Journal: Nature Communications

Article Title: Targeting modulation of the choroid plexus blood-CSF barrier and CSF hypersecretion via lipid nanoparticle-mediated co-delivery of siRNA and resveratrol

doi: 10.1038/s41467-025-61543-1

Figure Lengend Snippet: a Schematic illustration of the functions of siR/RSV@TNP in ChP epithelial cells. Illustration created using Microsoft PowerPoint, incorporating elements (siR/RSV@TNP and SPAK siRNA) adapted from in BioRender. Gao, H. (2025) https://BioRender.com/i60a962 . b The mRNA expression of SPAK after different treatments, n = 4 biological replicates for each group. c Western blot result of SPAK. d Immunofluorescence of SPAK. Scale bar, 20 μm. e Western blot result of SPAK. f , g The mRNA expression of NKCC1 and KCNJ13 after different treatments, n = 4 biological replicates for each group respectively. h Western blot result of p-SPAK, n = 3 biological replicates for each group. i Immunofluorescence of pSPAK. Scale bar, 20 μm. All data are presented as mean ± SD. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test or Dunnett’s multiple comparisons test were used for the statistical comparison among multiple groups, respectively. Statistical analyses were conducted with a 95% confidence interval, and significance was defined as p < 0.05. Source data are provided as a Source Data file.

Article Snippet: The expression of SPAK an NKCC1 protein was detected using the Western Blotting analysis, employing specific antibodies for SPAK (ABclonal, A2275, 1:1000) and NKCC1 (Proteintech, 13884-1-AP, 1:1000).

Techniques: Expressing, Western Blot, Immunofluorescence, Comparison