Journal: ACS ES&T water

Article Title: Simple Affinity-Based Method for Concentrating Viruses from Wastewater Using Engineered Curli Fibers

doi: 10.1021/acsestwater.1c00208

Figure Lengend Snippet: Spin down of purified, recombinant SARS-CoV-2 spike protein from PBS using engineered curli fibers. A live-cell ELISA assay was developed to probe for the presence of spike protein in the curli-cell pellet following the spin-down protocol. The four bars on the left correspond to various negative controls, and the remaining 10 bars correspond to samples where the curli fibers were engineered to display protein or peptide domains with putative binding affinity for the SARS-CoV-2 spike protein. Higher absorbance readings correspond to increased immobilization of the antibody used to detect the presence of the recombinant spike protein in the cell pellet. Values and error bars reflect the mean ± s.e. of three independent experiments ( n = 3).

Article Snippet: The mixture was incubated end-over-end at room temperature for 5 min. Then, 1 μ L of recombinant SARS-CoV-2 spike protein subunit S1 with hFc and His tags (Abclonal RP01259) was added to reach a final concentration of 0.2 μ g/mL.

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay