Journal: PLoS ONE

Article Title: Endoplasmic Reticulum Stress-Sensing Mechanism Is Activated in Entamoeba histolytica upon Treatment with Nitric Oxide

doi: 10.1371/journal.pone.0031777

Figure Lengend Snippet: Genes upregulated encoding metabolism enzymes.

Article Snippet: The primary antibody used were a rabbit anti-PFOR polyclonal antibody (1∶400 dilution, a kind gift from Dr Ester Orozco, CINVESTAV, Mexico), a mouse anti-actin monoclonal antibody (1∶20,000 dilution; #69100, MP Biomedicals).

Techniques:

Journal: PLoS ONE

Article Title: Endoplasmic Reticulum Stress-Sensing Mechanism Is Activated in Entamoeba histolytica upon Treatment with Nitric Oxide

doi: 10.1371/journal.pone.0031777

Figure Lengend Snippet: PFOR of 120 kDa was revealed upon SDS-PAGE and immunobloting of crude extracts from SNP-treated and control trophozoites (4, 2 and 1×10 4 cells). The loaded amount of proteins for each condition is evidenced by actin (43 kDa) immunodetection on the same western blot. Quantification of signal emission did not reveal differences between the tested conditions in 3 independent experiments.

Article Snippet: The primary antibody used were a rabbit anti-PFOR polyclonal antibody (1∶400 dilution, a kind gift from Dr Ester Orozco, CINVESTAV, Mexico), a mouse anti-actin monoclonal antibody (1∶20,000 dilution; #69100, MP Biomedicals).

Techniques: SDS Page, Western Blot, Control, Immunodetection

Journal: PLoS ONE

Article Title: Endoplasmic Reticulum Stress-Sensing Mechanism Is Activated in Entamoeba histolytica upon Treatment with Nitric Oxide

doi: 10.1371/journal.pone.0031777

Figure Lengend Snippet: PFOR activities upon no treatment.

Article Snippet: The primary antibody used were a rabbit anti-PFOR polyclonal antibody (1∶400 dilution, a kind gift from Dr Ester Orozco, CINVESTAV, Mexico), a mouse anti-actin monoclonal antibody (1∶20,000 dilution; #69100, MP Biomedicals).

Techniques: Activity Assay

Journal: Clinical Science

Article Title: Antiepileptic effects of long-term intracerebroventricular infusion of angiotensin-(1-7) in an animal model of temporal lobe epilepsy

doi: 10.1042/cs20200514

Figure Lengend Snippet: Figure 7. Effects of TLE and Ang-(1-7) treatment on hippocampal protein levels Representative Western blot bands (A) and protein levels of ACE2 (B), NEP (C), AT2R (D), AT1R (*P=0.0406 CT vs EP) (E), Mas [*P=0.0406 CT vs EP + Ang-(1-7)] (F), IL-6 (G), SOD (H), CAT (*P=0.0389 CT vs EP) (I), Bcl-2 (*P=0.0468 CT vs EP) (J), and

Article Snippet: Published by Portland Press Limited on behalf of the Biochemical Society 2265 D ow nloaded from http://portlandpress.com /clinsci/article-pdf/134/17/2263/892258/cs-2020-0514.pdf by U niversidade Federal de Sao Paulo (U N IFESP) user on 14 M ay 2024 Table 1 Host, specificity, dilution, catalog number and source of the antibodies used in the present study Antibody Host Specificity Dilution Catalog number Source ACE2 Rabbit Monoclonal 1:1000 GTX01160 GeneTex, CA, U.S.A. NEP Mouse Monoclonal 1:1000 sc-46656 Santa Cruz Biotechnology, CA, U.S.A. AT1 Mouse Monoclonal 1:500 sc-515884 Santa Cruz Biotechnology, CA, U.S.A. AT2 Rabbit Monoclonal 1:1000 M00432 Boster Biological Technology, CA, U.S.A. Mas Rabbit Polyclonal 1:500 ab66030 Abcam, MA, U.S.A. IL-6 Mouse Monoclonal 1:500 IM-0407 Imuny Biotechnology, SP, Brazil SOD Mouse Monoclonal 1:1000 sc-17767 Santa Cruz Biotechnology, CA, U.S.A. CAT Mouse Monoclonal 1:1000 LS-B2554 Lifespan Biosciences, WA, U.S.A. Bcl-2 Mouse Monoclonal 1:1000 sc-7382 Santa Cruz Biotechnology, CA, U.S.A. mTOR Mouse Monoclonal 1:1000 #4517 Cell Signaling Technology, MA, U.S.A. Phospho-mTOR Rabbit Polyclonal 1:1000 #2971 Cell Signaling Technology, MA, U.S.A. GAPDH Mouse Monoclonal 1:2000 sc-365062 Santa Cruz Biotechnology, CA, U.S.A. 2266 © 2020 The Author(s).

Techniques: Western Blot

Journal: International Journal of Molecular Medicine

Article Title: High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic β-cells

doi: 10.3892/ijmm.2013.1469

Figure Lengend Snippet: Schematic illustration of RAS consisting of the classical Ang II/ACE/AT1R axis and AT2R, as well as the two alternative axes, ACE2/Ang-( 1 - 7 )/Mas and APN/Ang IV/IRAP. RAS, renin angiotensin system; AGT, angiotensinogen; Ang, angiotensin; AT1bR, angiotensin II type 1b receptor; AT2R, angiotensin II type 2 receptor; ACE, angiotensin-converting enzyme; NEP, neutral endopeptidase 24.11; PEP, prolyl endopeptidase; Mas, Mas receptor; APA/APN, aminopeptidases A and N; IRAP, insulin-regulated aminopeptidase.

Article Snippet: The antibodies used, antibody dilutions and dilution buffers for western blotting were as follows: rabbit anti ACE2 (LS-B439; Biozol, Eching, Germany) 1:10,000, mouse anti-NEP (CD10; ab951) 1:1,000, goat anti-APA (BP1; ab36122) 1:2,000, rabbit anti-APN (CD13; ab108310; all from Abcam Cambridge, UK) 1:2,000, rabbit anti-IRAP (#6918; NEB; Cell Signaling Technology, Frankfurt/Main, Germany) 1:2,000, all in TBST/5% BSA/0.03% NaN 3 ; rabbit anti-Mas (LS-B2564; Biozol) 1:2,000 in PBS/5% skimmed milk/0.03% NaN 3 ; goat anti -actin (I-19, SC-1616; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) 1:500, rabbit anti-RPLP0 (Biozol) 1:2,000, both in TBST/5% BSA/0.03% NaN 3 .

Techniques:

Journal: International Journal of Molecular Medicine

Article Title: High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic β-cells

doi: 10.3892/ijmm.2013.1469

Figure Lengend Snippet: Effects of glucose on the mRNA expression of renin-angiotensin system (RAS) components. Elevated concentrations of glucose did not alter the mRNA levels of (A) angiotensin-converting enzyme (ACE), (B) angiotensin II type 1b receptor (AT1bR), or (C) angiotensin II type 2 receptor (AT2R) mRNA levels in BRIN-BD11 cells after 24 h, whereas the levels of (D) ACE2, (E) neutral endopeptidase 24.11 (NEP), (F) Mas, (G) aminopeptidase A (APA), (H) aminopeptidase N (APN) and (I) insulin-regulated aminopeptidase (IRAP) were dose-dependently increased. mRNA levels were determined by quantitative PCR. Data were evaluated and normalized to Rpl13a mRNA expression using the ΔΔ Cq method. Data are presented as box plots with medians, quartiles and an interquartile range (IQR) ± 1.5 × IQR of 5 independent experiments [Mann-Whitney * P<0.05, ** P<0.01, vs. control (Co.)].

Article Snippet: The antibodies used, antibody dilutions and dilution buffers for western blotting were as follows: rabbit anti ACE2 (LS-B439; Biozol, Eching, Germany) 1:10,000, mouse anti-NEP (CD10; ab951) 1:1,000, goat anti-APA (BP1; ab36122) 1:2,000, rabbit anti-APN (CD13; ab108310; all from Abcam Cambridge, UK) 1:2,000, rabbit anti-IRAP (#6918; NEB; Cell Signaling Technology, Frankfurt/Main, Germany) 1:2,000, all in TBST/5% BSA/0.03% NaN 3 ; rabbit anti-Mas (LS-B2564; Biozol) 1:2,000 in PBS/5% skimmed milk/0.03% NaN 3 ; goat anti -actin (I-19, SC-1616; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) 1:500, rabbit anti-RPLP0 (Biozol) 1:2,000, both in TBST/5% BSA/0.03% NaN 3 .

Techniques: Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY, Control

Journal: International Journal of Molecular Medicine

Article Title: High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic β-cells

doi: 10.3892/ijmm.2013.1469

Figure Lengend Snippet: Effects of glucose on enzymatic activities of renin-angiotensin system (RAS) proteases. Exposure of BRIN-BD11 cells to elevated concentrations of glucose led to increased enzymatic activities of (A) angiotensin-converting enzyme (ACE)2, (B) aminopeptidase A (APA), (C) aminopeptidase N (APN) and (D) insulin-regulated aminopeptidase (IRAP) after 24 h. Protease activities were determined by the incubation of viable cells with chromogenic or fluorogenic (ACE2) substrates. The activities were calculated per one million cells. Illustrated are boxplots with medians, quartiles and an interquartile range (IQR) ± 1.5 × IQR of 4 independent experiments [Mann-Whitney ** P<0.01, * P<0.05, vs. control (Co.)].

Article Snippet: The antibodies used, antibody dilutions and dilution buffers for western blotting were as follows: rabbit anti ACE2 (LS-B439; Biozol, Eching, Germany) 1:10,000, mouse anti-NEP (CD10; ab951) 1:1,000, goat anti-APA (BP1; ab36122) 1:2,000, rabbit anti-APN (CD13; ab108310; all from Abcam Cambridge, UK) 1:2,000, rabbit anti-IRAP (#6918; NEB; Cell Signaling Technology, Frankfurt/Main, Germany) 1:2,000, all in TBST/5% BSA/0.03% NaN 3 ; rabbit anti-Mas (LS-B2564; Biozol) 1:2,000 in PBS/5% skimmed milk/0.03% NaN 3 ; goat anti -actin (I-19, SC-1616; Santa Cruz Biotechnology, Inc., Heidelberg, Germany) 1:500, rabbit anti-RPLP0 (Biozol) 1:2,000, both in TBST/5% BSA/0.03% NaN 3 .

Techniques: Incubation, MANN-WHITNEY, Control