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List of antibodies used in this study
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List of antibodies used in this study
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List of antibodies used in this study
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Phenotype of the type II cells cultured on collagen-coated <t>wells.</t> <t>Epithelial</t> cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products <t>(RAGE).</t> A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.
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Image Search Results


List of antibodies used in this study

Journal: Acta neuropathologica

Article Title: Beta-Amyloid pathology in human brain microvessel extracts from the parietal cortex: Relation with cerebral amyloid angiopathy and Alzheimer’s Disease

doi: 10.1007/s00401-019-01967-4

Figure Lengend Snippet: List of antibodies used in this study

Article Snippet: Protein Role/localization Host Dilution Company Catalog number WB IF α-SMA Smooth muscle cell Rabbit 1:1000 1:100 Abcam ab5694 Aβ40 Aβ peptide Mouse ---------- 1:100 BioLegend 805401 Aβ42 Aβ peptide Mouse ---------- 1:100 BioLegend 805501 ABCB1 BBB transporter Rabbit 1:2000 1:100 Abcam ab170904 APP Aβ precursor Mouse 1:500 ---------- BioLegend 803003 BACE1 Aβ production Rabbit 1:500 ---------- Abcam ab108394 CD31 EC intercellular junctions Rabbit 1:1000 ---------- Abcam ab28364 Claudin-5 EC intercellular junctions Rabbit 1:2000 1:100 Santa Cruz Biotechnology sc-28670; discontinued Cyclophilin B Loading control Rabbit 1:1000 ---------- Abcam ab16045 LRP1 BBB receptor Rabbit 1:20000 ---------- Abcam ab92544 Neprilysin Aβ-degrading enzyme Rabbit 1:2000 ---------- Abcam ab79423 NeuN Neuronal nuclei Rabbit 1:1000 1:1000 Abcam ab177487 Occludin EC intercellular junctions Rabbit 1:1000 ---------- Invitrogen 71-1500 PDGFRβ Pericyte receptor Rabbit 1:2000 1:100 Abcam ab32570 RAGE BBB receptor Rat 1:2000 ---------- R&D Systems MAB11795 Synaptophysin Presynaptic vesicles Mouse 1:20000 ---------- Millipore MAB368; discontinued Type IV collagen Basal membrane Goat ---------- 1:500 Millipore AB769 Open in a separate window Abbreviations: Aβ, beta amyloid peptides; ABCB1, P-glycoprotein; APP, amyloid protein precursor; BACE1, β-secretase; BBB, blood-brain barrier; CD31, platelet endothelial cell adhesion molecule; EC, endothelial cell; IF, immunofluorescence; LRP1, low density lipoprotein receptor-related protein 1; NeuN, neuronal nuclei; RAGE, receptor for advanced glycation end-products; WB, Western blot.

Techniques: Control, Membrane

Phenotype of the type II cells cultured on collagen-coated wells. Epithelial cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products (RAGE). A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Transitional human alveolar type II epithelial cells suppress extracellular matrix and growth factor gene expression in lung fibroblasts

doi: 10.1152/ajplung.00337.2018

Figure Lengend Snippet: Phenotype of the type II cells cultured on collagen-coated wells. Epithelial cells were cultured alone or with fibroblasts on collagen-coated coverslips or tissue culture wells. After 2 days for adherence the cells were cultured with 5% FBS, as in Fig. 6. By immunocytochemistry, the epithelial cells expressed both Muc1 and receptor for advanced glycation end-products (RAGE). A: blue DAPI. B: red Muc1. C: green RAGE. D: combined.

Article Snippet: The primary antibodies were epithelial membrane protein 2 (EMP2) (HPAA014711; Sigma-Aldrich, St. Louis, MO), RAGE (AF1145; R&D Systems) smooth muscle actin (A2547; Sigma-Aldrich), and GAPDH (ab8245; Abcam, Cambridge, MA).

Techniques: Cell Culture, Immunocytochemistry