reaction buffer Search Results


thermopol buffer  (New England Biolabs)
98
New England Biolabs thermopol buffer
Thermopol Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/ThermoPol+Reaction+Buffer/us07449297-443-27-26
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cuaac kit  (Jena Bioscience)
93
Jena Bioscience cuaac kit
Cuaac Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/CuAAC+Biomolecule+Reaction+Buffer+Kit/pm40550823-255-13-17
Average 93 stars, based on 1 article reviews
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b0202s  (New England Biolabs)
99
New England Biolabs b0202s
B0202s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/T4+DNA+Ligase+Reaction+Buffer/10__1038_slash_protex__2010__207-90-87-86
Average 99 stars, based on 1 article reviews
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nebnext ultra ii end prep reaction buffer  (New England Biolabs)
95
New England Biolabs nebnext ultra ii end prep reaction buffer
Nebnext Ultra Ii End Prep Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/NEBNext+End+Repair+Reaction+Buffer/10__1111_slash_oik__10880-139-16-16
Average 95 stars, based on 1 article reviews
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t4 rna ligase buffer  (New England Biolabs)
97
New England Biolabs t4 rna ligase buffer
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
T4 Rna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/T4+RNA+Ligase+Reaction+Buffer/bio_rxiv__64898__2026__04__14__718460-349-13-36
Average 97 stars, based on 1 article reviews
t4 rna ligase buffer - by Bioz Stars, 2026-11
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nebnext quick ligation reaction buffer  (New England Biolabs)
98
New England Biolabs nebnext quick ligation reaction buffer
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
Nebnext Quick Ligation Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/NEBNext+Quick+Ligation+Reaction+Buffer/pmc12955425-82-17-17
Average 98 stars, based on 1 article reviews
nebnext quick ligation reaction buffer - by Bioz Stars, 2026-11
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q5 reaction buffer 5  (New England Biolabs)
99
New England Biolabs q5 reaction buffer 5
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
Q5 Reaction Buffer 5, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/Q5+Reaction+Buffer/pmc11707876-102-20-10
Average 99 stars, based on 1 article reviews
q5 reaction buffer 5 - by Bioz Stars, 2026-11
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dedicated buffer  (New England Biolabs)
96
New England Biolabs dedicated buffer
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
Dedicated Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/DNase+I+Reaction+Buffer/pm42764332-594-20-23
Average 96 stars, based on 1 article reviews
dedicated buffer - by Bioz Stars, 2026-11
96/100 stars
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10x phosphorylation reaction buffer  (New England Biolabs)
97
New England Biolabs 10x phosphorylation reaction buffer
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
10x Phosphorylation Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/T4+Polynucleotide+Kinase+Reaction+Buffer/bio_rxiv__64898__2026__04__30__722071-422-20-24
Average 97 stars, based on 1 article reviews
10x phosphorylation reaction buffer - by Bioz Stars, 2026-11
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cuaac biomolecule reaction buffer kit  (Jena Bioscience)
91
Jena Bioscience cuaac biomolecule reaction buffer kit
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
Cuaac Biomolecule Reaction Buffer Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/CuAAC+Biomolecule+Reaction+Buffer+Kit/bio_rxiv__693374-207-5-12
Average 91 stars, based on 1 article reviews
cuaac biomolecule reaction buffer kit - by Bioz Stars, 2026-11
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antarctic phosphatase  (New England Biolabs)
96
New England Biolabs antarctic phosphatase
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
Antarctic Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/Antarctic+Phosphatase+Reaction+Buffer/us10435711-279-20-22
Average 96 stars, based on 1 article reviews
antarctic phosphatase - by Bioz Stars, 2026-11
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cuaac solutions  (Jena Bioscience)
93
Jena Bioscience cuaac solutions
a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and <t>T4</t> <t>RNA</t> ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.
Cuaac Solutions, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer/CuAAC+Cell+Reaction+Buffer+Kit/bio_rxiv__2022__10__27__514014-122-1-3
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Image Search Results


a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and T4 RNA ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.

Journal: bioRxiv

Article Title: Ultra-sensitive FLORA-seq links cell-type-specific tRNAome dynamics to differentiation trajectories guiding therapeutic suppressor tRNA candidate selection

doi: 10.64898/2026.04.14.718460

Figure Lengend Snippet: a, Cloverleaf structure of tRNA with some modifications. m 1 A, 1-methyladenosine; m 1 G, 1-methylguanosine; acp 3 U, 3-(3-amino-3-carboxypropyl) uridine; m G, N , N -dimethylguanosine; m 3 C, 3-methylcytidine; ncm 5 U, 5-carbamoylmethyluridine; mcm 5 U, 5-methoxycarbonylmethyluridine; mcm 5 s U, 5-methoxycarbonylmethyl-2-thiouridine; I, inosine; i A, N -isopentenyladenosine; t A, N -threonylcarbamoyladenosine; m t A, N -methyl- N -threonylcarbamoyladenosine; ms t A, 2-methylthio- N -threonylcarbamoyladenosine; m 1 I, 1-methylinosine. b, Chemical structures of some tRNA modifications. c, The ligation efficiency of Poly(A) polymerase and T4 RNA ligase 2. Using an equal amount of total tRNA without any adapter ligation or poly(A) addition as a control, the ligation efficiency is calculated as the ratio of RNA molecules with successfully added adapters or poly(A) tails to those in the control. d, A pool of RNA oligonucleotides with identical sequences but varying 5’-terminal nucleotides (A, C, G, or U) was subjected to the template-switching reverse transcription library preparation under optimized conditions. The bar graph shows the proportion of each oligonucleotide recovered in the final sequencing libraries. e, Schematic overview illustrating the steps required for tRNA library preparation using FLORA-seq. f, Raw read coverage of mito_tRNA Leu (TAA) and cyto_tRNA Leu (AAG) from 20 HEK 293T. g, Raw read coverage of mito_tRNA Leu (TAG) and cyto_tRNA Leu (TAG) from 5 mouse morulae. h, Raw read coverage of 3’ and 5’tdR from 5 mouse morulae. i, Raw read coverage of internal tdRs from 5 mouse morulae. j, Pie chart illustrating the distribution of distinct RNA species identified from non-ribosomal genome-aligned reads in mouse morula sequencing data. Reads with mapped lengths below 15 bp were excluded from the analysis.

Article Snippet: The ligation reaction was performed in a 20 μL reaction containing 1 × T4 RNA ligase buffer, 200 ng tRNA, 10 μM pre-adenylated DNA adapter (Synthesized by Novazyme), and 200 U of T4 RNA ligase 2 (NEB) at 25°C for 2 h

Techniques: Ligation, Adapter Ligation, Control, Reverse Transcription, Sequencing