rat cdna library Search Results


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Becton Dickinson rat liver marathon-ready cdna library
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Johns Hopkins HealthCare hippocampal cdna library
Hippocampal Cdna Library, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rat Liver Cdna Library, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dawley Inc random-primed cdna library from sprague dawley rat hippocampus
Random Primed Cdna Library From Sprague Dawley Rat Hippocampus, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dawley Inc sprague dawley rat enterocyte cdna library
Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal <t>cDNA</t> library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Sprague Dawley Rat Enterocyte Cdna Library, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Seegene Technologies rat skeletal muscle full-length cdna library
Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal <t>cDNA</t> library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Rat Skeletal Muscle Full Length Cdna Library, supplied by Seegene Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cold Spring Harbor Laboratory Meetings retroviral marx rat cdna library
Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal <t>cDNA</t> library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Retroviral Marx Rat Cdna Library, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amersham Life Sciences Inc rat liver cdna library
Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal <t>cDNA</t> library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Rat Liver Cdna Library, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Wolters Kluwer Health rat or mouse outer medulla cdna library
Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal <t>cDNA</t> library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Rat Or Mouse Outer Medulla Cdna Library, supplied by Wolters Kluwer Health, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioChain Institute rat heart cdna library
Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal <t>cDNA</t> library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.
Rat Heart Cdna Library, supplied by BioChain Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal cDNA library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.

Journal: BMC Cardiovascular Disorders

Article Title: The rat STSL locus: characterization, chromosomal assignment, and genetic variations in sitosterolemic hypertensive rats

doi: 10.1186/1471-2261-3-4

Figure Lengend Snippet: Alternative splicing of Abcg8 mRNA expression in SD, SHR and WKY rats by RT-PCR Panel A shows the gene organisation of Abcg8 , and below it the organisation of an alternatively spliced form detected by sequencing clones from a rat intestinal cDNA library (see Text). Panel B shows that the effect of the inclusion of exon 2b in the mRNA results in an in-frame addition of 22 amino acids. Total RNAs were prepared from liver and small intestine from SD, SHR and WKY rats maintained on a standard rodent chow diet and analysed by RT-PCR with primers (see Methods) based outside the alternatively spliced exon 2b. Direct amplification of a pooled cDNA library sample indicated a major DNA product of 396 bp, with a minor product of 462 bp, containing exon 2b. DNA products, using cloned wild type Abcg8 cDNA (wt) and alternatively spliced cDNA (AS) are shown in tracks 3 and 4 respectively. As can be seen, liver and intestine RNA from all three strains show a minor DNA product of 462 bp (confirmed by sequencing) contained the insertion of exon2b sequences.

Article Snippet: Rat cDNAs for Abcg5 and Abcg8 were identified previously by amplification of these sequences from a Sprague Dawley rat enterocyte cDNA library, using primers located in areas conserved between mouse and human (Genbank AF312714 and AF351785 respectively).

Techniques: Alternative Splicing, Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Clone Assay, cDNA Library Assay, Amplification

Protein Expression of Abcg5 in liver total membrane preparations. Membrane preparations (20 μg protein) from rat tissues were isolated and analysed by SDS-PAGE, blotted and probed with anti- Abcg5 antiserum (R116, panel A), probed with anti-transferrin antiserum (panel B) or the gel was fixed and stained with Coomassie Brilliant Blue R-250 (panel C). Lane 1 contains a recombinant E. coli expressed N-terminal fragment of sterolin-1 as a control for antibody binding, lanes 2 and 3 contain Sprague-Dawley (SD) spleen membrane extracts. Liver from Wistar (lanes 4 and 5), SHR (lanes 6 and 7) and Sprague-Dawley (SD, lanes 8 and 9) Densitometry measurements when normalised to transferrin show a 2-fold increase in sterolin-1 protein in SHR rat liver samples, compared to SD and Wistar rat liver. No antibody for sterolin-2 is currently available.

Journal: BMC Cardiovascular Disorders

Article Title: The rat STSL locus: characterization, chromosomal assignment, and genetic variations in sitosterolemic hypertensive rats

doi: 10.1186/1471-2261-3-4

Figure Lengend Snippet: Protein Expression of Abcg5 in liver total membrane preparations. Membrane preparations (20 μg protein) from rat tissues were isolated and analysed by SDS-PAGE, blotted and probed with anti- Abcg5 antiserum (R116, panel A), probed with anti-transferrin antiserum (panel B) or the gel was fixed and stained with Coomassie Brilliant Blue R-250 (panel C). Lane 1 contains a recombinant E. coli expressed N-terminal fragment of sterolin-1 as a control for antibody binding, lanes 2 and 3 contain Sprague-Dawley (SD) spleen membrane extracts. Liver from Wistar (lanes 4 and 5), SHR (lanes 6 and 7) and Sprague-Dawley (SD, lanes 8 and 9) Densitometry measurements when normalised to transferrin show a 2-fold increase in sterolin-1 protein in SHR rat liver samples, compared to SD and Wistar rat liver. No antibody for sterolin-2 is currently available.

Article Snippet: Rat cDNAs for Abcg5 and Abcg8 were identified previously by amplification of these sequences from a Sprague Dawley rat enterocyte cDNA library, using primers located in areas conserved between mouse and human (Genbank AF312714 and AF351785 respectively).

Techniques: Expressing, Membrane, Isolation, SDS Page, Staining, Recombinant, Control, Binding Assay