Journal:

Article Title: Bak and Bax Function To Limit Adenovirus Replication through Apoptosis Induction

doi: 10.1128/JVI.76.9.4547-4558.2002

Figure Lengend Snippet: Conformational changes in Bak and Bax and Bak-Bax interaction in Ad5dl337- and Ad5E1B−-infected HeLa cells. (A) Immunoprecipitation (IP) of Bak from mock-, Ad5dl309-, Ad5dl337-, and Ad5E1B−-infected cells. Immunoprecipitations were carried out with anti-BRCA-2, anti-Bak(23-37), anti-Bak(Ab-1), and anti-Bak(−TM) antibodies (Abs) from the soluble fraction of cells lysed in CHAPS-containing buffer at 24 and 48 h postinfection. Ad5dl309-infected HeLa cell lysate (M) was used as a marker for Bak, Bax, and E1B 19K. Western blotting was carried out on precipitated material with a combination of anti-Bak and anti-Bax antibodies (top four panels) or with anti-E1B 19K antibody (bottom four panels). (B) Immunoprecipitation of Bax from mock-, Ad5dl309-, Ad5dl337-, and Ad5E1B−-infected cells. Experiment was executed as for panel A except that immunoprecipitation was carried out with anti-Myc, anti-Bax(11-30), anti-Bax(150-165), and anti-Bax(43-61) antibodies. Western blotting was carried out as for panel A.

Article Snippet: Immunoprecipitations in Fig. were carried out with the following antibodies: anti-Myc rabbit polyclonal (Santa Cruz); anti-Bax(11-30) rabbit polyclonal (Santa Cruz); anti-Bax(150-165) rabbit polyclonal (Oncogene Research); and anti-Bax(43-61) rabbit polyclonal (PharMingen) antibodies.

Techniques: Infection, Immunoprecipitation, Marker, Western Blot