Journal:

Article Title: Selective Inhibition of the C5a Chemotactic Cofactor Function of the Vitamin D Binding Protein by 1,25(OH) 2 Vitamin D 3

doi: 10.1016/j.molimm.2005.07.023

Figure Lengend Snippet: Effect of 1,25(OH)2D3 on chemotaxis to C5a, LTB4, formyl peptide and CXCL8. Purified DBP (100 nM) was treated for 5 min at 37°C with 10 nM 1,25(OH)2D3. Neutrophils (4 x 106/ml) were then preincubated for 40 min at 37°C with either DBP plus 1,25(OH)2D3 or buffer. Neutrophil movement then was assayed for 30 min at 37°C. Numbers represent mean ± SEM of 3 experiments using cells from different donors.

Article Snippet: Recombinant CXCL8 (IL-8) was obtained from R&D Systems (Minneapolis, MN).

Techniques: Chemotaxis Assay, Purification

Journal: Scientific reports

Article Title: Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells.

doi: 10.1038/s41598-017-15081-6

Figure Lengend Snippet: Figure 2. Chemokine screening strategy for hPSCs. (a–d) Immunofluorescence of pluripotency markers TRA- 1-81 and Oct4 on hPSCs. KSR medium (a,b); mTeSR1 medium (c,d); hESCs (a,c); hiPSCs (d); Feeder cells (b); Scale bars, 50 μm (a–d; arrows) and 10 μm (insets). Cells were counterstained with DAPI. (e) Chemokine screening strategy for hPSCs cultured in KSR (red) or mTeSR1 (green) media.

Article Snippet: Recombinant human chemokine proteins IL-8, SDF-1α, and IP-10 were purchased from R&D Systems (USA).

Techniques: Immunofluorescence, Cell Culture

Journal: Scientific reports

Article Title: Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells.

doi: 10.1038/s41598-017-15081-6

Figure Lengend Snippet: Figure 4. Chemokine signaling functionally mediates the transmigration of hPSCs. Chemoattractant effect of exogenous IP-10, IL-8, and SDF-1α on hPSCs. hESCs (a,b,e,f,i,j); hiPSCs (c,d,g,h,k,l); IL-8 (a,c) and CXCR2 antagonist SB265610 (b,d); SDF-1α (e,g) and CXCR4 antagonist AMD3100 (f,h); IP-10 (i,k) and CXCR3 antagonist NBI74330 (j,l). Values on graphs represent means ± sem, n = 3 individual experiments. *P < 0.05, **P < 0.01.

Article Snippet: Recombinant human chemokine proteins IL-8, SDF-1α, and IP-10 were purchased from R&D Systems (USA).

Techniques: Transmigration Assay

Journal: Scientific reports

Article Title: Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells.

doi: 10.1038/s41598-017-15081-6

Figure Lengend Snippet: Figure 5. Chemokine IL-8 facilitates the differentiation of hPSCs. Stemness of hPSCs was evaluated by the expressions of three pluripotency genes (Oct4, Nanog, and Rex-1) after treatment with IL-8 and CXCR2 antagonists reparixin/SB265610. hESCs (a–c); hiPSCs (d–f); IL-8 (a,d); Reparixin (b,e); and SB265610 (c,f). Values on graphs represent means ± sem, n = 3 individual experiments. *P < 0.05, **P < 0.01, ***P < 0. 001.

Article Snippet: Recombinant human chemokine proteins IL-8, SDF-1α, and IP-10 were purchased from R&D Systems (USA).

Techniques:

Journal: Scientific reports

Article Title: Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells.

doi: 10.1038/s41598-017-15081-6

Figure Lengend Snippet: Figure 7. Mobility and maintenance of hPSCs require multiple chemokine signals. Schematic representation of predominant chemokines from feeder cells or hPSCs themselves. 1) Released chemokines uniformly mediate the migration of hPSCs; 2) Both differentiating and maintaining chemokines were secreted in the culture supernatant. However, the final effect of chemokines tended to maintain the stemness of hPSCs in vitro.

Article Snippet: Recombinant human chemokine proteins IL-8, SDF-1α, and IP-10 were purchased from R&D Systems (USA).

Techniques: Migration, In Vitro