renilla polya Search Results


plasmids  (Addgene inc)
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Addgene inc plasmids
Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/renilla+polya/pSF4+TetCMV+5'TOP+intron+20xGCN4+Renilla+FKBP+Stop+24xMS2v5+SV40+CTE+polyA+(Plasmid+%23119946)/pm38363833-180-31-35
Average 92 stars, based on 1 article reviews
plasmids - by Bioz Stars, 2026-09
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suntag renilla ms2 plasmid  (Addgene inc)
93
Addgene inc suntag renilla ms2 plasmid
Suntag Renilla Ms2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/renilla+polya/pSF4+TetCMV+intron+20xGCN4+Renilla+FKBP+Stop+24xMS2v5+SV40+CTE+polyA+(Plasmid+%23119945)/pmc12350179-388-16-18
Average 93 stars, based on 1 article reviews
suntag renilla ms2 plasmid - by Bioz Stars, 2026-09
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pgl3 basic luciferase reporter  (Addgene inc)
93
Addgene inc pgl3 basic luciferase reporter
IRF2 promotes OAS3 expression in unstressed cells. ( A and B ) The levels of OAS3, IRF2, RNase L, and GAPDH were analyzed in the indicated U2OS ( A ) or A549 ( B ) cell lines by western blot. ( C ) The OAS3 mRNA levels were monitored by RT-qPCR in indicated U2OS ( Left ) or A549 ( Right ) cell lines. Mean values ± SD (n = 3). **** P < 0.0001 (two-tailed t test). ( D ) The levels of OAS3 were analyzed by western blot using indicated antibodies in A549 IRF2 KO cells expressing either wild-type IRF2 or DNA binding mutant IRF2K78R. ( E and F ) Total RNAs were isolated from A549 WT or IRF2 KO cells expressing wild-type IRF2, IRF2K78R, or OAS3 and monitored for integrity by a bioanalyzer after transfection with poly(I:C) (10 ng/mL, 4 h). The red arrows indicated ribosomal RNA cleavage products. ( G ) IgG and IRF2 ChIP-sequencing in A549 cells. Analysis of ChIP-sequencing data focuses on the promoter region of OAS3. (H) IRF2 ChIP was performed in A549 WT and IRF2 KO cells. IRF2 binding on the OAS3 promoter was determined by qPCR. Mean values ± SD. *** P < 0.001 (two-tailed t test). ( I ) Schematic of <t>pGL3-OAS3-luciferase</t> constructs harboring OAS3 WT promotor region (−1 to −900 bp upstream of transcription start site [TSS]) and IRF2 binding motif region deleted (Δ−1 to −200 bp). ( J ) The relative luciferase activity (ratio of Firefly:Renilla) was measured in U2OS cells transiently transfected with the empty pGL3 and pGL3 vector harboring either WT and Δ200 bp OAS3 promoter region. ( K ) The relative luciferase activity was monitored U2OS WT or IRF2 KO cells 24 h following transfection with the pGL3-OAS3 vector.
Pgl3 Basic Luciferase Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/renilla+polya/pGL3basic+luciferase+and+renilla_polyA+(Plasmid+%23128046)/pmc11551408-160-18-22
Average 93 stars, based on 1 article reviews
pgl3 basic luciferase reporter - by Bioz Stars, 2026-09
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primer sequences 5  (Addgene inc)
91
Addgene inc primer sequences 5
IRF2 promotes OAS3 expression in unstressed cells. ( A and B ) The levels of OAS3, IRF2, RNase L, and GAPDH were analyzed in the indicated U2OS ( A ) or A549 ( B ) cell lines by western blot. ( C ) The OAS3 mRNA levels were monitored by RT-qPCR in indicated U2OS ( Left ) or A549 ( Right ) cell lines. Mean values ± SD (n = 3). **** P < 0.0001 (two-tailed t test). ( D ) The levels of OAS3 were analyzed by western blot using indicated antibodies in A549 IRF2 KO cells expressing either wild-type IRF2 or DNA binding mutant IRF2K78R. ( E and F ) Total RNAs were isolated from A549 WT or IRF2 KO cells expressing wild-type IRF2, IRF2K78R, or OAS3 and monitored for integrity by a bioanalyzer after transfection with poly(I:C) (10 ng/mL, 4 h). The red arrows indicated ribosomal RNA cleavage products. ( G ) IgG and IRF2 ChIP-sequencing in A549 cells. Analysis of ChIP-sequencing data focuses on the promoter region of OAS3. (H) IRF2 ChIP was performed in A549 WT and IRF2 KO cells. IRF2 binding on the OAS3 promoter was determined by qPCR. Mean values ± SD. *** P < 0.001 (two-tailed t test). ( I ) Schematic of <t>pGL3-OAS3-luciferase</t> constructs harboring OAS3 WT promotor region (−1 to −900 bp upstream of transcription start site [TSS]) and IRF2 binding motif region deleted (Δ−1 to −200 bp). ( J ) The relative luciferase activity (ratio of Firefly:Renilla) was measured in U2OS cells transiently transfected with the empty pGL3 and pGL3 vector harboring either WT and Δ200 bp OAS3 promoter region. ( K ) The relative luciferase activity was monitored U2OS WT or IRF2 KO cells 24 h following transfection with the pGL3-OAS3 vector.
Primer Sequences 5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/renilla+polya/pSF4+TetCMV+intron+Renilla+STOP+12xPP7+TNF%CE%B1-ARE+xrRNA12+24xMS2+SV40+CTE+polyA+(Plasmid+%23104096)/bio_rxiv__2020__07__31__231860-120-24-21
Average 91 stars, based on 1 article reviews
primer sequences 5 - by Bioz Stars, 2026-09
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plasmid psf4 tetcmv  (Addgene inc)
92
Addgene inc plasmid psf4 tetcmv
IRF2 promotes OAS3 expression in unstressed cells. ( A and B ) The levels of OAS3, IRF2, RNase L, and GAPDH were analyzed in the indicated U2OS ( A ) or A549 ( B ) cell lines by western blot. ( C ) The OAS3 mRNA levels were monitored by RT-qPCR in indicated U2OS ( Left ) or A549 ( Right ) cell lines. Mean values ± SD (n = 3). **** P < 0.0001 (two-tailed t test). ( D ) The levels of OAS3 were analyzed by western blot using indicated antibodies in A549 IRF2 KO cells expressing either wild-type IRF2 or DNA binding mutant IRF2K78R. ( E and F ) Total RNAs were isolated from A549 WT or IRF2 KO cells expressing wild-type IRF2, IRF2K78R, or OAS3 and monitored for integrity by a bioanalyzer after transfection with poly(I:C) (10 ng/mL, 4 h). The red arrows indicated ribosomal RNA cleavage products. ( G ) IgG and IRF2 ChIP-sequencing in A549 cells. Analysis of ChIP-sequencing data focuses on the promoter region of OAS3. (H) IRF2 ChIP was performed in A549 WT and IRF2 KO cells. IRF2 binding on the OAS3 promoter was determined by qPCR. Mean values ± SD. *** P < 0.001 (two-tailed t test). ( I ) Schematic of <t>pGL3-OAS3-luciferase</t> constructs harboring OAS3 WT promotor region (−1 to −900 bp upstream of transcription start site [TSS]) and IRF2 binding motif region deleted (Δ−1 to −200 bp). ( J ) The relative luciferase activity (ratio of Firefly:Renilla) was measured in U2OS cells transiently transfected with the empty pGL3 and pGL3 vector harboring either WT and Δ200 bp OAS3 promoter region. ( K ) The relative luciferase activity was monitored U2OS WT or IRF2 KO cells 24 h following transfection with the pGL3-OAS3 vector.
Plasmid Psf4 Tetcmv, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/renilla+polya/pSF4+TetCMV+intron+20xGCN4+Renilla+Rh1+STOP+24xMS2v5+SV40+CTE+polyA+(Plasmid+%23105000)/10__7554_slash_elife__85135-469-18-31
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psf4 cmv intron renilla trick cte polya (plasmid #84443)  (Addgene inc)
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psf4 tetcmv intron renilla stop 12xpp7 1xfirefly-sirna xrrna12 24xms2 sv40 cte polya (plasmid #104094)  (Addgene inc)
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psf4 tetcmv 5'top intron renilla-stop 24xpp7 1xfirefly-sirna xrrna12 24xms2 sv40 cte polya (plasmid #104140)  (Addgene inc)
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psf4 tetcmv intron 4xgcn4 renilla fkbp stop 24xms2v5 sv40 cte polya (plasmid #104984)  (Addgene inc)
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psf4 tetcmv intron renilla-stop 24xpp7 1xfirefly-sirna xrrna12 24xms2 sv40 cte polya (plasmid #104095)  (Addgene inc)
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psf4 cmv 5top intron renilla trick cte polya (plasmid #64544)  (Addgene inc)
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psf4 tetcmv intron renilla-stop 24xpp7 1xfirefly-sirna 24xms2 sv40 cte polya (plasmid #104097)  (Addgene inc)
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Image Search Results


IRF2 promotes OAS3 expression in unstressed cells. ( A and B ) The levels of OAS3, IRF2, RNase L, and GAPDH were analyzed in the indicated U2OS ( A ) or A549 ( B ) cell lines by western blot. ( C ) The OAS3 mRNA levels were monitored by RT-qPCR in indicated U2OS ( Left ) or A549 ( Right ) cell lines. Mean values ± SD (n = 3). **** P < 0.0001 (two-tailed t test). ( D ) The levels of OAS3 were analyzed by western blot using indicated antibodies in A549 IRF2 KO cells expressing either wild-type IRF2 or DNA binding mutant IRF2K78R. ( E and F ) Total RNAs were isolated from A549 WT or IRF2 KO cells expressing wild-type IRF2, IRF2K78R, or OAS3 and monitored for integrity by a bioanalyzer after transfection with poly(I:C) (10 ng/mL, 4 h). The red arrows indicated ribosomal RNA cleavage products. ( G ) IgG and IRF2 ChIP-sequencing in A549 cells. Analysis of ChIP-sequencing data focuses on the promoter region of OAS3. (H) IRF2 ChIP was performed in A549 WT and IRF2 KO cells. IRF2 binding on the OAS3 promoter was determined by qPCR. Mean values ± SD. *** P < 0.001 (two-tailed t test). ( I ) Schematic of pGL3-OAS3-luciferase constructs harboring OAS3 WT promotor region (−1 to −900 bp upstream of transcription start site [TSS]) and IRF2 binding motif region deleted (Δ−1 to −200 bp). ( J ) The relative luciferase activity (ratio of Firefly:Renilla) was measured in U2OS cells transiently transfected with the empty pGL3 and pGL3 vector harboring either WT and Δ200 bp OAS3 promoter region. ( K ) The relative luciferase activity was monitored U2OS WT or IRF2 KO cells 24 h following transfection with the pGL3-OAS3 vector.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: A CRISPR-Cas9 knockout screening identifies IRF2 as a key driver of OAS3/RNase L-mediated RNA decay during viral infection

doi: 10.1073/pnas.2412725121

Figure Lengend Snippet: IRF2 promotes OAS3 expression in unstressed cells. ( A and B ) The levels of OAS3, IRF2, RNase L, and GAPDH were analyzed in the indicated U2OS ( A ) or A549 ( B ) cell lines by western blot. ( C ) The OAS3 mRNA levels were monitored by RT-qPCR in indicated U2OS ( Left ) or A549 ( Right ) cell lines. Mean values ± SD (n = 3). **** P < 0.0001 (two-tailed t test). ( D ) The levels of OAS3 were analyzed by western blot using indicated antibodies in A549 IRF2 KO cells expressing either wild-type IRF2 or DNA binding mutant IRF2K78R. ( E and F ) Total RNAs were isolated from A549 WT or IRF2 KO cells expressing wild-type IRF2, IRF2K78R, or OAS3 and monitored for integrity by a bioanalyzer after transfection with poly(I:C) (10 ng/mL, 4 h). The red arrows indicated ribosomal RNA cleavage products. ( G ) IgG and IRF2 ChIP-sequencing in A549 cells. Analysis of ChIP-sequencing data focuses on the promoter region of OAS3. (H) IRF2 ChIP was performed in A549 WT and IRF2 KO cells. IRF2 binding on the OAS3 promoter was determined by qPCR. Mean values ± SD. *** P < 0.001 (two-tailed t test). ( I ) Schematic of pGL3-OAS3-luciferase constructs harboring OAS3 WT promotor region (−1 to −900 bp upstream of transcription start site [TSS]) and IRF2 binding motif region deleted (Δ−1 to −200 bp). ( J ) The relative luciferase activity (ratio of Firefly:Renilla) was measured in U2OS cells transiently transfected with the empty pGL3 and pGL3 vector harboring either WT and Δ200 bp OAS3 promoter region. ( K ) The relative luciferase activity was monitored U2OS WT or IRF2 KO cells 24 h following transfection with the pGL3-OAS3 vector.

Article Snippet: The OAS3 luciferase reporter plasmid was generated by cloning the OAS3 promoter region (TSS −1 to −900) into pGL3 basic luciferase reporter (Addgene #128046).

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Two Tailed Test, Binding Assay, Mutagenesis, Isolation, Transfection, ChIP-sequencing, Luciferase, Construct, Activity Assay, Plasmid Preparation