Journal: bioRxiv

Article Title: Tissue-wide profiling of human lungs reveals spatial sequestration of macrophages in tuberculosis

doi: 10.1101/2025.04.11.648467

Figure Lengend Snippet: (A) Heatmap showing the relative abundance of CD4 + (top) and CD8 + (bottom) T cell sub-populations in each lesion type. Rows are ordered based on the similarity in marker expression patterns. N, the border of necrotic lesions. (B) Representative OPAL multi-plex image illustrating a germinal center-like structure in a B3-type lesion. The image with merged DAPI, CD20, BCL6, and PNAd is shown. Arrows indicate PNAd + high endothelial venules and arrow heads indicate BCL6 + B cells. (C) Paired analysis comparing the spatial association relationships between B cells and Tfh (C10) or proinflammatory CD4 + T cells (C08) in all B3-type lesions. Each line indicates a lesion. The level of statistical significance is determined using Wilcoxon signed-rank test, **** P < 0.0001. (D) Cell type pheno-map of a representative B3-type lesion with cell type indicated. (E) Scatter plots showing the spatial centrality index (immCPI) of Tfh (C10), proinflammatory CD4 + T cells (C08), and proinflammatory CD8 + T cells (C11) in all B3-type lesions ( n = 110). The level of statistical significance is determined using a Mann-Whitney U test, **** P < 0.0001. (F) Heatmap showing the median value of pair-wised spatial association relationships between CD4 + and CD8 + T cell subsets in B3 (left) and T (right) type lesions. (G) Column scatter plot showing spatial relationship between macrophages and CD4 + and CD8 + T cell subtypes in T-type lesions. (H) Heatmap indicating statistical significance from post hoc multi-group comparison results in (G) using a One-way ANOVA test, the level of significance was set at P = 0.05.

Article Snippet: Next, tissue sections were incubated for 1 hour at room temperature with primary antibodies against CD20 (1:100, L26, Abcam), CD4 (1:100, EPR6855, Abcam), and overnight under 4°C with BCL6 (1:100, EPR11410-43, Abcam), and PNAd (1:200, MECA-79, Novus Biologicals).

Techniques: Marker, Expressing, MANN-WHITNEY, Comparison

Journal: Cell Reports

Article Title: Fiber deprivation and microbiome-borne curli shift gut bacterial populations and accelerate disease in a mouse model of Parkinson’s disease

doi: 10.1016/j.celrep.2023.113071

Figure Lengend Snippet:

Article Snippet: The following antibodies were used: monoclonal rabbit anti-pS129-αSyn (Abcam, ab51253; 1:1000), monoclonal mouse anti-pS129-αSyn (Prothena Biosciences Inc., 11A5; 1:1000), polyclonal chicken anti-tyrosine hydroxylase (Abcam, ab76442; 1:1000), polyclonal rabbit anti-tyrosine hydroxylase (Merck (Sigma-Aldrich), AB152; 1:1000), polyclonal rat anti-dopamine transporter (MAB369, Merck (Sigma-Aldrich); 1:1000), polyclonal rabbit pan αSyn (S3062, Sigma-Aldrich, 1:1000), monoclonal mouse human αSyn 211 clone (S5566, Sigma-Aldrich, 1:1000) and rabbit anti-ionized calcium-binding adapter molecule 1 (Iba1) (1919741, Wako, 1:1000).

Techniques: Control, Plasmid Preparation, Recombinant, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Amplification, Sequencing, Software

Journal: iScience

Article Title: Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells

doi: 10.1016/j.isci.2021.103605

Figure Lengend Snippet:

Article Snippet: Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] , Abcam , #ab220074.

Techniques: Produced, Recombinant, Enzyme-linked Immunosorbent Assay, Adhesive, Western Blot, CRISPR, Control, Plasmid Preparation, Software