Journal: American Journal of Cancer Research

Article Title: NRSN2 promotes osteosarcoma cell proliferation and growth through PI3K/Akt/MTOR and Wnt/β-catenin signaling

doi:

Figure Lengend Snippet: NRSN2 regulates PI3K/Akt/GSK3β axis and Wnt/β-catenin signaling in osteosarcoma cells. A. The level of phosphorylated Akt, mTOR, p-GSK3β and nuclear β-catenin (nu-β-catenin) are positively correlated with the level of NRSN2 in U2OS and MG63 cells. B. Luciferase reporter assays revealed that NRSN2 could regulate Wnt/β-catenin signaling in U2OS and MG63 cells. C. Knockdown NRSN2 inhibits the expression of CCND1 and c-myc in U2OS cells. D. Overexpression of NRSN2 elevates the mRNA levels of CCND1 and c-myc in MG63 cells. E. The pro-proliferation effect was reversed when treated with IWR-1-endo, a inhibitor of β-catenin. *p<0.05, **p<0.01.

Article Snippet: The following antibodies were used in this study: NRSN2 (1:1000, Proteintech), GAPDH (1:5000, Proteintech), p-Akt, Akt, p-mTOR, mTOR, p-GSK3β, GSK3β, β-catenin and the second antibodies were purchased from Cell Signaling Technology and with 1:1000 dilutions.

Techniques: Luciferase, Expressing, Over Expression

Journal: Neuropsychiatric Disease and Treatment

Article Title: Shisandra Decoction Alleviates Parkinson’s Disease Symptoms in a Mouse Model Through PI3K/AKT/mTOR Signalling Pathway

doi: 10.2147/NDT.S476969

Figure Lengend Snippet: AKT and mTOR phosphorylation and relative abundance of p70S6K. ( A ) WB electrophoretogram. ( B ) and ( C ) Relative degree of AKT and mTOR phosphorylation. ( D ) Relative abundance of p70S6K. Use the Tukey HSD test statistical method. *P < 0.05, **P < 0.01 compared to control. # P < 0.05, ## P < 0.01 compared to model group. Δ P < 0.05, ΔΔ P < 0.01 compared to LY294002 group.

Article Snippet: MPTP (Shanghai Yuanye Biotechnology Co., Ltd., item number S31504-500mg); LY294002 (MedChemExpress, item number HY-10108); LC3 antibody, AKT antibody, p-AKT antibody, PTEN antibody, PI3K antibody, mTOR antibody, p-mTOR antibody, p70S6K antibody, p62/SQSTM1 antibody, α-syn antibody (Wuhan Biode Biotechnology Engineering Co., Ltd., item numbers: BM4827, BM1612, BM4762, A00006-1, PB0351, BM4182, BM4840, M01475-4, PB0458, M00215-3, respectively).

Techniques: Phospho-proteomics, Control

Journal: Cell Death & Disease

Article Title: Microglia as modulators of exosomal alpha-synuclein transmission

doi: 10.1038/s41419-019-1404-9

Figure Lengend Snippet: Whole-cell lysates were analyzed by western blot. a Protein levels of LC3 ( n = 5, ** p = 0.0082), P62/SQSTM1 ( n = 5, **** p < 0.0001), Beclin ( n = 5, * p = 0.0101), phospho-AKT ( n = 5, *** p = 0.0004), total-AKT, phospho-mTOR ( n = 4, * p = 0.0183), and β-actin were shown in indicated cells. b Whole-cell lysates were immunoblotted against α-syn (oligomeric) ( n = 5, ** p = 0.0078); monomeric ( n = 4, * p = 0.0113) and β-actin for control. c The triple-stained image of P62/SQSTM1 (gray) with IBA1 (green) and DAPI (blue) in the SNpc

Article Snippet: The following primary antibodies were utilized: rabbit antibody to IBA1 (1:1000, 10904-1-AP, Proteintech), mouse antibody to tsg 101 (1:500, sc-7964, Santa cruz biotechnology), mouse antibody to CD63 (1:1000, ab59479, Abcam), rabbit antibody to Calnexin (1:1000, 10427-2-AP, Proteintech), rabbit antibody to α-syn (1:1000, ab138501, ab52168, Abcam), rabbit antibody to Oligomer (1:1000, AHB0052, ThermoFisher scientific), rabbit antibody to TH (1:1000, 25859-1-AP, Proteintech), rabbit antibody to LC3 (1:1000, 14600-1-AP, Proteintech), rabbit antibody to SQSTM1/P62 (1:1000, ab91526, Abcam), rabbit antibody to Beclin1 (1:1000, 11306-1-AP, Proteintech), rabbit antibody to phosphor-AKT (1:800, AP0655, Abclonal), rabbit antibody to AKT (1:800, A11030, Abclonal), rabbit antibody to phosphor-mTOR (1:400, BM4840, Boster), mouse antibody to α-syn (1:1000, synuclein Ab-2, Thermo Scientific), and rabbit antibody to p-syn (1:500, GTX50222, Genetex).

Techniques: Western Blot, Control, Staining

Journal: bioRxiv

Article Title: Argon inhibits autophagy and improves cerebral ischemia-reperfusion injury via PI3K/Akt/mTOR pathway

doi: 10.1101/2025.10.31.685964

Figure Lengend Snippet: A: western blot results showed that argon treatment significantly upregulated the levels of p-PI3K, p-Akt, and p-mTOR. B: Bar chart for A; C: western blot results of PI3K/Akt/mTOR pathway factors and autophagy factors in N 2, N 2 combined with SC79, and N 2 combined with MHY1485 group; D: Bar chart for C. E: western blot results of in argon, argon combined with MK2206 and argon combined with rapamycin groups. F: Bar chart for E. MHY: MHY1485; Rapa: rapamycin.

Article Snippet: The first antibodies included: P62 (Abcam, 1:1000, rabbit derived polyclonal antibody, ab91526), LC3 (Sigma Aldrich, 1:500, rabbit derived polyclonal antibody, ABC929), β-actin (MCE, 1:5000, mouse monoclonal antibody, HY-P80438), PI3K (Abcam, 1:1000, rabbit derived monoclonal antibody, ab302958), p-PI3K (Abcam, 1:1000, rabbit derived polyclonal antibody, ab138364), Akt (MCE, 1:2000, rabbit monoclonal antibody), p-Akt (MCE, 1:1000, rabbit monoclonal antibody, HY-P80276), mTOR (MCE, 1:1000, rabbit polyclonal antibody, HY-P80276), p-mTOR (MCE, 1:1000, rabbit monoclonal antibody, HY-P80837).

Techniques: Western Blot