Journal: Haematologica

Article Title: Hepatocyte growth factor promotes migration of human myeloma cells.

doi: 10.3324/haematol.11867

Figure Lengend Snippet: Figure 1. INA-6 cells (A) or primary MM cells (B) were seeded in the upper compartment of a two-chamber Transwell migration assay. To the bottom compartment was added RPMI-medium with or without FGF2 (10 ng/mL), HGF (150 ng/mL), IGF-1 (100 ng/mL), IL-15 (20 ng/mL), SDF-1α (75 ng/mL), TNF (20 ng/mL) or VEGF (100 ng/mL). After 22 hrs. at 37°C, cells in the bottom compartments were counted and percentage of migrated cells calculated. Error bars represent +1 standard deviation (SD) of three repeated counts in two independent measurements. A is representative for three similar experiments. B represents 1 out of 5 primary cell samples. Migration increased significantly when cells were subjected to specific cytokines, according to an unpaired Student’s two-tailed test, *(p<0.01).

Article Snippet: Recombinant human FGF2, IGF-1, interleukin (IL)-15 and VEGF were from R&D Systems Inc. (Minneapolis, MN, USA), IL-6 was from Biosource (Camarillo, CA, USA) and SDF-1α from Peprotech (London, UK).

Techniques: Transwell Migration Assay, Standard Deviation, Migration, Two Tailed Test

Journal: Molecular Oncology

Article Title: Fibroblast growth factor signals regulate transforming growth factor‐β‐induced endothelial‐to‐myofibroblast transition of tumor endothelial cells via Elk1

doi: 10.1002/1878-0261.12504

Figure Lengend Snippet: Regulation of FGF signals by TGF ‐β2 in TEC s. (A, B) Effect of TGF ‐β2 on the expression of FGF 2 in TEC s. TEC s were cultured in the absence (−) or presence (+) of TGF ‐β2 for 72 h, followed by qRT ‐ PCR (A) and immunoblot (B) analyses for the expression of FGF 2. rFGF2, recombinant FGF2. (C) Effect of TGF ‐β2 on the ERK 1/2 phosphorylation in TEC s. TEC s were cultured in the absence (−) or presence (+) of TGF ‐β2 for 72 h, followed by immunoblot analysis using phospho‐(P)‐ ERK 1/2, and total ERK 1/2 antibodies. (D, E) Effect of endogenous FGF 2 on α‐ SMA expression and the ERK 1/2 phosphorylation in TEC s. TEC s were cultured with TGF ‐β2 in combination with control‐IgG or anti‐ FGF 2 antibodies for 72 h, followed by qRT ‐ PCR analysis for α‐ SMA expression (D) and immunoblot analysis using phospho‐(P)‐ ERK 1/2, and total ERK 1/2 antibodies (E). Error bars represent standard deviation. Student's t ‐test with two biological independent replicates was used to determine statistical significance; * P < 0.05.

Article Snippet: Human TGF‐β2 (302‐B2‐010; R&D systems, Minneapolis, MN, USA: 1 ng·mL −1 ), human FGF2 (233‐FB‐010; R&D systems: 50 ng·mL −1 or Peprotech; Rocky Hill, NJ, USA), Infigratinib (I‐1500; LC Laboratories, Woburn, MA, USA: 3 μ m ), VEGF‐A (30 ng·mL −1 ; R&D), and SB431542 (10 μ m ; Wako, Saitama, Japan) were used in each experiment.

Techniques: Expressing, Cell Culture, Quantitative RT-PCR, Western Blot, Recombinant, Phospho-proteomics, Control, Standard Deviation