Journal: Scientific Reports

Article Title: MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene

doi: 10.1038/s41598-021-93616-8

Figure Lengend Snippet: Validation of candidate gene targets of miR-378a. ( a ) The expression levels of BMP2 and INHBA by qPCR using mRNA from NHEK cells transfected with miR-378a mimic or inhibitor for 24 h and 48 h. ( b ) Comparison of the expression levels of the selected candidate genes from high-throughput RNA-sequencing (light colors) versus real-time qPCR (dark colors) from NHEK cells over-expressing miR-378a mimic (red) or inhibitor (blue) for 24 h and 48 h. Error bars indicate mean ± SEM from three replicates. The experiment was performed three times independently. *Indicates P < 0.05, **indicates P < 0.01, and ***indicates P < 0.001.

Article Snippet: The membranes were cut according to molecular weight of the proteins and initially blocked with blocking buffer (5% non-fat dry milk in PBST) for 1 h. Later, it was immunoblotted with anti-BMP2 antibody (1:1000 dilution; #AHP2442, Bio-Rad Laboratories, Inc.), anti-involucrin antibody (1:1000 dilution; #sc-398952, Santa Cruz Biotechnology), and anti-GAPDH (1:5000 dilution; Santa Cruz Biotechnology, Dallas, TX, USA; cat#sc-25778) for 1 h at room temperature.

Techniques: Biomarker Discovery, Expressing, Transfection, Comparison, High Throughput Screening Assay, RNA Sequencing

Journal: Scientific Reports

Article Title: MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene

doi: 10.1038/s41598-021-93616-8

Figure Lengend Snippet: BMP2 as a novel target gene of miR-378a in keratinocytes. ( a ) Hypothetical binding structure of miR- 378a at 3′UTR of BMP2 (left) and INHBA (right) with predictive binding energy and probability value for actual binding. ( b ) Luciferase activity of wild-type and mutant 3′UTR of BMP2 genes in HEK293T cells co-transfected with miR-378a mimic or inhibitor for 48 h. ( c ) Western blot images (top) and quantitative measurement (bottom) of BMP2 protein in NHEK cells with miR-378a overexpression or suppression for 72 h. A representative image of BMP2 protein from four independent experiments was shown (also see Supplementary Fig. ). ( d ) The expression level of BMP2 in psoriatic lesions (n = 4) and healthy individuals (n = 4). *Indicates P < 0.05, **indicates P < 0.01, and ***indicates P < 0.001.

Article Snippet: The membranes were cut according to molecular weight of the proteins and initially blocked with blocking buffer (5% non-fat dry milk in PBST) for 1 h. Later, it was immunoblotted with anti-BMP2 antibody (1:1000 dilution; #AHP2442, Bio-Rad Laboratories, Inc.), anti-involucrin antibody (1:1000 dilution; #sc-398952, Santa Cruz Biotechnology), and anti-GAPDH (1:5000 dilution; Santa Cruz Biotechnology, Dallas, TX, USA; cat#sc-25778) for 1 h at room temperature.

Techniques: Binding Assay, Luciferase, Activity Assay, Mutagenesis, Transfection, Western Blot, Over Expression, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients

doi: 10.1186/1756-9966-29-85

Figure Lengend Snippet: Correlation of the expression of BMP-2 and its receptors with survival rate and survival time of ovarian cancer patients

Article Snippet: BMP-2 rabbit anti-human polyclonal antibody, SABC immunohistochemical kit, DAB color-developing reagent (Wuhan Boster, China); β-actin rat anti-human polyclonal antibody, BMPRIA rabbit anti-human polyclonal antibody, BMPRIB rabbit anti-human polyclonal antibody, BMPRII goat anti-human polyclonal antibody (Santa Cruz, USA); BMP-2, BMPRIA, BMPRIB, BMPRII and internal reference β-actin primers (Shanghai Sangon, China); Trizol total RNA extraction reagent (Invitrogen, USA); RT kit and PCR amplification reagents (TaKaRa-Dalian, China); alkaline phosphatase-labeled goat anti-rabbit IgG, rabbit anti-goat IgG (Sigma, USA); Bradford protein kit (Nanjing Keygen, China); nitrocellulose filter (Shanghai Gene, China), β-naphthyl acid phosphate, O-dianisidine (tetrazotized; Sigma, USA).

Techniques: Expressing