recombinant expression vectors Search Results


cag promoter  (Genecopoeia)
94
Genecopoeia cag promoter
Cag Promoter, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-Lv235+expression+vector+for+LxR+recombination+cloning/pm41781491-84-25-27
Average 94 stars, based on 1 article reviews
cag promoter - by Bioz Stars, 2026-09
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pez m06 eukaryotic expression vector  (Genecopoeia)
94
Genecopoeia pez m06 eukaryotic expression vector
Pez M06 Eukaryotic Expression Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M06+expression+vector+for+LxR+recombination+cloning/pmc03750158-122-14-19
Average 94 stars, based on 1 article reviews
pez m06 eukaryotic expression vector - by Bioz Stars, 2026-09
94/100 stars
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pez lv201 vector  (Genecopoeia)
94
Genecopoeia pez lv201 vector
Pez Lv201 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-Lv201+expression+vector+for+L+x+R+recombination+cloning/pmc08666584-46-12-14
Average 94 stars, based on 1 article reviews
pez lv201 vector - by Bioz Stars, 2026-09
94/100 stars
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expression vector pez m09  (Genecopoeia)
94
Genecopoeia expression vector pez m09
Expression Vector Pez M09, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M09+expression+vector+for+LxR+recombination+cloning/pmc06238639-158-4-10
Average 94 stars, based on 1 article reviews
expression vector pez m09 - by Bioz Stars, 2026-09
94/100 stars
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pez m02 expression vector  (Genecopoeia)
94
Genecopoeia pez m02 expression vector
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Pez M02 Expression Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M02+expression+vector+for+LxR+recombination+cloning/pmc04106151-147-14-31
Average 94 stars, based on 1 article reviews
pez m02 expression vector - by Bioz Stars, 2026-09
94/100 stars
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expression vector pez m67  (Genecopoeia)
94
Genecopoeia expression vector pez m67
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Expression Vector Pez M67, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M67+expression+vector+for+LxR+recombination+cloning/10__1039_slash_C9MO00175A-31-5-11
Average 94 stars, based on 1 article reviews
expression vector pez m67 - by Bioz Stars, 2026-09
94/100 stars
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ezshuttle recombination cloning expression vector pez lv199  (Genecopoeia)
94
Genecopoeia ezshuttle recombination cloning expression vector pez lv199
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Ezshuttle Recombination Cloning Expression Vector Pez Lv199, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-Lv199+expression+vector+for+LxR+recombination+cloning/pm30332648-264-9-15
Average 94 stars, based on 1 article reviews
ezshuttle recombination cloning expression vector pez lv199 - by Bioz Stars, 2026-09
94/100 stars
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pez m68 vector  (Genecopoeia)
94
Genecopoeia pez m68 vector
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Pez M68 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M68+expression+vector+for+LxR+recombination+cloning/pmc07244012-167-20-22
Average 94 stars, based on 1 article reviews
pez m68 vector - by Bioz Stars, 2026-09
94/100 stars
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pez lv195 vector  (Genecopoeia)
94
Genecopoeia pez lv195 vector
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Pez Lv195 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-Lv195+expression+vector+for+LxR+recombination+cloning/pmc08431472-149-54-56
Average 94 stars, based on 1 article reviews
pez lv195 vector - by Bioz Stars, 2026-09
94/100 stars
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pez m35 vector  (Genecopoeia)
94
Genecopoeia pez m35 vector
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Pez M35 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M35+expression+vector+for+LxR+recombination+cloning/pmc08728313-220-14-16
Average 94 stars, based on 1 article reviews
pez m35 vector - by Bioz Stars, 2026-09
94/100 stars
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pez lv151 vector  (Genecopoeia)
94
Genecopoeia pez lv151 vector
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Pez Lv151 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-Lv151+expression+vector+for+LxR+recombination+cloning/10__1158_slash_1535___7163__mct___15___0114___t-59-3-16
Average 94 stars, based on 1 article reviews
pez lv151 vector - by Bioz Stars, 2026-09
94/100 stars
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pez m98 vector  (Genecopoeia)
94
Genecopoeia pez m98 vector
Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a <t>pEZ-M02</t> plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.
Pez M98 Vector, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+expression+vectors/pEZ-M98+expression+vector+for+LxR+recombination+cloning/pm36684389-90-11-13
Average 94 stars, based on 1 article reviews
pez m98 vector - by Bioz Stars, 2026-09
94/100 stars
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Image Search Results


Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a pEZ-M02 plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.

Journal: Oncoimmunology

Article Title: Radiation fosters dose-dependent and chemotherapy-induced immunogenic cell death

doi: 10.4161/onci.28518

Figure Lengend Snippet: Figure 2. Radiotherapy promotes calreticulin surface translocation. (A–C) TSA cells were stably transfected with a pEZ-M02 plasmid encoding a calreticulin (CRT) fusion protein with the modular HaloTag ® reporter,and endoplasmic reticulum targeting KDEL sequences (A, top panel) corresponding to the translation of a fusion CRT-HaloTag-KDEL protein (A, middle panel). CRT remains in the ER in non-stressed cells, whereas upon immunogenic cell death (ICD) CRT translocates to the cell surface (A, bottom panel). Externally localized CRT-HaloTag-KDEL is irreversible bound by membrane impermeable HaloTag ® Alexa Fluor 488 ligand activating its fluorescent properties that can then be detected via fluorescence microscopy or flow cytometry (A, middle and bottom panels). (B and C) pEZ-M02-CRT-HaloTag-KDEL transfected TSA cells were treated for 24 h with the indicated dosage of ionizing radiation (IR, delivered at time 0 h) were exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via cytoflourimetric analysis. (B) Representative histograms at each dose of IR. (C) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated IR dosage vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD.

Article Snippet: For CRT analysis, Turbofectin 8.0 (Origene) was used to transfect TSA cells with a pEZ-M02 expression vector containing the Mus musculus CRT-HaloTag-KDEL fusion construct (refer to ) following the manufacturer’s instructions (GeneCopoeia).

Techniques: Translocation Assay, Stable Transfection, Transfection, Plasmid Preparation, Fluorescence, Microscopy, Flow Cytometry, Irradiation

Figure 6. Calreticulin translocation to the cell surface in platinum and ionizing radiation treated TSA cells. (A and B) Externalization of calreticulin (CRT) was monitored using pEZ-M02-CRT-Halotag-KDEL stably transfected TSA breast cancer cells treated for 24 h with platinum ± the indicated dosage of ionizing radiation (IR, delivered at time 0 h) exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via fluorescence cytometry. ( A ) Representative histograms from the indicated treatment. ( B ) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated platinum agent ± IR vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD. Statistical analysis was performed by paired Student’s t test; P values < 0.05 were considered statistically significant.

Journal: Oncoimmunology

Article Title: Radiation fosters dose-dependent and chemotherapy-induced immunogenic cell death

doi: 10.4161/onci.28518

Figure Lengend Snippet: Figure 6. Calreticulin translocation to the cell surface in platinum and ionizing radiation treated TSA cells. (A and B) Externalization of calreticulin (CRT) was monitored using pEZ-M02-CRT-Halotag-KDEL stably transfected TSA breast cancer cells treated for 24 h with platinum ± the indicated dosage of ionizing radiation (IR, delivered at time 0 h) exposed to the impermeable HaloTag ® Alexa Fluor 488 ligand. The amount of green fluorescence indicative of cell surface CRT was detected via fluorescence cytometry. ( A ) Representative histograms from the indicated treatment. ( B ) Mean fluorescent intensity (MFI) detected from cells irradiated with the indicated platinum agent ± IR vs. non-irradiated cells normalized to 1. Shown are the MFI (n = 10 000 cells/group) ± SD. Statistical analysis was performed by paired Student’s t test; P values < 0.05 were considered statistically significant.

Article Snippet: For CRT analysis, Turbofectin 8.0 (Origene) was used to transfect TSA cells with a pEZ-M02 expression vector containing the Mus musculus CRT-HaloTag-KDEL fusion construct (refer to ) following the manufacturer’s instructions (GeneCopoeia).

Techniques: Translocation Assay, Stable Transfection, Transfection, Fluorescence, Cytometry, Irradiation