Journal: Molecules

Article Title: Preparative Isolation and Purification of Lignans from Justicia procumbens Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode

doi: 10.3390/molecules20047048

Figure Lengend Snippet: HPLC chromatograms. ( A ) Crude sample from J. procumbens ; ( B ) HSCCC fraction of peak 1 in ; ( C ) HSCCC fraction of peak 2 in ; ( D ) HSCCC fraction of peak 3 in ; ( E ) HSCCC fraction of peak 4 in . Conditions: YMC-Pack ODS-A column (150 mm × 4.6 mm I.D., 5 µm); column temperature, 25 °C; mobile phase, methanol and water at the gradient (methanol: 0–60 min, 10%–90%; 60–70 min, 90%); flow rate, 0.8 mL/min; detection, 254 nm.

Article Snippet: Raw material samples of J. procumbens were purchased from Jinhua Pharmaceutical co., LTD. (Jinhua, China.)

Techniques: High Speed Counter-current Chromatography

Journal: Molecules

Article Title: Preparative Isolation and Purification of Lignans from Justicia procumbens Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode

doi: 10.3390/molecules20047048

Figure Lengend Snippet: HSCCC chromatogram of the crude extract from J. procumbens using stepwise elution with solvent systems A and B. Solvent system A: n -hexane-ethyl acetate-methanol-water (1.3:1:1.3:1, v/v/v/v), solvent system B: n -hexane-ethyl acetate-methanol-water (2.5:1:2.5:1, v/v/v/v); stationary phase: upper phase of solvent system A; mobile phase: lower aqueous phase of solvent system A and B; column capacity, 320 mL; rotation speed, 1000 rpm; column temperature, 25 °C; flow rate, 3.0 mL/min; detection, 254 nm; sample injected, 300 mg in 5 mL upper phase and 5 mL lower phase; retention of the stationary phase, 56%; separation mode: head-to-tail. Peaks: justicidin B ( 1 ), justicidin A ( 2 ), 6'-hydroxyjusticidin C ( 3 ) and lignan J 1 ( 4 ).

Article Snippet: Raw material samples of J. procumbens were purchased from Jinhua Pharmaceutical co., LTD. (Jinhua, China.)

Techniques: High Speed Counter-current Chromatography, Injection