Journal: bioRxiv

Article Title: LSD1 inhibitors induce neuronal differentiation of Merkel cell carcinoma by disrupting the LSD1-CoREST complex and activating TGFβ signaling

doi: 10.1101/2020.04.14.041657

Figure Lengend Snippet: a , Bulk mouse skin labeled with IgM-PE mouse isotype control. b , Bulk mouse skin labeled with NCAM-PE. DAPI - cells were sorted. c , Merkel cells labeled with IgM-PE mouse isotype control. d , Merkel cells labeled with NCAM-PE. DAPI - /NCAM + cells were sorted. Each plot shows the subpopulation gated for in the preceding plot to the left.

Article Snippet: Merkel cells were stained with anti-PSA-NCAM-PE antibody (Miltenyi Biotec, Cat. 130-117-394) or with mouse IgM-PE isotype control (Miltenyi Biotec, Cat. 130-120-070) according to manufacturer’s instructions, for 10 min at 4°C.

Techniques: Labeling, Control

Journal:

Article Title: Protein kinase C activation inhibits eosinophil degranulation through stimulation of intracellular cAMP production

doi: 10.1038/sj.bjp.0706028

Figure Lengend Snippet: Western blot analysis of the expression of PKC isoforms in human peripheral blood eosinophils from a single patient, representative of six others. Rat brain extract was run as positive control and blotted with nonspecific PKC antibody. Exposure times for the ELC reaction were ⩽1 min for PKCs δ and ζ and 60 min for all other isoforms.

Article Snippet: The cAMP assay kit (direct method) was obtained from Assay Designs Inc, Ann Arbor, Michigan, U.S.A. All the anti-PKC antibodies, the HRP-labelled secondary antibodies and rat brain extract were obtained from Santa Cruz Biotechnology, Santa Cruz, CA, U.S.A.

Techniques: Western Blot, Expressing, Positive Control