ras Search Results


anti ras  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti ras
Anti Ras, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti k ras  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti k ras
Anti K Ras, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rab35  (Proteintech)
93
Proteintech rab35
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Rab35, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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plex uorf ha bira n ras q61k t35a ires puro  (Addgene inc)
91
Addgene inc plex uorf ha bira n ras q61k t35a ires puro
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Plex Uorf Ha Bira N Ras Q61k T35a Ires Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pan ras  (Proteintech)
94
Proteintech pan ras
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Pan Ras, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal rabbit anti ras  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc monoclonal rabbit anti ras
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Monoclonal Rabbit Anti Ras, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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anti panras c 4  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti panras c 4
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Anti Panras C 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gtp rheb antibody  (Proteintech)
93
Proteintech gtp rheb antibody
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Gtp Rheb Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rab7  (Proteintech)
96
Proteintech rab7
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Rab7, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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18295 1 ap  (Proteintech)
95
Proteintech 18295 1 ap
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
18295 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rasd1 a05991 1  (Boster Bio)
93
Boster Bio rasd1 a05991 1
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Rasd1 A05991 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human rasef  (Proteintech)
85
Proteintech human rasef
Fig. 5. FIP1 is required for maintaining <t>Rab35</t> at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.
Human Rasef, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 5. FIP1 is required for maintaining Rab35 at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.

Journal: Journal of cell science

Article Title: Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

doi: 10.1242/jcs.244384

Figure Lengend Snippet: Fig. 5. FIP1 is required for maintaining Rab35 at the midbody. (A) Top: representative images of cells progressing through mitosis that stably express either GFP-FIP1B full length (green) or mCherry-Rab35 (yellow). Cells are stained with SiR-tubulin and tubulin signal is shown in magenta. Time zero is set as the frame corresponding to telophase. Arrowheads denote the ICB linking daughter cells or the remnants of the spindle after abscission. Bottom left: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. Bottom right: normalized average fluorescence intensity of either GFP-FIP1B (green) or mCherry-Rab35 (red) over time at the midbody, where time point 0 is the first observance of telophase. mCherry-Rab35, n=10; GFP-FIP1B, n=18. (B) Representative western blot showing the depletion of FIP1 or Rab35 in the subsequent experiment. FIP1 antibody has non-specific bands and a single asterisk denotes FIP1B, and a double asterisk denotes FIP1C. (C) Top: representative images of cells progressing through mitosis that stably express mCherry-Rab35 (shown in yellow) that were treated with either siNT or siFIP1. Arrowheads denote the location of abscission. Bottom left: normalized average fluorescence intensity of mCherry-Rab35 over time, following either siNT or siFIP1 treatment, at the cell-cell interface between daughter cells, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Bottom right: normalized average fluorescence intensity of mCherry- Rab35 over time, following either siNT or siFIP1 treatment, at the midbody, where time point 0 is the first observance of telophase. siNT, n=10; siFIP1, n=8. Data in A and C are presented as mean±s.e.m. *P<0.05; ***P<0.001; ****P<0.0001 (unpaired two-tailed Student’s t-test). a.u., arbitrary units.

Article Snippet: Antibodies and other staining reagents Primary antibodies used were as follows: Rab11FIP1 (Sigma-Aldrich, HPA025960, used at 1/1000); Rab11FIP2 (Sigma-Aldrich, HPA037726, used at 1/1000); Rab11FIP5 (Sigma-Aldrich, HPA036407, used at 1/1000); Rab11FIP3 (Proteintech, 25843-1-AP, used at 1/100 for immunofluorescence); actin [Millipore (C4), MAB1501, used at 1/10,000]; GFP [Santa Cruz Biotechnology (B-2), sc-9996, used at 1/2000]; GAPDH [Santa Cruz Biotechnology (FL-335), sc-25778, used at 1/1000]; mCherry for immunoprecipitation (Abcam, ab183628, 1 μg used per immunoprecipitation condition); mCherry for western blotting (Abcam, ab167453, used at 1/2000); MICAL1 (Proteintech, 14818-1-AP, used at 1/100 for immunofluorescence); Rab35 (Proteintech, 11329-2-AP, used at 1/1000); MKLP1 (Abcam, ab168964, used at 1/100 for immunofluorescence); CHMP4B (Proteintech, 13683-1-AP, used at 1/100 for immunofluorescence); His-probe [Santa Cruz Biotechnology (H-15), sc-803, used at 1/1000]; β-Tubulin (Abcam, ab6046, used at 1/2000); and α-Tubulin [Cell Signaling Technology (DM1A), 3873, used at 1/1000 for immunofluorescence].

Techniques: Stable Transfection, Staining, Fluorescence, Western Blot, Two Tailed Test