Journal: Stem Cells International

Article Title: Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells

doi: 10.1155/2016/8582526

Figure Lengend Snippet: Immunohistochemistry of hESCs, haGSCs, and hFibs with CD9, CD24, Oct4, and Nanog antibodies. The different markers are shown in green and the staining of the nuclei with DAPI in blue. Scale bar is 100 μ m.

Article Snippet: The following primary antibodies were used as stem cell markers: mouse monoclonal anti-CD9 (R&D System, Stem cell marker kit, SC009), mouse monoclonal anti-CD24 (Abcam, ab31622), rabbit polyclonal anti-OCT4 (Abcam, ab19857), and rabbit polyclonal anti-nanog (Abcam, ab21624).

Techniques: Immunohistochemistry, Staining

Journal: eLife

Article Title: A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx

doi: 10.7554/eLife.72849

Figure Lengend Snippet: ( A, B ) Representative flow cytometry plots and proportions ( A ) and quantitative summary ( B ) of neutrophils in peripheral blood are shown. Anti-Ly6G antibody or rat IgG2a isotype antibody were intraperitoneally injected into C57BL/6 mice (18–20 g, n = 6) at doses of 500 µg/mouse on day 0 and day 2, respectively. At day 3, peripheral blood was collected for testing the scavenging efficiency of neutrophils by flow cytometry. ( C, D ) Representative flow cytometry plots and proportions ( C ) and quantitative summary ( D ) of neutrophils in peritoneal lavage are shown. Anti-CSF1R antibody or rat IgG2a isotype antibody were intraperitoneally injected into C57BL/6 mice (18–20 g, n = 6) at doses of 1 mg/mouse on day 0 followed by 0.3 mg/mouse on day 1 and day 2, respectively. At day 3, peritoneal lavage was collected for testing the scavenging efficiency of monocytes/macrophages by flow cytometry. *** p < 0.001, ns, not significant.

Article Snippet: Antibody , Rat monoclonal anti-IgG2a , BioXcell , Clone: 2A3, Cat#: BE0089, RRID: AB_1107769 , Isotype control for anti-mouse Ly6G and anti-mouse CSF1R.

Techniques: Flow Cytometry, Injection

Journal: eLife

Article Title: A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx

doi: 10.7554/eLife.72849

Figure Lengend Snippet: ( A, B ) Protective efficacy of Popu CATH against E. coli ( A ) or S. aureus ( B ) in neutrophil depletion mice. Anti-Ly6G antibody or rat IgG2a isotype antibody were intraperitoneally injected into C57BL/6 mice (18–20 g, n = 6) at doses of 500 µg/mouse on day 0 and day 2, respectively. ( C, D ) Protective efficacy of Popu CATH against E. coli ( C ) or S. aureus ( D ) in monocyte/macrophage depletion mice. Anti-CSF1R antibody or rat IgG2a isotype antibody were intraperitoneally injected into C57BL/6 mice (18–20 g, n = 6) at doses of 1 mg/mouse on day 0 followed by 0.3 mg/mouse on day 1 and day 2, respectively. ( E, F ) Protective efficacy of Popu CATH against E. coli ( E ) or S. aureus ( F ) in Rag1 –/– mice (18–20 g, n = 6). At 4 hr before E. coli or S. aureus (2 × 10 7 CFUs/mouse) inoculation, Popu CATH (10 mg/kg) was intraperitoneally injected into neutrophil depletion mice (on day 3), monocyte/macrophage depletion mice (on day 3), and Rag1 –/– mice. At 18 hr post bacterial inoculation, peritoneal lavage was collected for the bacterial load assay. ***p < 0.001, ns, not significant.

Article Snippet: Antibody , Rat monoclonal anti-IgG2a , BioXcell , Clone: 2A3, Cat#: BE0089, RRID: AB_1107769 , Isotype control for anti-mouse Ly6G and anti-mouse CSF1R.

Techniques: Injection

Journal: eLife

Article Title: A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx

doi: 10.7554/eLife.72849

Figure Lengend Snippet:

Article Snippet: Antibody , Rat monoclonal anti-IgG2a , BioXcell , Clone: 2A3, Cat#: BE0089, RRID: AB_1107769 , Isotype control for anti-mouse Ly6G and anti-mouse CSF1R.

Techniques: Flow Cytometry, cDNA Library Assay, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Staining, Blocking Assay, In Vivo, Control

Journal:

Article Title: Mucosal Vaccination with Recombinantly Attenuated Staphylococcal Enterotoxin B and Protection in a Murine Model

doi: 10.1128/IAI.69.4.2031-2036.2001

Figure Lengend Snippet: Isotyping of SEB-specific antibodies in pooled sera after three vaccinations of SEBv given nasally, orally, or i.p. Mice were given 20 μg of SEBv and 5 or 10 μg of CT (nasal and oral routes, respectively) or alum (i.p.) per dose. Controls received adjuvant without SEBv by the same inoculation routes. Sera were pooled within each group (n = 10 mice) and diluted 1:100. Data represent the mean absorbance of duplicate wells ± 10%.

Article Snippet: SEB-specific antibodies were isotyped by an ELISA with a panel of rabbit anti-mouse immunoglobulin sera (Bio-Rad Laboratories, Hercules, Calif.) as described by the manufacturer.

Techniques:

Journal:

Article Title: Mucosal Vaccination with Recombinantly Attenuated Staphylococcal Enterotoxin B and Protection in a Murine Model

doi: 10.1128/IAI.69.4.2031-2036.2001

Figure Lengend Snippet: Comparison of serum anti-SEB IgG titers among nasally and orally vaccinated mice after two (A) and three (B) inoculations with 2.5 or 20 μg of SEBv plus CT. Pooled sera from commonly immunized mice (n = 10 per group) and control animals (CT oral shown, although CT nasal and alum i.p.-only controls also resulted in similar data) were diluted serially (1:50 to 1:156,250). Data represent the mean absorbance of triplicate wells ± standard deviation.

Article Snippet: SEB-specific antibodies were isotyped by an ELISA with a panel of rabbit anti-mouse immunoglobulin sera (Bio-Rad Laboratories, Hercules, Calif.) as described by the manufacturer.

Techniques: Standard Deviation

Journal: Cell

Article Title: Macrophages direct location-dependent recall of B cell memory to vaccination.

doi: 10.1016/j.cell.2025.04.005

Figure Lengend Snippet: Figure 4. SSMs in the dLN subcapsular niche control Bmem cell fate (A) Schematic for comparing swapping the source of T cell help in Bmem recall response. (B) Representative flow cytometric analysis of boosted dLN (left) and ndLN (right) showing effect of swapping T cell help from OT-II mTfh to Smarta naive T cells. (C) Enumeration of donor-derived antigen-specific total B cells, GC B cells, and PCs in boosted dLN and ndLN in recall response to HEL-OVA and HEL-gp61 boost. Data were pooled from three in- dependent experiments. (D) Schematic for determining the impact of SSM depletion on Bmem recall responses in dLN vs. ndLN. (E) Representative plots showing the effect of isotype control (left) or anti-CSF1R mAb (right) on GC B cell and PC differentiation in boosted dLN (top) and boosted ndLN (bottom). (F) Enumeration of donor-derived antigen-specific total B cells, GC B cells, and PCs in boosted dLN and ndLN treated with isotype control or anti-CSF1R. Data were pooled from two independent experiments. In (C) and (F), each circle or square represents one mouse. Line represents median.

Article Snippet: In vivo monoclonal antibody treatment Anti-mouse CSF1R mAb (clone AFS98, Bio X Cell) was used to deplete SSMs.

Techniques: Control, Derivative Assay

Journal: Cell

Article Title: Macrophages direct location-dependent recall of B cell memory to vaccination.

doi: 10.1016/j.cell.2025.04.005

Figure Lengend Snippet: Figure 6. Primed SSMs mediate superior Bmem responses agnostic of the antigen (A) Schematic for synchronous priming of the contralateral ndLN with the irrelevant antigen R-phycoerythrin (PE). (B) Enumeration of total number of donor-derived antigen-specific B cells, GC B cells, and PCs in contralaterally HEL-OVA-boosted ndLN treated with isotype control or anti-CSF1R mAb. (C) Percentage of GC B cells and PCs within donor-derived antigen-specific B cells in contralaterally HEL-OVA-boosted ndLN treated with isotype control or anti- CSF1R mAb. Data were pooled from two independent experiments. Each circle represents one mouse. Line and bar represent median.

Article Snippet: In vivo monoclonal antibody treatment Anti-mouse CSF1R mAb (clone AFS98, Bio X Cell) was used to deplete SSMs.

Techniques: Derivative Assay, Control