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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model
doi: 10.1016/j.jbc.2021.100482
Figure Lengend Snippet: Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.
Article Snippet: Chem. (2021) 296 100482 After permeabilization and blocking, sections were incubated with primary antibodies (anti-insulin (Cat #66198-1-Ig, Proteintech Group, Inc); anti-glucagon (Cat # 15954-1- AP,Proteintech Group, Inc)) overnight at 4 C and incubated with appropriate
Techniques: Generated, Microscopy, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation
Journal:
Article Title: Angiotensin II plays a pathogenic role in immune-mediated renal injury in mice
doi:
Figure Lengend Snippet: Immunohistochemical findings in the kidney after the administration of anti-GBM AS in the AT1a+/+ (a, c, and e) and AT1a–/– mice (b, d, and f). Linear binding of rabbit IgG to the GBM at 6 h (a and b). Fine granular deposits of C3 along the capillary wall, with additional deposition in the mesangium at 6 h (c and d). Linear binding of mouse IgG, identical to rabbit IgG, in the glomeruli at 7 days (e and f). ×400.
Article Snippet: The immunostaining of
Techniques: Immunohistochemical staining, Binding Assay