Journal: Retina (Philadelphia, Pa.)

Article Title: LEBER CONGENITAL AMAUROSIS DUE TO CEP290 MUTATIONS—SEVERE VISION IMPAIRMENT WITH A HIGH UNMET MEDICAL NEED

doi: 10.1097/IAE.0000000000003133

Figure Lengend Snippet: Clinical Trials Investigating Gene Therapy for LCA Where the Drug is Still Currently in Development *

Article Snippet: LCA2 , AAV 2/5 -OPTIRPE65 , MeiraGTx UK II Ltd. , 1/2 , NCT02781480 , 15 , April 2016 , Completed.

Techniques: Clinical Proteomics

Journal: bioRxiv

Article Title: Mapping the spatial transcriptomic signature of the hippocampus during memory consolidation

doi: 10.1101/2023.01.18.524576

Figure Lengend Snippet: a. Design of the constructs packaged into Adeno-associated viruses (AAV) to ectopically express the dominant negative (DN) mutant of Nr4a and EGFP in the CA1 hippocampal sub-region. b. Western Blot analysis showing the time course of viral expression at 3-weeks and 4-weeks after viral infusion. One-way Anova: Šídák’s multiple comparisons test: eGFP vs Nr4ADN. n=2-3/group. c. Immunohistochemistry against YFP to detect the localization and spread of the AAV in the dorsal hippocampus. d. Experimental timeline of AAV-infusion into CA1 excitatory neurons followed by spatial learning paradigm. e. Long-term memory assessment by evaluating preference for the displaced object (DO) in a spatial object recognition (SOR) task. 2-way Anova: Significant sessions (Train-Test) x virus (Nr4ADN-eGFP) interaction: F (1, 18) = 4.537, p=0.0472, main effect of sessions: F (1, 18) = 29.93, p<0.0001 and main effect of virus: F (1, 18) = 10.26, p=0.0049. Šídák’s multiple comparisons test: eGFP: train vs test: p<0.0001, eGFP (test) vs Nr4ADN (Test): p=0.0014. n=10/group f. Total exploration time of all the objects during SOR for both the experimental groups.

Article Snippet: AAV 2.2 -CaMKIIα-Nr4ADN and AAV 2.2 -CaMKIIα-EGFP were purchased from VectorBuilder (VectorBuilder Inc).

Techniques: Construct, Dominant Negative Mutation, Mutagenesis, Western Blot, Expressing, Immunohistochemistry