Journal: Scientific Reports

Article Title: Increased inflammation, oxidative stress and mitochondrial respiration in brown adipose tissue from obese mice

doi: 10.1038/s41598-017-16463-6

Figure Lengend Snippet: Increased immune cell infiltration in BAT from obese mice. Histological sections from interscapular BAT, eWAT and iWAT were analysed. ( A ) Representative images of Masson´s trichrome staining (left). Total collagen was quantified and expressed as the percentage of stained area in blue (right). ( B ) Representative images of immunohistochemical localization of Caspase-3 (left). The stained area was quantified and apoptosis is expressed as the number of dead cells (right). ( C ) Representative images of immunohistochemical localization of Mac-2 (left). The stained area was quantified and macrophage infiltration was expressed as a percentage of the stained area (right). ( D ) Representative images of immunohistochemical localization of CD3 (left). T cell infiltration was expressed through the quantification of CD3 positive cells (right). Arrows point to stained areas. Results are represented as mean ± SEM. n = 8–10; *p < 0.05; **p < 0.01.

Article Snippet: For T cells staining, we used primary polyclonal rabbit anti-murine CD3 antibody (1/4,000; Cell Marque).

Techniques: Staining, Immunohistochemical staining