recombinant sema3a Search Results


sema3a protein, mouse, recombinant  (TargetMol)
N/A
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sema3a recombinant mouse protein  (Thermo Fisher)
N/A
Sema Domain, Immunoglobulin Domain (Ig) Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins
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recombinant ap-sema3a protein  (ScreenFect GmbH)
90
ScreenFect GmbH recombinant ap-sema3a protein
Binding characteristics of <t>Sema3A</t> to cell surface receptors in HaCaT cells. A: Displacement profile of kCer on AP-Sema3A binding to cell surface receptors. Cells were cultured in a 24-well microplate and treated with 15.3 APU of 22.3 nM AP-Sema3A in the presence of Sema3A (10–100 nM) (upper graph) or kCer (10–100 μM) (lower graph). Cells were washed and incubated at 65 °C for 30 min, and the remaining AP activity was measured using BCIP/NBT Phosphatase Substrate and Imaging-J software. Data are shown as the means ± SD (n = 4). B: AP-Sema3A binding to cells in the presence of Sema3A (0, 10, 50, and 100 nM) or kCer (0, 10, 50, 100 μM). C: Displacement profile of AP-Sema3A binding by Sema3A (50 nM), kCer, kGlcCer, C16Cer, C18Cer, and C24Cer (50 μM, respectively).
Recombinant Ap Sema3a Protein, supplied by ScreenFect GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+sema3a/recombinant+ap+sema3a+protein/pmc06319021-54-0-20
Average 90 stars, based on 1 article reviews
recombinant ap-sema3a protein - by Bioz Stars, 2026-09
90/100 stars
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sema3a protein concentration  (Elabscience Biotechnology)
94
Elabscience Biotechnology sema3a protein concentration
Binding characteristics of <t>Sema3A</t> to cell surface receptors in HaCaT cells. A: Displacement profile of kCer on AP-Sema3A binding to cell surface receptors. Cells were cultured in a 24-well microplate and treated with 15.3 APU of 22.3 nM AP-Sema3A in the presence of Sema3A (10–100 nM) (upper graph) or kCer (10–100 μM) (lower graph). Cells were washed and incubated at 65 °C for 30 min, and the remaining AP activity was measured using BCIP/NBT Phosphatase Substrate and Imaging-J software. Data are shown as the means ± SD (n = 4). B: AP-Sema3A binding to cells in the presence of Sema3A (0, 10, 50, and 100 nM) or kCer (0, 10, 50, 100 μM). C: Displacement profile of AP-Sema3A binding by Sema3A (50 nM), kCer, kGlcCer, C16Cer, C18Cer, and C24Cer (50 μM, respectively).
Sema3a Protein Concentration, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+sema3a/Recombinant+Human+SEMA3A+Protein/10__1016_slash_j__jdermsci__2025__12__001-83-0-11
Average 94 stars, based on 1 article reviews
sema3a protein concentration - by Bioz Stars, 2026-09
94/100 stars
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recombinant human semaphorin 3a fc chimera protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant human semaphorin 3a fc chimera protein, cf
Binding characteristics of <t>Sema3A</t> to cell surface receptors in HaCaT cells. A: Displacement profile of kCer on AP-Sema3A binding to cell surface receptors. Cells were cultured in a 24-well microplate and treated with 15.3 APU of 22.3 nM AP-Sema3A in the presence of Sema3A (10–100 nM) (upper graph) or kCer (10–100 μM) (lower graph). Cells were washed and incubated at 65 °C for 30 min, and the remaining AP activity was measured using BCIP/NBT Phosphatase Substrate and Imaging-J software. Data are shown as the means ± SD (n = 4). B: AP-Sema3A binding to cells in the presence of Sema3A (0, 10, 50, and 100 nM) or kCer (0, 10, 50, 100 μM). C: Displacement profile of AP-Sema3A binding by Sema3A (50 nM), kCer, kGlcCer, C16Cer, C18Cer, and C24Cer (50 μM, respectively).
Recombinant Human Semaphorin 3a Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+sema3a/Recombinant+Human+Semaphorin+3A+Fc+Chimera+Protein%2C+CF/bio-techne+corporation___1250-s3
Average 94 stars, based on 1 article reviews
recombinant human semaphorin 3a fc chimera protein, cf - by Bioz Stars, 2026-09
94/100 stars
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recombinant mouse semaphorin 3a fc chimera protein, cf  (Bio-Techne corporation)
94
Bio-Techne corporation recombinant mouse semaphorin 3a fc chimera protein, cf
Binding characteristics of <t>Sema3A</t> to cell surface receptors in HaCaT cells. A: Displacement profile of kCer on AP-Sema3A binding to cell surface receptors. Cells were cultured in a 24-well microplate and treated with 15.3 APU of 22.3 nM AP-Sema3A in the presence of Sema3A (10–100 nM) (upper graph) or kCer (10–100 μM) (lower graph). Cells were washed and incubated at 65 °C for 30 min, and the remaining AP activity was measured using BCIP/NBT Phosphatase Substrate and Imaging-J software. Data are shown as the means ± SD (n = 4). B: AP-Sema3A binding to cells in the presence of Sema3A (0, 10, 50, and 100 nM) or kCer (0, 10, 50, 100 μM). C: Displacement profile of AP-Sema3A binding by Sema3A (50 nM), kCer, kGlcCer, C16Cer, C18Cer, and C24Cer (50 μM, respectively).
Recombinant Mouse Semaphorin 3a Fc Chimera Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+sema3a/Recombinant+Mouse+Semaphorin+3A+Fc+Chimera+Protein%2C+CF/bio-techne+corporation___5926-s3-cf
Average 94 stars, based on 1 article reviews
recombinant mouse semaphorin 3a fc chimera protein, cf - by Bioz Stars, 2026-09
94/100 stars
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recombinant rat sema3a protein  (Creative BioMart)
N/A
Recombinant Rat SEMA3A full length or partial length protein was expressed.http://www.creativebiomart.net/Recombinant-Rat-SEMA3A-Protein-454136.htm
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recombinant human sema3a protein, his-tagged  (Creative BioMart)
N/A
Recombinant Human SEMA3A(Asn21-Val771) fused with His tag at C-terminal was expressed in HEK293.http://www.creativebiomart.net/Recombinant-Human-SEMA3A-protein-His-tagged-456622.htm
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sema3a protein, human, recombinant  (TargetMol)
N/A
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recombinant mouse semaphorin 3a/sema3a protein  (Elabscience Biotechnology)
N/A
Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 500 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous
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recombinant mouse sema3a protein, fc chimera  (Creative BioMart)
N/A
Recombinant Mouse Sema3a (Accession # O08665), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.http://www.creativebiomart.net/description_437578_12.htm
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recombinant human semaphorin-3a/sema3a  (Beijing Solarbio Science)
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Image Search Results


Binding characteristics of Sema3A to cell surface receptors in HaCaT cells. A: Displacement profile of kCer on AP-Sema3A binding to cell surface receptors. Cells were cultured in a 24-well microplate and treated with 15.3 APU of 22.3 nM AP-Sema3A in the presence of Sema3A (10–100 nM) (upper graph) or kCer (10–100 μM) (lower graph). Cells were washed and incubated at 65 °C for 30 min, and the remaining AP activity was measured using BCIP/NBT Phosphatase Substrate and Imaging-J software. Data are shown as the means ± SD (n = 4). B: AP-Sema3A binding to cells in the presence of Sema3A (0, 10, 50, and 100 nM) or kCer (0, 10, 50, 100 μM). C: Displacement profile of AP-Sema3A binding by Sema3A (50 nM), kCer, kGlcCer, C16Cer, C18Cer, and C24Cer (50 μM, respectively).

Journal: Biochemistry and Biophysics Reports

Article Title: Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism

doi: 10.1016/j.bbrep.2018.11.004

Figure Lengend Snippet: Binding characteristics of Sema3A to cell surface receptors in HaCaT cells. A: Displacement profile of kCer on AP-Sema3A binding to cell surface receptors. Cells were cultured in a 24-well microplate and treated with 15.3 APU of 22.3 nM AP-Sema3A in the presence of Sema3A (10–100 nM) (upper graph) or kCer (10–100 μM) (lower graph). Cells were washed and incubated at 65 °C for 30 min, and the remaining AP activity was measured using BCIP/NBT Phosphatase Substrate and Imaging-J software. Data are shown as the means ± SD (n = 4). B: AP-Sema3A binding to cells in the presence of Sema3A (0, 10, 50, and 100 nM) or kCer (0, 10, 50, 100 μM). C: Displacement profile of AP-Sema3A binding by Sema3A (50 nM), kCer, kGlcCer, C16Cer, C18Cer, and C24Cer (50 μM, respectively).

Article Snippet: Recombinant AP-Sema3A protein was expressed in HEK293 cells that were transiently transfected with an expression plasmid using a Lipofection Kit (ScreenFecT™A plus, Wako Co.).

Techniques: Binding Assay, Cell Culture, Incubation, Activity Assay, Imaging, Software

Cell migration profile of HaCaT cells. Media containing complete sera induced migration of HaCaT cells in a 24-well chamber plate assay. Cell migration activity was determined quantitatively using Image-J after GIEMSA'S AZUR EOSIN Methylene Blue-staining of transwell filter membrane. A: The following were added to the bottom wells of a 24-well Boyden chamber: No addition (None); Sema3A (10 nM); kCer (25 μM); His (1 μM); CCL16 (10 nM, His agonist for H4R); and CCL17 (10 nM, non-His agonist). B: Cell migration was quantitated as % of control (no addition). Data are shown as the means ± SD (n = 4). *P < 0.05, **P < 0.001 vs. vehicle-treated control.

Journal: Biochemistry and Biophysics Reports

Article Title: Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism

doi: 10.1016/j.bbrep.2018.11.004

Figure Lengend Snippet: Cell migration profile of HaCaT cells. Media containing complete sera induced migration of HaCaT cells in a 24-well chamber plate assay. Cell migration activity was determined quantitatively using Image-J after GIEMSA'S AZUR EOSIN Methylene Blue-staining of transwell filter membrane. A: The following were added to the bottom wells of a 24-well Boyden chamber: No addition (None); Sema3A (10 nM); kCer (25 μM); His (1 μM); CCL16 (10 nM, His agonist for H4R); and CCL17 (10 nM, non-His agonist). B: Cell migration was quantitated as % of control (no addition). Data are shown as the means ± SD (n = 4). *P < 0.05, **P < 0.001 vs. vehicle-treated control.

Article Snippet: Recombinant AP-Sema3A protein was expressed in HEK293 cells that were transiently transfected with an expression plasmid using a Lipofection Kit (ScreenFecT™A plus, Wako Co.).

Techniques: Migration, Activity Assay, Staining

Combined effect of Sema3A or kCer with His or CCL chemokines (CCL16 or CCL17) on cell migration. A: Migration of HaCaT cells stimulated by no addition (None), Sema3A (100 nM), or kCer (25 μM) plus His (1 μM), CCL16 (10 nM), or CCL17 (10 nM). B: Effect of 100 nM Sema3A on cell migration by no other addition (None), 1 μM His, 10 nM CCL16 or 10 nM CCL17. C: Effect of 100 μM kCer on cell migration by no other addition (None), 1 μM His, 10 nM CCL16 or 10 nM CCL17. Cell migration was quantitated as % of control (no addition). Data are shown as the means ± SD (n = 4). *P < 0.05.

Journal: Biochemistry and Biophysics Reports

Article Title: Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism

doi: 10.1016/j.bbrep.2018.11.004

Figure Lengend Snippet: Combined effect of Sema3A or kCer with His or CCL chemokines (CCL16 or CCL17) on cell migration. A: Migration of HaCaT cells stimulated by no addition (None), Sema3A (100 nM), or kCer (25 μM) plus His (1 μM), CCL16 (10 nM), or CCL17 (10 nM). B: Effect of 100 nM Sema3A on cell migration by no other addition (None), 1 μM His, 10 nM CCL16 or 10 nM CCL17. C: Effect of 100 μM kCer on cell migration by no other addition (None), 1 μM His, 10 nM CCL16 or 10 nM CCL17. Cell migration was quantitated as % of control (no addition). Data are shown as the means ± SD (n = 4). *P < 0.05.

Article Snippet: Recombinant AP-Sema3A protein was expressed in HEK293 cells that were transiently transfected with an expression plasmid using a Lipofection Kit (ScreenFecT™A plus, Wako Co.).

Techniques: Migration

Proposed mechanism for kCer acting as a Sema3A-like ligand. Sema3A binding: Sema3A binds to Nrp1, recruiting a monomer from an inactive PlexA dimer, resulting in the formation of a hetero-pentameric complex of Sema3A, Nrp1 dimer, and PlexA. Signaling pathway downstream molecule of the Sema3A, Cofilin is phosphorylated, resulting in suppression of actin turnover, causing an inhibition of cell migration. kCer also binds as a Sema3A-like ligand to Nrp1 without Sema3A and forms of the receptor complex to induces an inhibitory effect on cell migration.

Journal: Biochemistry and Biophysics Reports

Article Title: Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism

doi: 10.1016/j.bbrep.2018.11.004

Figure Lengend Snippet: Proposed mechanism for kCer acting as a Sema3A-like ligand. Sema3A binding: Sema3A binds to Nrp1, recruiting a monomer from an inactive PlexA dimer, resulting in the formation of a hetero-pentameric complex of Sema3A, Nrp1 dimer, and PlexA. Signaling pathway downstream molecule of the Sema3A, Cofilin is phosphorylated, resulting in suppression of actin turnover, causing an inhibition of cell migration. kCer also binds as a Sema3A-like ligand to Nrp1 without Sema3A and forms of the receptor complex to induces an inhibitory effect on cell migration.

Article Snippet: Recombinant AP-Sema3A protein was expressed in HEK293 cells that were transiently transfected with an expression plasmid using a Lipofection Kit (ScreenFecT™A plus, Wako Co.).

Techniques: Binding Assay, Inhibition, Migration