frontiers in Oncology

Article Title: Dietary Polyphenols, Resveratrol and Pterostilbene Exhibit Antitumor Activity on an HPV E6-Positive Cervical Cancer Model: An in vitro and in vivo Analysis

doi: 10.3389/fonc.2019.00352

Figure Lengend Snippet: Resveratrol and pterostilbene are cytotoxic to TC1 cells. (A) Brightfield analysis of TC1 cells untreated (Control) or treated with 10, 20, and 30 μM of resveratrol (Res) or pterostilbene (Pte). . (B) IC 50 values generated using a water soluble tetrazolium salt-1 (WST-1) assay after 72 h of exposure to resveratrol or pterostilbene shows that pterostilbene (Bi) is more cytotoxic than resveratrol (Bii) (IC 50 = 15.61 μM vs. IC 50 = 34.46 μM; p < 0.0001, n = 3). (Biii) Bar graph represents comparative data showing the difference in viable cells treated with resveratrol or pterostilbene at 20 or 40 μM (mean ± S.E.M.; * p < 0.05, n = 3).

Article Snippet: Four thousand TC-1 cells were plated on 96-well plates and cultured for 24 h. Resveratrol (Acros, #430075000) or pterostilbene (TCI, #P1924) were serially diluted from 5 to 100 μM in DMEM/F-12 supplemented with 1 × insulin-transferrin-selenium (ITS; Invitrogen).

Techniques: Control, Generated, WST-1 Assay

Journal: Frontiers in Oncology

Article Title: Dietary Polyphenols, Resveratrol and Pterostilbene Exhibit Antitumor Activity on an HPV E6-Positive Cervical Cancer Model: An in vitro and in vivo Analysis

doi: 10.3389/fonc.2019.00352

Figure Lengend Snippet: Viral oncoprotein E6 gets downregulated in TC1 cells treated with resveratrol or pterostilbene. (A) TC1 cells immunostained and probed for E6 oncoprotein (green) and counterstained with nuclear stain DAPI (blue). Cells treated for 48 h with resveratrol (30 μM) or pterostilbene (30 μM) shows pterostilbene has a more significant downregulation of E6 protein expression as compared to resveratrol and control. (B) Bar graph indicates the percent decrease in E6 expression in treated TC1 cells as compared to control untreated TC1 cells. The graphs represent data from three independent experiments (mean ± S.E.M.; * p < 0.05).

Article Snippet: Four thousand TC-1 cells were plated on 96-well plates and cultured for 24 h. Resveratrol (Acros, #430075000) or pterostilbene (TCI, #P1924) were serially diluted from 5 to 100 μM in DMEM/F-12 supplemented with 1 × insulin-transferrin-selenium (ITS; Invitrogen).

Techniques: Staining, Expressing, Control

Journal: Frontiers in Oncology

Article Title: Dietary Polyphenols, Resveratrol and Pterostilbene Exhibit Antitumor Activity on an HPV E6-Positive Cervical Cancer Model: An in vitro and in vivo Analysis

doi: 10.3389/fonc.2019.00352

Figure Lengend Snippet: Inhibition of tumor growth in E6+ tumor bearing mouse. (A) Schematic representation of mouse TC1 tumor in vivo tumor generation and subsequent treatment cycle. TC1 cells were injected subcutaneously in 15 mice followed by daily palpations. After 15–20 days, when the tumors became apparent mice were divided into three groups of five and treated intralesionally with either PBS (control), resveratrol or pterostilbene for 5 consecutive days. After 5 days the mice were sacrificed, and the tumor excised. (B) Tumors excised from the three representative groups, displayed for imaging with corresponding volume (ml). In both treatment group tumors show marked decrease in tumor size compared to control. (C) Bar graph shows percent reduction in tumor size in mice treated with resveratrol or pterostilbene in comparison to control tumor. Tumor size reduction is significant in the treated groups. The graphs represent data from 5 mice from each group (mean ± S.E.M.; * p < 0.05).

Article Snippet: Four thousand TC-1 cells were plated on 96-well plates and cultured for 24 h. Resveratrol (Acros, #430075000) or pterostilbene (TCI, #P1924) were serially diluted from 5 to 100 μM in DMEM/F-12 supplemented with 1 × insulin-transferrin-selenium (ITS; Invitrogen).

Techniques: Inhibition, In Vivo, Injection, Control, Imaging, Comparison

Journal: Frontiers in Oncology

Article Title: Dietary Polyphenols, Resveratrol and Pterostilbene Exhibit Antitumor Activity on an HPV E6-Positive Cervical Cancer Model: An in vitro and in vivo Analysis

doi: 10.3389/fonc.2019.00352

Figure Lengend Snippet: E6 expression is reduced in mouse tumors. (A) Tumor sections immunostained with E6 antibody shows decreased E6 protein (green) in mice treated with resveratrol or pterostilbene when compared to control untreated tumors. Sections were counterstained with DAPI (blue). Scale bar: 47.62 μm. (B) Graph indicates the significant reduction of E6 expression levels in resveratrol or pterostilbene treated tumors in comparison to control tumors sections (mean ± S.E.M.; * p < 0.05).

Article Snippet: Four thousand TC-1 cells were plated on 96-well plates and cultured for 24 h. Resveratrol (Acros, #430075000) or pterostilbene (TCI, #P1924) were serially diluted from 5 to 100 μM in DMEM/F-12 supplemented with 1 × insulin-transferrin-selenium (ITS; Invitrogen).

Techniques: Expressing, Control, Comparison

Journal: Frontiers in Oncology

Article Title: Dietary Polyphenols, Resveratrol and Pterostilbene Exhibit Antitumor Activity on an HPV E6-Positive Cervical Cancer Model: An in vitro and in vivo Analysis

doi: 10.3389/fonc.2019.00352

Figure Lengend Snippet: Upregulation of activated caspase 3 and downregulation of PCNA expression in mouse tumors. (A) Tumor sections immunostained with cleaved caspase 3 antibody shows elevated protein levels (green) in mice treated with pterostilbene when compared to control untreated tumors. Resveratrol treated tumors did not show any significant change in caspase 3 expression. Sections were counterstained with DAPI (blue). Scale bar: 47.62 μm. (B) Graph indicates the significant increase of Cleaved caspase 3 expression levels in pterostilbene treated tumors in comparison to control tumors sections (mean ± S.E.M.; * p < 0.0001). (C) Tumor sections immunostained with PCNA protein (green) and counterstained with nuclear stain DAPI (blue). Resveratrol treated tumors display a significant decrease in PCNA expression compared to control sections. Pterostilbene treated tumors show similar PCNA levels as control. Scale bar: 47.62 μm. (D) Quantitative analysis of PCNA expression shows a significant change in resveratrol treated tumor sections (mean ± S.E.M.; * p < 0.0004; The two treatment groups show significant differences in PCNA expression ( p < 0.0006).

Article Snippet: Four thousand TC-1 cells were plated on 96-well plates and cultured for 24 h. Resveratrol (Acros, #430075000) or pterostilbene (TCI, #P1924) were serially diluted from 5 to 100 μM in DMEM/F-12 supplemented with 1 × insulin-transferrin-selenium (ITS; Invitrogen).

Techniques: Expressing, Control, Comparison, Staining

Journal: Frontiers in Oncology

Article Title: Dietary Polyphenols, Resveratrol and Pterostilbene Exhibit Antitumor Activity on an HPV E6-Positive Cervical Cancer Model: An in vitro and in vivo Analysis

doi: 10.3389/fonc.2019.00352

Figure Lengend Snippet: Downregulation of VEGF expression in mouse tumors. (A) Tumor sections immunostained with VEGF protein (green) and counterstained with nuclear stain DAPI (blue). Both resveratrol and pterostilbene treated tumors display a significant decrease in VEGF expression compared to control sections. Scale bar: 47.62 μm. (B) Quantitative analysis of VEGF expression shows a significant change in treated tumor sections (mean ± S.E.M.; * p < 0.05).

Article Snippet: Four thousand TC-1 cells were plated on 96-well plates and cultured for 24 h. Resveratrol (Acros, #430075000) or pterostilbene (TCI, #P1924) were serially diluted from 5 to 100 μM in DMEM/F-12 supplemented with 1 × insulin-transferrin-selenium (ITS; Invitrogen).

Techniques: Expressing, Staining, Control

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: Structure of trans-resveratrol.

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques:

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: %Entrapment efficiencies of DMC, Res pluronic micelles.

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques:

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: HPLC chromatograms blank pluronic nanomicelles [A]; mixture of DMC (10 mg/ml and resveratrol (10 mg/ml) under UV detector at 306 nm [B] and under fluorescence detector (excitation wavelength 420 nm; emission wavelength 470 nm) [C].

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques: Fluorescence

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: LOD and LOQ (µg/ml) of the analytes.

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques:

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: Linear ranges and correlation coefficients of the calibration curves.

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques: Concentration Assay

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: Recovery (%) of Resveratrol in working standard solution (QC sample).

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques:

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: RSD (%) of between-run analysis and within-run analysis of DMC and resveratrol.

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques:

Journal: MethodsX

Article Title: Validated HPLC method for simultaneous quantitative determination of dimethylcurcumin and resveratrol in pluronic-F127 nanomicelles: Formulation with enhanced anticancer efficacy

doi: 10.1016/j.mex.2023.102457

Figure Lengend Snippet: The IC 50 values of DMC, resveratrol and doxorubicin against MCF-7 and MDA-MB 231.

Article Snippet: Resveratrol was purchased from Carbosynth Limited, Compton, Berkshire, UK.

Techniques: