Journal: Biosensors & bioelectronics

Article Title: A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability.

doi: 10.1016/j.bios.2017.04.044

Figure Lengend Snippet: Figure 2. A). Biotinylated PtNPs captured by streptavidin conjugated magnetic beads

Article Snippet: Human Kallikrein 3/PSA Biotinylated Antibody, Human Kallikrein 3/PSA Antibody, recombinant Human Kallikrein 3/PSA Protein, Human C-Reactive Protein/CRP Biotinylated Antibody, Human C-Reactive Protein/CRP Antibody, and recombinant Human C-Reactive Protein/CRP Protein were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Magnetic Beads

Journal: Biosensors & bioelectronics

Article Title: A fully integrated distance readout ELISA-Chip for point-of-care testing with sample-in-answer-out capability.

doi: 10.1016/j.bios.2017.04.044

Figure Lengend Snippet: Figure 4. A). Distance change with increasing concentrations of biotinylated PtNPs.

Article Snippet: Human Kallikrein 3/PSA Biotinylated Antibody, Human Kallikrein 3/PSA Antibody, recombinant Human Kallikrein 3/PSA Protein, Human C-Reactive Protein/CRP Biotinylated Antibody, Human C-Reactive Protein/CRP Antibody, and recombinant Human C-Reactive Protein/CRP Protein were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques:

Journal: Oncogene

Article Title: α3β1 integrins regulate CD151 complex assembly and membrane dynamics in carcinoma cells within 3D environments.

doi: 10.1038/onc.2012.415

Figure Lengend Snippet: Figure 6. a3 integrin promotes protrusion in cells in 3D ECM. (a) Representative images of cells expressing control or a3 integrin shRNA were co-transfected with Lifeact–GFP (LA–GFP) and CD151–RFP and embedded in 3D native collagen I (COL I) or matrigel (MG) within imaging chambers. Cells were left to invade for 24 h and were then fixed and subjected to confocal microscopy. (b) Representative confocal z-stack projections of control cells expressing Lifeact–GFP and embedded within cy3-labelled COL or MG 3D gels. (c) Representative 3D projections of confocal z-stacks of control or a3 integrin shRNA cells expressing Lifeact–GFP and invading into 3D COL or MG gels. Images are stills taken from movies shown in Supplementary data. (d) Analysis of dynamics of Lifeact–GFP protrusions from cells as shown in example images in c. Protrusion number per cell was calculated for each time point over the duration of the movie (d). n ¼ 8 cells per condition over three independent experiments. (e) Graph showing change in protrusion volume as a % of total cell volume over time from analysis of movies as in (d). (f) Protrusion: retraction ratio as calculated from protrusion dynamics data from live cell analysis as in d and e. *P ¼ 0.01, **P ¼ 0.005 compared with shCon. Scale bars, 10 mm.

Article Snippet: 1 ml of diluted collagen or laminin was added to an aliquot of Cy3 label from a Cy3 Mono-Reactive Dye Pack (GE Healthcare, Bucks, UK) and mix/rotated for 30 min at 41C in the dark.

Techniques: Expressing, Control, shRNA, Transfection, Imaging, Confocal Microscopy, Cell Analysis

Journal: International Journal of Molecular Sciences

Article Title: Transcriptome-Based WGCNA Reveals Hub Genes Involved in Copper Resistance of Penicillium janthinellum GXCR

doi: 10.3390/ijms27073290

Figure Lengend Snippet: Effect of Cu 2+ on the antioxidant enzyme activity of the three strains ( a ) The influence of Cu 2+ on the content of reactive oxygen species in the three strains. ( b ) The influence of Cu 2+ on the SOD activity of the three strains. ( c ) The influence of Cu 2+ on the POD activity of the three strains. ( d ) The influence of Cu 2+ on the CAT activity of the three strains. Note: Compare EC-6 and UC-8 with WT. Significant differences (* p < 0.05; ** p < 0.01).

Article Snippet: Reactive oxygen species (ROS) within bacterial cells were detected using the Solarbio Reactive Oxygen Species Assay Kit (Beijing Solarbio Technology Co., Ltd., Beijing, China), while superoxide dismutase (SOD) within bacterial cells was detected using the Superoxide Dismutase Assay Kit (Beijing Solarbio Technology Co., Ltd., Beijing, China).

Techniques: Activity Assay

Journal: JHEP Reports

Article Title: Myeloid TGF-β signaling shapes liver macrophage heterogeneity and metabolic liver disease pathogenesis

doi: 10.1016/j.jhepr.2025.101488

Figure Lengend Snippet: Tgfbr1 ablation triggers the induction of proinflammatory and senescent macrophages in liver. (A) Feature plots illustrating Pycard, Casp1, Nlrp3, and Il1b mRNA expression within macrophage subclusters. (B) qPCR analysis of gene expression in cultured liver macrophages isolated from control and MKO MASH livers treated with PBS (n = 3) or LPS (5 ng/ml) plus IFN-γ (5 ng/ml) (n = 3). (C) Immunoblots of total protein lysates from cultured liver macrophages. Primary macrophages isolated from control and MKO mouse livers were treated with PBS (n = 2) or LPS (200 ng/ml) (n = 2) for 3 h followed by ATP (3 mM) treatment for 30 min. (D) Cytokine concentrations in conditioned media from cultured liver macrophages treated with PBS (n = 4) or LPS (200 ng/ml) (n = 4) for 3 h followed by ATP (3 mM) stimulation for 30 min. Data in (B and D) represent mean ± SEM; analyzed by one-way ANOVA with post hoc analysis using Tukey’s test. IFN-γ, interferon-γ; LPS, lipopolysaccharide; MKO, myeloid-specific knockout; MASH, metabolic dysfunction-associated steatohepatitis; qPCR, quantitative PCR.

Article Snippet: Antibodies against IL-1β, cleaved IL-1β, caspase 1, cleaved caspase 1, and NLRP3 (contained in a mouse reactive inflammasome antibody sampler kit; catalog #20836), as well as p16 INK4A and p21 Waf1/Cip1 (contained in Senescence Marker Antibody Sampler Kit; catalog #56062) were purchased from Cell Signaling Technology.

Techniques: Expressing, Gene Expression, Cell Culture, Isolation, Control, Western Blot, Knock-Out, Real-time Polymerase Chain Reaction