Journal: Scientific Reports

Article Title: The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation

doi: 10.1038/s41598-017-06969-4

Figure Lengend Snippet: Gene expression of RGC markers in NMDA-injured mouse retinas with and without hesperidin treatment. The transcriptional levels of Rbpms ( a ), Brn3b ( b ) and Brn3c ( c ) were compared in the eyes of mice treated with PBS without NMDA (white bars), treated with PBS (black bars), and treated with hesperidin (gray bars) 24 h after NMDA injection. The gene expression of each RGC marker was normalized to Gapdh. Expression levels are shown as percentages of the average expression in eyes treated with PBS without NMDA. Data represent mean ± SD (Rbpms: n = 14, Brn3b, Brn3c: n = 6 each). *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Predesigned primers and probes, purchased from Life Tec, were used as follows, with the relevant genes in italics: Pou4f2 (Mm00454754_s1), Pou4f3 (Mm04213795_s1), and Rbpms (Mm00803908_m1) , TNF-1a (Mm00441883_g1), Ddit3 (Mm00492097_m1) and Gapdh (Mm01256744_m1).

Techniques: Gene Expression, Injection, Marker, Expressing

Journal: Scientific Reports

Article Title: The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation

doi: 10.1038/s41598-017-06969-4

Figure Lengend Snippet: Hesperidin treatment preserved RGC marker-positive cells after NMDA injury. Representative photographs at lower ( a – c ) and higher ( d – l ) magnification with nuclear staining (DAPI) and immunostaining with anti-RBPMS antibody, taken 24 h after intravitreal injection with PBS ( a , d , g , j ), 30 nmol NMDA ( b , e , h , k ), or NMDA with hesperidin ( c , f , i , l ). RBPMS-positive cells in the GCL are shown by the arrows. ( m ) Histogram showing the average number of RBPMS-positive cells in the retinas. Data represent mean ± SD, (vehicle, n = 6, NMDA, n = 8, NMDA with hesperidin, n = 7) ***p < 0.001. A-C scale bar: 500 µm, D-L scale bar: 50 µm. GCL; ganglion cell layer, INL; inner nuclear layer, ONL; outer nuclear layer.

Article Snippet: Predesigned primers and probes, purchased from Life Tec, were used as follows, with the relevant genes in italics: Pou4f2 (Mm00454754_s1), Pou4f3 (Mm04213795_s1), and Rbpms (Mm00803908_m1) , TNF-1a (Mm00441883_g1), Ddit3 (Mm00492097_m1) and Gapdh (Mm01256744_m1).

Techniques: Marker, Staining, Immunostaining, Injection

Journal: iScience

Article Title: Transplanted human induced pluripotent stem cells- derived retinal ganglion cells embed within mouse retinas and are electrophysiologically functional

doi: 10.1016/j.isci.2022.105308

Figure Lengend Snippet:

Article Snippet: anti-RBPMS Alexa Fluor 647 , Novus Biologicals , Cat#: NBP273835AF647.

Techniques: Virus, Recombinant, RNA Sequencing, Software