Journal: International Journal of Molecular Sciences

Article Title: Neuroprotective Properties of Picroside II in a Rat Model of Focal Cerebral Ischemia

doi: 10.3390/ijms11114580

Figure Lengend Snippet: The number of PARP positive cells ( x̄ ± s).

Article Snippet: Rabbit anti-rat Caspase-3 and PARP moloclonal antibody, SABC immunohistochemistry kit, DAB dye were purchased from Boster Biological Company, Wuhan, China.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Neuroprotective Properties of Picroside II in a Rat Model of Focal Cerebral Ischemia

doi: 10.3390/ijms11114580

Figure Lengend Snippet: PARP positive cells in cortex shown by immunohistochemical assay × 400. The expression of PARP was very weak in the sham-operation group ( A ), increased in control group ( B ) and decreased in treatment group ( C ) significantly. Bar 25 μm.

Article Snippet: Rabbit anti-rat Caspase-3 and PARP moloclonal antibody, SABC immunohistochemistry kit, DAB dye were purchased from Boster Biological Company, Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Neuroprotective Properties of Picroside II in a Rat Model of Focal Cerebral Ischemia

doi: 10.3390/ijms11114580

Figure Lengend Snippet: The concentration in brain tissue of caspase-3 and PARP ( x̄ ± s).

Article Snippet: Rabbit anti-rat Caspase-3 and PARP moloclonal antibody, SABC immunohistochemistry kit, DAB dye were purchased from Boster Biological Company, Wuhan, China.

Techniques: Concentration Assay, Control

Journal: Journal of Translational Medicine

Article Title: C/D box small nucleolar RNA SNORD104 promotes endometrial cancer by regulating the 2ʹ-O-methylation of PARP1

doi: 10.1186/s12967-022-03802-z

Figure Lengend Snippet: SNORD104 upregulated PARP1 expression in vitro and in vivo. A Immunoprecipitation of SNORD104 using anti-FBL antibodies. B Immunoblot showing FBL protein levels in SNORD104-overexpressing HEC1B cells. C The 2ʹ-O-methylation level up-regulated RNA in the Nm-seq data set was intersected with the RNA data set of SNORD104-Blast, and the KEGG pathway enrichment analysis showed that the apoptotic pathway ranked first. D Anti-FBL antibody was used to perform RIP assay in HEC1B cells, and the mRNA enrichment of PARP1, ITPR3, TUBA1C, PARP4, LMNB1 was detected by qRT-PCR. E Evaluation of PARP1, ITPR3, TUBA1C, PARP4, LMNB1 protein levels after overexpression of SNORD104, and only PARP1 RNA and protein level were changed. F PARP1 mRNA and protein levels in SNORD104-knockdown Ishikawa cells. G SNORD104 and PARP1 mRNA expression in EC tissues (n = 71) and the correlation between the expression levels (R = 0.3222, P < 0.0001). H PARP1 protein levels in the nuclear fraction of SNORD104-overexpressing cells. I , J PARP1 protein levels in the SNORD104-overexpressing subcutaneous xenografts in nude mice. *P < 0.05, ns means P > 0.05

Article Snippet: Following overnight incubation with rabbit anti-FBL (1:2000 Magna, Proteintech, Rosemont, IL, USA), rabbit anti-PARP1 (1:2000 Magneto, Proteintech), rabbit anti-PARP4 (1:2000, Bioss, Woburn, MA, USA), anti-ITPR3 (1:2000, LSBio), anti-LMNB1 (1:10,000, Proteintech), anti-Vinculin (1:10,000, Proteintech), anti-GAPDH (1:5000, Proteintech) and anti-α-tubulin (TUBA1B/1C) (1:2000, Proteintech) antibodies at 4 ℃, the membranes were washed thrice with 1% Tris-buffered saline-Tween20 (TBST) buffer for 10 min each time.

Techniques: Expressing, In Vitro, In Vivo, Immunoprecipitation, Western Blot, Methylation, Quantitative RT-PCR, Over Expression

Journal: Journal of Biological Chemistry

Article Title: Development of a Novel Azaspirane That Targets the Janus Kinase-Signal Transducer and Activator of Transcription (STAT) Pathway in Hepatocellular Carcinoma in Vitro and in Vivo

doi: 10.1074/jbc.m114.601104

Figure Lengend Snippet: FIGURE 4. A, CIMO activates caspase-3 and induces apoptosis. HepG2 cells (5 105/ml) were treated with 10 mol/liter CIMO for the indicated times, and whole-cell extracts were prepared, separated on SDS-PAGE, and sub- jected to Western blotting against caspase-3 and PARP antibody. The same blot was stripped and reprobed with -actin antibody to show equal protein loading. B, pervanadate reversed the inhibitory effect of CIMO on phospho- STAT3. HepG2 cells (5 105/ml) were treated with the indicated concentra- tions of pervanadate and 10 mol/liter CIMO for 4 h, after which whole-cell extracts were prepared, resolved on an SDS-polyacrylamide gel, electrotrans- ferred onto nitrocellulose membrane, and probed for phospho-STAT3 and STAT3. C, inhibitory activity of CIMO on phospho-STAT3 is mediated by pro- tein-tyrosine phosphatase. HepG2 cells (5 105/ml) were treated with 10 mol/liter CIMO for the indicated times; whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to Western blotting against PTP1B, SHPTP1, and SHPTP2 antibody; and the same blot was stripped and reprobed with -actin antibody to show equal protein loading.

Article Snippet: Rabbit polyclonal antibodies to STAT3 and mouse monoclonal antibodies against phospho-STAT3 (Tyr-705) and Bcl-2, Bcl-xL, cyclin D1, survivin, Bak, Bid, PTP1B, SHPTP1, SHPTP2, procaspase-3, and PARP were obtained from Santa Cruz Biotechnology, Inc. Antibodies to phospho-specific Src (Tyr-416), Src, phospho-specific JAK1 (Tyr-1022/1023), JAK1, phosphospecific JAK2 (Tyr-1007/1008), and JAK2 were purchased from Cell Signaling Technology (Beverly, MA).

Techniques: SDS Page, Western Blot, Membrane, Activity Assay

Journal: Apoptosis

Article Title: Study on the activation of cell death mechanisms: in search of new therapeutic targets in glioblastoma multiforme

doi: 10.1007/s10495-023-01857-x

Figure Lengend Snippet: Primary antibodies used for fluorescence immunocytochemistry reactions (ICC) and western blot reactions (WB)

Article Snippet: PARP1 , Rabbit monoclonal anti-PARP1 (Cell Signaling Technology, Danvers, USA) , ICC 1:200.

Techniques: Fluorescence, Immunocytochemistry, Western Blot