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Image Search Results
Journal: Developmental cell
Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
doi: 10.1016/j.devcel.2018.05.029
Figure Lengend Snippet: Data and Software Availability
Article Snippet: Method Details DNA Constructs The following constructs were obtained from
Techniques: Software, Recombinant, Sequencing, RNA Sequencing Assay
Journal: Developmental cell
Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode
doi: 10.1016/j.devcel.2018.05.029
Figure Lengend Snippet: Data and Software Availability
Article Snippet: DNA Constructs The following constructs were obtained from
Techniques: Software, Recombinant, Sequencing, RNA Sequencing Assay
Journal: Frontiers in Immunology
Article Title: Chlamydia trachomatis Infection Impairs MHC-I Intracellular Trafficking and Antigen Cross-Presentation by Dendritic Cells
doi: 10.3389/fimmu.2021.662096
Figure Lengend Snippet: C. trachomatis recruits Rab14, Rab4, Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.
Article Snippet: The following primaries antibodies were used: rabbit anti-CT529 (gently provided by Agathe Subtil, Pasteur Institute, Paris, France), rabbit polyclonal anti-Rab14,
Techniques: Infection, Confocal Microscopy, Immunofluorescence, Marker, Staining, Membrane, Comparison