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Image Search Results
Journal: The Journal of biological chemistry
Article Title: ATXN3 functions as a tumor suppresser through potentiating Galectin-9-mediated apoptosis in human colon adenocarcinoma.
doi: 10.1016/j.jbc.2024.107415
Figure Lengend Snippet: Figure 5. Targeted ATXN3 deletion inhibits colon cancer growth in mice. A–C, WT or ATXN3-KO HCT116 cells were injected subcutaneously into RAG1 mutant mice (n = 10). Tumor growth curve (A), photograph (B), and weight (C) are shown. D and E, the protein expression of Galectin-9, Ki67, Cleaved Caspase-3 in (B) tumor was detected by IHC and measured relative expression by image J software(n = 5). Scale bar: 200 mm. F–H, HCT116 cells were injected subcutaneously into RAG1 mutant mice and treated with Galectin-9 recombinant protein (n = 10). Tumor growth curve (F), photograph (G), and weight (H) are shown. I and J, the protein expression of Galectin-9, Ki67, Cleaved Caspase-3 in (G) tumor was detected by IHC and measured relative expression by image J software(n = 4). Scale bar: 200 mm. K–M, tumor growth curve (K) of RAG1 mutant mice injected subcutaneously with WT or ATXN3-KO HCT116 cells and stabilize overexpression of Galectin-9 by lentivirus (n = 20). Tumor photograph (L) and tumor weight (M) are shown. A, C, F, H, E, and J, 2- tailed unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001. L and M: ordinary 1-way ANOVA. *p < 0.05, **p < 0.01,***p < 0.001.
Article Snippet: In order to achieve in vivo overexpression of the recombinant protein Galectin-9, mice were administered intraperitoneal injections of Galectin-9
Techniques: Injection, Mutagenesis, Expressing, Software, Recombinant, Over Expression