Journal: Journal of Neuroscience

Article Title: Suppression of Alzheimer-Associated Inflammation by Microglial Prostaglandin-E2 EP4 Receptor Signaling

doi: 10.1523/jneurosci.0410-14.2014

Figure Lengend Snippet: Figure 5. EP4 receptor conditional knock-out in microglia enhances inflammation and amyloid deposition early at 5 months in APPSwe-PS1E9 (APPS) mice. Microglial EP4 receptor conditional knock-out (EP4-cKO) 5 month female and 9 month male APPS or nontransgenic (Non-tg) mice were generated using the CD11b-Cre; EP4 lox/lox strategy. A, qRT-PCR from hippocampal RNA demonstrates elevated CCL3 expression in APPS EP4-cKO mice at 5 months but not 9 months, and a trend toward increased IL-1 in 5 month APPS-EP4cKO mice. B, LC/ESI/MS/MS analysis of cortical lysates demonstrates elevated -ketoaldehyde adduct levels in young APPS EP4-cKO mice at 5 months. A40 (C) and (D) A42 ELISAs from guanidine-extracted cerebral cortex demonstrate increased A levels in EP4-cKO mice in 5 month but not 9 month APPS-EP4cKO mice. E, Immunostaining for 6E10-positive A plaques (arrows) and quantification of A plaque area in the hippocampus (F) demonstrates increased A plaque area and density in APPS EP4-cKO mice at 5 months of age. G, Congo Red staining for plaque cores (white arrows) and quantification of Congo Red-positive area in the hippocampus and cerebral cortex (H) demonstrates increased dense-core plaque percentage area in APPS EP4-cKO mice (A–H; n 4–11 mice per group). *p 0.05 (unpaired t test). **p 0.01 (unpaired t test). ***p 0.0001 (unpaired t test). I, J, Quantitative Western analysis of PSD-95 demonstrates significant decrease in 5 month (n 4/genotype; p 0.05) and in 9 month cohorts (n 3–5 mice per genotype; ANOVA p 0.032, post hoc Bonferroni multiple comparison. *p 0.05, APP-PS1;Cd11bCre; EP4 / versus APP-PS1;Cd11Cre;EP4 lox/lox, with conditional deletion of EP4 in APP-PS1 mice.

Article Snippet: Immunocytochemistry for mouse EP4 was performed using a chicken antibody directed against the mouse EP4 receptor (1:500), described and validated in (Liang et al., 2011), and a rat monoclonal antibody directed against mouse CD11b (1:500, AbD Serotec).

Techniques: Knock-Out, Generated, Quantitative RT-PCR, Expressing, Tandem Mass Spectroscopy, Immunostaining, Staining, Western Blot, Comparison

Journal: BMC Molecular and Cell Biology

Article Title: Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages

doi: 10.1186/s12860-020-00331-9

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: CD11b/c Biotin (OX-42) , CD11b/c, CR3 , Cedarlane , CL042B , 2 μg/ml.

Techniques: Concentration Assay, Flow Cytometry, Control, Staining, FACS