Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-21 receptor in epidermis from patients with systemic sclerosis.

doi: 10.1002/art.20883

Figure Lengend Snippet: Figure 1. Expression of interleukin-21 receptor (IL-21R) in keratinocytes from patients with systemic sclerosis (SSc). In situ hybridization showed abundant expression of IL-21R in skin biopsy specimens from all SSc patients but in none of the specimens from healthy controls. a, In situ hybridization with antisense probes specific for IL-21R. b, Control experiment using sense probes. c, In situ hybridization with antisense probes specific for IL-21R in paraffin-embedded skin sections from another SSc patient. d, Incubation with sense probes in the same sample as in c. Representative results shown are from 1 of 2 independent experiments. Note the unstained melanocytes at the epidermal–dermal border (arrows). (Original magnification 200.) Color figure can be viewed in the online issue, which is available at http://www.arthritisrheum.org.

Article Snippet: Membranes were blocked in 5% nonfat dry milk with 0.05% Tween 20 in Tris buffered saline (TBS) (pH 7.4) for 1 hour at room temperature before probing overnight at 4°C with antibodies against IL-21R (0.5 g/ml) (ProSci).

Techniques: Expressing, In Situ Hybridization, Control, Incubation

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-21 receptor in epidermis from patients with systemic sclerosis.

doi: 10.1002/art.20883

Figure Lengend Snippet: Figure 2. Expression of interleukin-21 receptor (IL-21R) in skin samples obtained from healthy controls. No signal was detected in any of the normal skin specimens by in situ hybridization. a, In situ hybridization with antisense probes for IL-21R. b, Control experiment with sense probes. (Original magnification 200.) Color figure can be viewed in the online issue, which is available at http:// www.arthritisrheum.org.

Article Snippet: Membranes were blocked in 5% nonfat dry milk with 0.05% Tween 20 in Tris buffered saline (TBS) (pH 7.4) for 1 hour at room temperature before probing overnight at 4°C with antibodies against IL-21R (0.5 g/ml) (ProSci).

Techniques: Expressing, In Situ Hybridization, Control

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-21 receptor in epidermis from patients with systemic sclerosis.

doi: 10.1002/art.20883

Figure Lengend Snippet: Figure 3. Western blot showing the expression of interleukin-21 receptor (IL-21R) protein in cultured foreskin keratinocytes. A weak signal for IL-21R was also detected in Ramos cells and Raji cell lysates. The different lanes represent protein lysates from individual patients (foreskin keratinocytes; arrow) or cell cultures (Ramos cells).

Article Snippet: Membranes were blocked in 5% nonfat dry milk with 0.05% Tween 20 in Tris buffered saline (TBS) (pH 7.4) for 1 hour at room temperature before probing overnight at 4°C with antibodies against IL-21R (0.5 g/ml) (ProSci).

Techniques: Western Blot, Expressing, Cell Culture

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-21 receptor in epidermis from patients with systemic sclerosis.

doi: 10.1002/art.20883

Figure Lengend Snippet: Figure 4. Expression of interleukin-21 receptor (IL-21R) in the SCID mouse transplantation model. Expression of IL-21R in human keratinocytes from patients with systemic sclerosis (SSc) remained unchanged after transplantation onto SCID mice for 60 days. a, In situ hybridization with antisense probes for IL-21R. Intense staining specific for IL-21R was seen in human keratinocytes of the transplanted skin biopsy specimen. No expression was observed in the dermis of the skin biopsy specimens from SSc patients or in SCID mouse tissues. Large arrows indicate human epidermis; small arrows indicate human dermis. b, No signal was detected in control experiments using sense probes for IL-21R. c, Higher magnification of a, showing abundant expression of IL-21R in the majority of keratinocytes of the human transplant. d, No signal was detected in control experiments with sense probes. (Original magnification 100 in a and b; 400 in c; 200 in d.) Color figure can be viewed in the online issue, which is available at http://www.arthritisrheum.org.

Article Snippet: Membranes were blocked in 5% nonfat dry milk with 0.05% Tween 20 in Tris buffered saline (TBS) (pH 7.4) for 1 hour at room temperature before probing overnight at 4°C with antibodies against IL-21R (0.5 g/ml) (ProSci).

Techniques: Expressing, Transplantation Assay, In Situ Hybridization, Staining, Control

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-21 receptor in epidermis from patients with systemic sclerosis.

doi: 10.1002/art.20883

Figure Lengend Snippet: Figure 5. Coexpression of interleukin-21 receptor (IL-21R) and vascular endothelial growth factor (VEGF) in serial sections of skin biopsy specimens obtained from patients with systemic sclerosis. a and c, In situ hybridization for IL-21R showing expression of IL-21 mRNA preferentially in keratinocytes but also in dermal cells. b and d, In situ hybridization for VEGF in a serial section from the same patient. (Original magnification 200 in a and b; 400 in c and d.) Color figure can be viewed in the online issue, which is available at http://www.arthritisrheum.org.

Article Snippet: Membranes were blocked in 5% nonfat dry milk with 0.05% Tween 20 in Tris buffered saline (TBS) (pH 7.4) for 1 hour at room temperature before probing overnight at 4°C with antibodies against IL-21R (0.5 g/ml) (ProSci).

Techniques: In Situ Hybridization, Expressing