Journal: Frontiers in Neuroscience

Article Title: Additive Effects of Environmental Enrichment and Ketamine on Neuropathic Pain Relief by Reducing Glutamatergic Activation in Spinal Cord Injury in Rats

doi: 10.3389/fnins.2021.635187

Figure Lengend Snippet: Primary antibodies for western blotting.

Article Snippet: Rabbit anti-phospho-NMDAR2B Tyr 1472 (p-NR2B) , 1:1,000 , Merck Millipore, Darmstadt, Germany.

Techniques: Western Blot

Journal: The Journal of Neuroscience

Article Title: Pharmacological Rescue of Long-Term Potentiation in Alzheimer Diseased Synapses

doi: 10.1523/JNEUROSCI.2774-16.2016

Figure Lengend Snippet: Figure 1.

Article Snippet: Antibodies were from Cell Signaling Technology: ERK1/2 (catalog #9102 RRID:AB_330744), GluA1 (13185); from Millipore: GluA1 (ABN241), GluA2-AlexaFluor-488 (catalog #MAB397A4, RRID:AB_10917113), PSD95 (mouse, catalog #MAB1598 RRID:AB_94278), TrkB (catalog #07–225 RRID:AB_310445), Synapsin-I (catalog #AB1543 RRID:AB_2200400), Synaptophysin (catalog #MAB5258 RRID:AB_2313839); from University of California-Davis/National Institutes of Health NeuroMab Facility: Neurexin-1β (catalog #75–216, RRID:AB_2155531), VGluT1 (catalog #75–066 RRID:AB_2187693); from Abcam: histone-H3 (trimethyl K4, catalog #ab8580 RRID:AB_306649), PSD95 (rabbit, catalog #ab18258 RRID:AB_444362); from Invitrogen: Secondary antibodies, AlexaFluor-488 (anti-rabbit, catalog #A-11034 RRID:AB_2576217), AlexaFluor-488 (anti-mouse, catalog #A-11029 RRID:AB_2534088), AlexaFluor-647 (anti-rabbit, catalog #A-21245 RRID:AB_2535813), AlexaFluor-647 (anti-mouse, catalog #A-21236 RRID:AB_2535805); from Sigma-Aldrich: β-actin (catalog #A2066 RRID:AB_476693); from GeneTex: GAPDH (catalog #GTX100118 RRID:AB_1080976), p84 (catalog #GTX70220 RRID:AB_372637); from Pierce: Secondary HRP (anti-mouse, catalog #31438 RRID:AB_228217), secondary HRP (anti-rabbit, catalog #31460 RRID:AB_228341); from Alomone Labs: GluN2B (catalog #AGC-003 RRID:AB_2040028); and calcein acetoxymethyl ester (calcein AM; ultrapure grade, Affymetrix, eBioscience, #65-0853-39).

Techniques:

Journal: Diving and Hyperbaric Medicine

Article Title: Inhibition of NR2B-containing NMDA receptors during nitrogen narcosis

doi: 10.28920/dhm49.4.276-282

Figure Lengend Snippet: The increases in the phosphorylated levels of NR2B and CREB in cultured hippocampal neurons after NMDA incubation were inhibited by 1.6 MPa N2-O2 exposure. (A) Representative expression of p-GluA1 measured using western blot analysis. (B) Fold changes in the gray intensity of p-GluA1/GluA1 and GluA1/GAPDH, n = 3 in each group, the data were analyzed using the Mann-Whitney U test. (C) Representative expression of p-NR2B measured using western blot analysis. (D) Fold changes in the gray intensity of p-NR2B/NR2B, n = 3 in each group, one-way analysis of variance and LSD for post hoc test were used. (E) Immunofluorescent images showing the pCREB expression (green in a and d) in the nucleus (blue, DAPI staining b and e), scale bar 25 µm, n = 4 in each group

Article Snippet: Polyvinylidene fluoride membranes with transferred proteins were blocked with 5% milk in tris buffered saline plus 0.1% Tween-20 for one hour and then with the following primary antibodies overnight at 4°C: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Millipore, Darmstadt, Germany); rabbit anti-cleaved caspase-3 polyclonal antibody 1:1000 (Cell Signaling Technology (CST), Danvers, USA); mouse anti-β-actin 1:8000 (Sigma-Aldrich Co., St. Louis, USA); rabbit anti-AMPA Receptor 1 (GluA1) (CST, Danvers, USA); and rabbit anti-Phospho-AGluA1 (Ser845) (CST, Danvers, USA), phospho-NR2B (CST, Danvers, USA), and total NR2B (CST, Danvers, USA).

Techniques: Cell Culture, Incubation, Expressing, Western Blot, MANN-WHITNEY, Staining