Journal: Cell reports

Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice

doi: 10.1016/j.celrep.2025.116841

Figure Lengend Snippet: Representative images and percent area densities of (A) CD68, (B) Iba1, (C) Gfap, (D) Clec7a, and (E) Tmem119 in the hippocampus and entorhinal/piriform cortical area (EC/PC area) of 9.5-month-old male PCfD mice treated with OIL or TAM at 3 months of age ( n = 16–20 per group). Scale bar: 500 μm. Mann-Whitney tests were used to determine statistical significance (CD68 hippocampus, p = 0.0222). Data are represented as the mean ± SEM. * p < 0.05.

Article Snippet: The primary antibodies were diluted as follows: rabbit- anti -Iba1 (FUJIFILM Wako, 019–19741, 1:500), rat- anti -CD68 (BioRad, MCA1957, 1:500), and guinea pig anti-Plin2 (Progen, GP40, 1:200).

Techniques: MANN-WHITNEY

Journal: Cell reports

Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice

doi: 10.1016/j.celrep.2025.116841

Figure Lengend Snippet: (A) Left: illustration showing approximate locations of the z stacks acquired for the neutral lipid imaging experiments. This image was generated using BioRender. Right: representative 3D full-thickness Imaris renderings of Iba1, CD68, and BODIPY co-stains of PCfD TAM and OIL hippocampi. All channels were masked outside of Iba1 surfaces to promote visualization of cells throughout the entire stack. Scale bar: 25 μm. Inset scale bar: 10 μm. (B) Quantification of Iba1-CD68-BODIPY colocalized volumes per total Iba1 volume. Significance was determined with a Mann-Whitney test ( n = 17–20 per group, p = 0.015). Data are represented as the mean ± SEM. (C) Volcano plot of lipid species altered between PCfD TAM and OIL groups. Statistically significantly altered species are colored ( n = 10 per group). (D) Heatmap of lipid species extracted from bulk posterior cortices of 9.5-month-old male mice via liquid chromatography-mass spectrometry (LC-MS). * p < 0.05.

Article Snippet: The primary antibodies were diluted as follows: rabbit- anti -Iba1 (FUJIFILM Wako, 019–19741, 1:500), rat- anti -CD68 (BioRad, MCA1957, 1:500), and guinea pig anti-Plin2 (Progen, GP40, 1:200).

Techniques: Imaging, Generated, MANN-WHITNEY, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Journal: Cell reports

Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice

doi: 10.1016/j.celrep.2025.116841

Figure Lengend Snippet: (A) Volcano plot of lipid species altered between CD11b high /CD45 int brain myeloid cells from male and female 9.5-month-old WT and PS19 mice (without floxed DGAT alleles) previously gavaged with tamoxifen at 3 months of age. Statistically significantly altered species are colored ( n = 13–14 per group, sexes combined). (B) Representative high-resolution (100×) image of Iba1, BODIPY, CD68, and Plin2 immunofluorescent stains in the hippocampus of a 9-month-old male standard PS19 mouse without floxed DGAT alleles or any treatments. Scale bar: 10 μm. We observed this general staining pattern at 63× in dozens of PS19 and WT mice of multiple strain backgrounds, including PCfD mice treated with TAM and OIL. (C) Representative TEM images of brain myeloid cells from dentate gyrus granule cell layers of 10-month-old male WT and PS19 mice. Myeloid cells were identified by the presence of heterochromatin around the edges of irregularly shaped nuclei. Left: low-magnification images (3,000×, scale bar: 2 μm). Right: high-magnification views (15,000×, scale bar: 400 nm) of lipid-laden lysosomes highlighted in the dashed squares. The red arrows indicate single lysosomal membranes, and the white arrows indicate double lysosomal membranes associated with lipid accumulation. Images are representative of what we have observed from reviewing 6–8 images per sample from 3 PS19 and 2 WT male mice.

Article Snippet: The primary antibodies were diluted as follows: rabbit- anti -Iba1 (FUJIFILM Wako, 019–19741, 1:500), rat- anti -CD68 (BioRad, MCA1957, 1:500), and guinea pig anti-Plin2 (Progen, GP40, 1:200).

Techniques: Staining

Journal: Biomedicines

Article Title: Resveratrol and Its Analogue 4,4′-Dihydroxy-trans-stilbene Inhibit Lewis Lung Carcinoma Growth In Vivo through Apoptosis, Autophagy and Modulation of the Tumour Microenvironment in a Murine Model

doi: 10.3390/biomedicines10081784

Figure Lengend Snippet: ( a , b ) Representative images of CD163 immunostaining in vehicle, DHS, and RSV tumour masses (scale bars = 100 μm; 20× magnification ( a )) and in vehicle sample (scale bar 50 μm; 100× magnification ( b )); ( c ) Quantitative analysis of CD163, CD68 and CD3 positive cells/sample (* p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: The primary antibodies used for immunohistochemistry (IHC) were the following: anti-PCNA mouse monoclonal antibody (PC10, Dako, Agilent Technologies, Santa Clara, CA, USA, 1:200), anti-CD31 rat monoclonal antibody (e-Bioscience, Thermo Fisher Scientific, Waltham, MA, USA, 1:400), anti-CD163 RabMab rabbit mAb (Abcam, Cambridge, UK, Clone:EPR19518, 1:500), anti-CD3 rabbit polyclonal antibody (Origene, Rockville, MD, USA, TA354250, 1:200) and anti-CD68 rat monoclonal antibody (Origene, Rockville, MD, USA, SM1550PS, 1:200) were used.

Techniques: Immunostaining

Journal: PLoS ONE

Article Title: Effects of prenatal low protein and postnatal high fat diets on visceral adipose tissue macrophage phenotypes and IL-6 expression in Sprague Dawley rat offspring

doi: 10.1371/journal.pone.0169581

Figure Lengend Snippet: A-I) ATMs were stained with anti-CD68 antibody. The number of ATMs was counted per 1,000 adipocytes area. Three randomly selected areas were counted for each rat. Average number of the ATMs was used for further statistical analysis. Number of CD68 positive cells in each group is normalized to the number of CD68 + macrophages in NP+NE group. Data are presented as mean ± SEM, n = 6–9.”#” P<0.05 compared to NE; “*” P<0.05 compared to NP. E, F, G, and H are enlargements of the framed fields of A, B, C, and D, respectively. Magnificence of A-D: 200X; E-H 400X.

Article Snippet: Then SVCs were incubated with anti-rat CD68 FITC (Acris-antibodies, San Diego, CA) for 30 minutes at 4°C in dark and washed with Magnetic Cell Separation (MACS) buffer (Miltenyi Biotec Inc., Auburn, CA).

Techniques: Staining

Journal: PLoS ONE

Article Title: Effects of prenatal low protein and postnatal high fat diets on visceral adipose tissue macrophage phenotypes and IL-6 expression in Sprague Dawley rat offspring

doi: 10.1371/journal.pone.0169581

Figure Lengend Snippet: P value of Two-way ANOVA for LP and HE on ATM phenotype ^a .

Article Snippet: Then SVCs were incubated with anti-rat CD68 FITC (Acris-antibodies, San Diego, CA) for 30 minutes at 4°C in dark and washed with Magnetic Cell Separation (MACS) buffer (Miltenyi Biotec Inc., Auburn, CA).

Techniques: Significance Assay

Journal: Molecular Vision

Article Title: P2X 7 receptor activation may be involved in neuronal loss in the retinal ganglion cell layer after acute elevation of intraocular pressure in rats

doi:

Figure Lengend Snippet: Representative photomicrographs of double immunostaining of the P2X 7 receptor and CD68 in the normal retina and in the retina on days 1, 2, 3, and 7 after intraocular pressure elevation. P2X 7 -positive cells were also stained with anti-CD68 in the ganglion cell layer (GCL). Bar=100 µm.

Article Snippet: For this, mouse Alexa Fluor 488–labeled anti-TUJ1 monoclonal antibody (A488–435L, Covance Research Products, Princeton, NJ) and mouse anti-CD68 (MAB1435, Merck Millipore, Billerica, MA) were used.

Techniques: Double Immunostaining, Staining

Journal: Molecular Vision

Article Title: P2X 7 receptor activation may be involved in neuronal loss in the retinal ganglion cell layer after acute elevation of intraocular pressure in rats

doi:

Figure Lengend Snippet: Representative photomicrographs of double immunostaining of CD68 and TNF-α or interleukin-1β (IL-1 β) in the normal retina and the retina on day 2, with and without treatment of oxidized adenosine triphosphate (OxATP) at 30 µM just after intraocular pressure (IOP) elevation. A: TNF- α, B: IL-1 β. Immunoreactivities of tumor necrosis factor-α (TNF-α) and IL-1β were upregulated in the ganglion cell layer (GCL) and inner plexiform layer (IPL) cells on day 2 after IOP elevation; they were subsequently suppressed by treatment with OxATP. Bar=100 µm.

Article Snippet: For this, mouse Alexa Fluor 488–labeled anti-TUJ1 monoclonal antibody (A488–435L, Covance Research Products, Princeton, NJ) and mouse anti-CD68 (MAB1435, Merck Millipore, Billerica, MA) were used.

Techniques: Double Immunostaining