Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effect of Electroacupuncture on Activation of p38MAPK in Spinal Dorsal Horn in Rats with Complete Freund's Adjuvant-Induced Inflammatory Pain

doi: 10.1155/2012/568273

Figure Lengend Snippet: Effect of EA on phosphor-p38MAPK-immunoreactivty (p-p38MAPK-IR) expression in the ipsilateral spinal dorsal horn induced in CFA rats. (a) Representative sections of p-p38MAPK-IR cells in the ipsilateral spinal dorsal horn at postinjection days 3 and 14. (section 1, saline group; sections 2 and 4, CFA group, sections 3 and 5, CFA + EA group; sections 2 and 3, postinjection day 3; sections 4 and 5, postinjection day 14; Bars = 50 μ m; section thickness = 30 μ m). (b) Quantification of p-p38MAPK-IR showing that EA suppressed p-p38MAPK-IR expression in the ipsilateral spinal dorsal horn. Values are mean ± SD, n = 3 animals per experimental group. ** P < 0.01 versus saline group; ▲▲ P < 0.01 versus CFA group at corresponding time points.

Article Snippet: Sections were blocked in 5% normal goat serum for 30 min at room temperature and then transferred to primary antibody solution containing rabbit anti rat p-p38MAPK (1 : 200, CST, USA) for overnight at 4°C.

Techniques: Expressing, Saline

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Baicalin Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension to Improve Hypoxic Cor Pulmonale by Reducing the Activity of the p38 MAPK Signaling Pathway and MMP-9

doi: 10.1155/2016/2546402

Figure Lengend Snippet: Expression of p-p38 in pulmonary arterioles and lung tissue. (a, b) p-p38 was expressed more abundantly in pulmonary arteriole cell nuclei in the hypoxia group than in the baicalin group and the control group, as visualized by immunohistochemistry. ( n = 8/group) ## indicates P < 0.01 compared to the control group and ∗ indicates P < 0.05 compared to the hypoxia group. (c, d) Western blot analysis of p-p38 expression in the lung showed that p-p38 expression was higher in the hypoxia group than in the control group and that this increased expression was attenuated by baicalin treatment. Representative blots are shown. ( n = 8/group) ## indicates P < 0.01 compared to the control group and ∗∗ indicates P < 0.01 compared to the hypoxia group.

Article Snippet: The rabbit anti-rat p-p38 MAPK polyclonal antibody (#4631) was obtained from Cell Signaling Technology (USA).

Techniques: Expressing, Control, Immunohistochemistry, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: The MAPK signaling pathway mediates the GPR91-dependent release of VEGF from RGC-5 cells

doi: 10.3892/ijmm.2015.2195

Figure Lengend Snippet: Succinate-induced activation of ERK1/2, JNK and p38 MAPK signaling pathways in RGC-5 cells. (A) Western blot analysis of ERK1/2, JNK and p38 MAPK phosphorylation in RGC-5 cells incubated with various concentrations of succinate for 10 min. (B) Quantitative analysis of the band density. Each column represents the mean ± SD (n=3). (C) Western blot analysis of ERK1/2, JNK and p38 MAPK phosphorylation in RGC-5 cells treated with 10 µ M of succinate for different periods of time. (D) Quantitative analysis of the band density. Each column represents the mean ± SD (n=3). (E) Immunofluorescence images showing ERK1/2, JNK and p38 MAPK in the cytoplasm of RGC-5 cells treated with 10 µ M succinate for 10 min. ** P<0.01 vs. the untreated control. Scale bar, 50 µ m.

Article Snippet: The cells were blocked in 5% BSA for 1 h at room temperature, followed by overnight incubation at 4°C with the primary antibodies: rabbit anti-rat GPR91 polyclonal antibody (1:200; NBP1-00861; Novus Biologicals LLC, Littleton, CO, USA), rabbit anti-rat p-ERK1/2 monoclonal antibody (1:200; #4370), rabbit anti-rat p-JNK monoclonal antibody (1:200; #4668) and rabbit anti-rat p-p38 monoclonal antibody (1:200; #4511; Cell Signaling Technology, Boston, MA, USA).

Techniques: Activation Assay, Protein-Protein interactions, Western Blot, Phospho-proteomics, Incubation, Immunofluorescence, Control

Journal: International Journal of Molecular Medicine

Article Title: The MAPK signaling pathway mediates the GPR91-dependent release of VEGF from RGC-5 cells

doi: 10.3892/ijmm.2015.2195

Figure Lengend Snippet: G-protein-coupled receptor 91 (GPR91) modulates succinate-induced ERK1/2 and JNK signaling in RGC-5 cells. (A) Changes in ERK1/2, JNK and p38 MAPK phosphorylation (determined by western blot analysis) in RGC-5 cells transduced with LV.shScrambled or LV. shGPR91. (B) Quantitative analysis of the band density. Each column represents the mean ± SD (n=3). (C) Effect of the JNK inhibitor, SP600125, on ERK1/2 phosphorylation in RGC-5 cells treated with succinate. (D) Quantitative analysis of the band density. Each column represents the mean ± SD (n=3). (E) Effect of the ERK1/2 inhibitor, U0126, on JNK phosphorylation in RGC-5 cells treated with succinate. (F) Quantitative analysis of the band density. Each column represents the mean ± SD (n=3). ** P<0.01 vs. the untreated control. ## P<0.01 vs. LV.shScrambled-transduced cells.

Article Snippet: The cells were blocked in 5% BSA for 1 h at room temperature, followed by overnight incubation at 4°C with the primary antibodies: rabbit anti-rat GPR91 polyclonal antibody (1:200; NBP1-00861; Novus Biologicals LLC, Littleton, CO, USA), rabbit anti-rat p-ERK1/2 monoclonal antibody (1:200; #4370), rabbit anti-rat p-JNK monoclonal antibody (1:200; #4668) and rabbit anti-rat p-p38 monoclonal antibody (1:200; #4511; Cell Signaling Technology, Boston, MA, USA).

Techniques: Phospho-proteomics, Western Blot, Transduction, Control